FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C14I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C14I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGCGGTGCCGCCGGCCATCAGAATCGCCGTGAGCAGCACGCCGAA11.2048192771084338No Hit
CCTTTTAGTGAGATGACACATGGGTTTGCTCCGCGTGCAACGACTTCTTT11.2048192771084338No Hit
GATGATGATGGTGTCGTTCAGCGAGTAACCCAGCATCGCCAGCACGGCCG11.2048192771084338No Hit
GTACAATGAATCCGCCCTCCCCTTGGACTGGCTCCATCACCACACAAGCG11.2048192771084338No Hit
GCGTATCAGGCCGTCGTGATCGCCGACGGGCCGCTCGCGCAGGAACAGCT11.2048192771084338No Hit
GACCGGATGAGAGACGAGTCGCCTGCCACGGCCGCCGCGCCCAGCGCATC11.2048192771084338No Hit
TCCGGTAGATGCGCGCGGGCTGGTACTGCCCGGCGTCGTCGAACGTTCCC11.2048192771084338No Hit
AAACGAAGGAGAGCCAGCCAAAGCGGAAATCACAGAAAACTTTGTGGTCA11.2048192771084338No Hit
TCCATGAACAGCGAGTAACGCGACATGCCGAAGCAGAAGATCCAGAATAC11.2048192771084338No Hit
GGCGACGGCTGCACAGCGCGCAGCGTGCCGTAGTAGCGCTTGTCGGGGTC11.2048192771084338No Hit
CGTTTTACATAGTGCAGGTATCGGCTCCCGGAGTTCGGTGTTTGCGGTTG11.2048192771084338No Hit
GCTGTTGTTGTGGTCGACCTTGATGGTCGGATCGCTGCCGGCAATCAGGT11.2048192771084338No Hit
GCACTGACGCCCAGCCCGCTCGCCACCATCCGCCCGACCGTCGCCAGTTG11.2048192771084338No Hit
GATAGGTAAACGGGGCGTTCGCGCTATACGCAGAATCGCCACATGAAGGC11.2048192771084338No Hit
GTCCCTTTACGGGCGAATCACAAATTGCCATCGCACTGGCGCAGGTGAAC11.2048192771084338No Hit
GGCCAAAGGTGTGCCCGGCGTCAGTTCGGCACTGGCCGAGCGCCTGACCG11.2048192771084338No Hit
GTGCCGTTGCCGTGGTGCACGTCGATGTCGAGGATCGCCACTCGGTCGTG11.2048192771084338No Hit
GTACATCTACGGCAGCGACTCCACGACGTTCATCAAGCTCTCGGTCGAAG11.2048192771084338No Hit
CCCTCTTGATAGTGCCGTCATTACAGTTGGTACGATTACAGGTGGCACTG11.2048192771084338No Hit
CATGTATGCTTTGATTGCTGATGAAAGGCGTTTATCGCTGCCTCTTTTTC11.2048192771084338No Hit
GTTCGATCCGGCGCGCGGTTCGGCCCGGGGCTGGATGTTCAGCGTGACCC11.2048192771084338No Hit
GTACACAATTGTATCTCTACTGTCTATTTTTCACGCAGATAAAAAAAGAC11.2048192771084338No Hit
CTCTGGGGGAAGGCCGGGATGGGGGCAGGACCTCGGATAAGCGCAATGCG11.2048192771084338No Hit
ATCGAACATGTCCACACCGCGGCGCACACCCTCAACCAGATCTTCCGGTT11.2048192771084338No Hit
CAGTCCTACAAGCAