FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C14I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C14I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length251
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATGATGGATAAATCCAATCGCAACACATTCCCTCAGATCTTCATCGG11.4084507042253522No Hit
GACCTGGACCGCGATTTTCACGCCGACCGCGAATGTCACCGACCCGAGCA11.4084507042253522No Hit
CATCCTGGATGCAAAGCCACAGAACGTGGTGATCACCGCCGGGTCGCAGT11.4084507042253522No Hit
TTTGAGCTGAGATCATTAATCCTAATGGAAAAATCATCTCATAGAGCATA11.4084507042253522No Hit
GGCTTATGAAGATGCGCCTGTTTACCATGATCCTCCGTCCAATAACCTGT11.4084507042253522No Hit
GTTCTATGTGAGGGCTGCCGGCATCTGGTTTGACCGCATATGCAAGTTTG11.4084507042253522No Hit
GCTGCGCCCGCGCCGTGACACCAGAATGTGCTGGCCTGCAGCAAAGCGTT11.4084507042253522No Hit
GTTTCGGGGCCCGCAAGCTGGAGCATCTGACCGAGCTGAGCCTGTCTCCC11.4084507042253522No Hit
TAGTTGCTCAGGCCCATGTGCAGCAGGCGCTGTTCGTCGGCGCGGTGCTG11.4084507042253522No Hit
CCTATATAGAGGCGCAAAAAAACAACTATGGAGATCCCGGCGAAGTTGCA11.4084507042253522No Hit
CGCAAGGCGCGAATGTGCGCCTGCTCTCCGGCCGGATCCAGATAATGGCA11.4084507042253522No Hit
CTTTCAAATTAGCCCGCATGATCTTTTTAACACCATGCGAATACATACTT11.4084507042253522No Hit
GTTGGAATAATCACGGGTGAGTTCATCCCATAAATAGGCCGTGCCTTCCG11.4084507042253522No Hit
ACTTCCATATGCTTGTAGAATGGAACGAGAAGATGAATTTAACGTCCATT11.4084507042253522No Hit
CTTCACTGCCACGCCCGAATTAGACGGCCCCTTCACTCAGTGATGCGCCG11.4084507042253522No Hit
GACCAACCTGCTGGTACTGAACGCCGCGATCGAAGCGGCCCGTGCCGGTG11.4084507042253522No Hit
AACAGGGGCTCGGCCGAGACCGAGCCGCGTACTCCGGCTTCCTCAAACGC11.4084507042253522No Hit
TTTCAACTCTTTTCCAATCGTTTGACTTACTGACGCCATCGCTGGCAAGC11.4084507042253522No Hit
CCTCTTTATCATATTAATTTACTATACTTTATTTAAATATGACTTTCAAT11.4084507042253522No Hit
CCCTCTGAACATTTAAATTAACGAACGTCTCATAAACACGTGCACGCCTT11.4084507042253522No Hit
GACCTGTACGCGCCGGTCGAGGTGGCGTTGGCGTTTACCGGGATGAGCAA11.4084507042253522No Hit
GATCAGTGCGCAGGCGAAGGCGCCGGCGATGGCCACCAGGGAAAATGCGA11.4084507042253522No Hit
ATCATAATTTGTTGTGATTATATGTACTGGATTCATTGCTAATATCTTGT11.4084507042253522No Hit
TCGGTGCGTTGCTCAAACAATGGCTGCCGCGCTGGCCGACGCTGTTGATG11.4084507042253522No Hit
ATTATTCACTGCATCTCCAACAGCCTTTGCATTTTCACGCTTGAGAACTT11.4084507042253522No Hit
CTATCAACTGGATATGCCAGGCCTGATCGTGCATGCCGAAGACGACACGT11.4084507042253522No Hit
GACCAGGTTGAACAGCCATTCGGTGACAGCTGATGTCTTGGAACCGAGGT11.4084507042253522No Hit
ATTGTAGAGGCAGGGATTTCATTTGGTGCCACAAGCGTTCTGCCGAGCGT11.4084507042253522No Hit
GTGCTGAACATGGTGCGCAACGCGATGCAGGCCATCAGCAGCCAGAACGA11.4084507042253522No Hit
CTCCAAGGGGGGCGGATGTGCTTTTTGAACATTGCTCAGGTGTTGTTTGG11.4084507042253522No Hit
TACATTAAGAGATTATCAAATGCTTGGCATCATTCATTTATTAGCCATTT11.4084507042253522No Hit
GAAAAATTGAATGCGCCTGCGACCCATCGCCTCCCAAGGTTCTGGATTGC11.4084507042253522No Hit
GTTACGTGGTTTATCCACATCACACCCGCGGTGAAGGGCTGCGTAGTAAG11.4084507042253522No Hit
GCGGCGTCGGCTGTCTGTTACATATTTTTGTTGTTTTGTGTGCTGTTCTT11.4084507042253522No Hit
GTAGGATAAGACTACCTTTCTTTTTGTAGCTCACTGCTAGTGCACGTACT11.4084507042253522No Hit
GCAGAAACCCAACTGCCCCAAAGGCCAGGTGTTCGACACCAAGACGCAGA11.4084507042253522No Hit
CCTGATGGCGAGCCTGTTGATGTCGGCGCGGCACAACCACTCGGGCGCGC11.4084507042253522No Hit
GGTAGATCGCACCGTTGATTTTCAGGGCACTGAACAGTTTCTCGGACGCC11.4084507042253522No Hit
GCTTGAGCTTGCGCCGCAGTTTCCATTTCAGGTGCGGGTCGATCACCGTC11.4084507042253522No Hit
ATACAAGAAAGCATCAGCCATATCCGTACGGAAACTACAGCATTTTTGTA11.4084507042253522No Hit
GAACGCCGTGTCTGGGAATCAAAGTTACCGGCACGAAGTCCCGGAATCAA11.4084507042253522No Hit
GATTAGCGCCATCTGGGTGATCGCCGGGGTGAACTGGCTCATGTCCCGCC11.4084507042253522No Hit
GGCGAACGATGGCAGCTTCGCCGAGTACTGCCTGCTCGACGCCGCCACCG11.4084507042253522No Hit
TCTATGGGGTACGTGGCACCAGGAAGCTGCTCTCATTGGTTGGCCTGCCT11.4084507042253522No Hit
TTGCCGAGCTGACCGATAGTGAGGGCCGCAGCAGCTATGAGGCCTTTGTT11.4084507042253522No Hit
CATACTTGGTGTATCAGGATAAGTGAGATCCGTCTGCAGATCTCCTGCTG11.4084507042253522No Hit
GTACCGGCCTTGGGCTTTATCTGTCCCGTGAGCTGTGCGAAAGCAATCAA11.4084507042253522No Hit
TGAAGAACGAGTTTCAAACCTAGTCGGGGCGTAGCGCAGTCCGGTAGCGC11.4084507042253522No Hit
ATGTAAAAAGAGAGTCAACGTGACAGTTTGTTCTATACAAATACTAGGTT11.4084507042253522No Hit
GTATTCATCATCGAATCCACATCACGAAAACTGAGGACAAACATCATCAC11.4084507042253522No Hit
TCAAAAGGATCATAATGATCGGCTGTTTCACTATATTATTTATTCACCTG11.4084507042253522No Hit
CTTAAACAGCCTGTTTATGGCGGCCTACAGAAAGGTTTTAGTCTGTGTAA11.4084507042253522No Hit
CGTTTTACAACAAGCGGATAAACAAGGGACAGATCCTGTCTCTTATACAC11.4084507042253522No Hit
CTTTATTTCTTGTTTGTTTGGGGTACTATGCTACTTCTGCTTACTCATTG11.4084507042253522No Hit
GGTGTTATCGAAGGTAACGTAGCGACTGTAGAAGAAGTGAAGGCTCTTGC11.4084507042253522No Hit
GTTTAAGCGGATGATAGAATACATCCAGTCACGGGAACGACCAAAAGCAA11.4084507042253522No Hit
CCTTCGGCACCGCAGGCGGCAGCGTCGCCGTTCTACTTCGTGGGCAACAG11.4084507042253522No Hit
ACACAGACAGCGGCAGTGCGCACGGCGCGCGCCGACATCAGGTCATTCAA11.4084507042253522No Hit
AGTTGGTGATCTGCTCGCGGCGACCGGTGTCGATGTTCTGCATGAAGATG11.4084507042253522No Hit
TGTTCGGCACGTAAGGGATGTCGTCGCCGAAGAACCACAGCACCGAGTTG11.4084507042253522No Hit
GGTGAGCTGTCTCGTTCGCTCGGAAAATGCTGCACCGCAATGTCAAAAGT11.4084507042253522No Hit
TCCCTTTGCCACTTCATCTTTGAAATGATCCTTATTTTCATGATGAAAAT11.4084507042253522No Hit
CTGATAATGTTTATACACAGAGCGCAGACGTTCGATAAACAATGAGCCAG11.4084507042253522No Hit
GTCCATGTAGTACTTGTTCTCGAGCAGGCGGTAGATCGGGCCGAAGGCAC11.4084507042253522No Hit
CGGCCAGCCTGGAAACCGTGGCCGGGCCGATCGGCCACGCGCTGATTGCA11.4084507042253522No Hit
TGTTAAAGCAATGCCTTTTCCCCTTTATCGCCCGGCAAGGCGGCGGACTG11.4084507042253522No Hit
GGCTTCGGGAGTCTTCTCGCCCAGGAGCGGGCGGAAGCCCCCCTTTTCGG11.4084507042253522No Hit
ACCTGGTACGCATCGGCGTGGTCGACGAGTTCGAACTGGGCGACCCGCCG11.4084507042253522No Hit
AGCGATTCCAGTTCCCAAGGCGCGCGGTCTGCGGCCACCACGACCTGCTT11.4084507042253522No Hit
GCTCTTCATCGCCAACGCCGACGCGCTGGTGAAGGTGCCCTACACCGAAG11.4084507042253522No Hit
AGTTGAAACACAGCTCCAGCGGATCGAGCAGGTCGCGGTTGTTGTGCAGC11.4084507042253522No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)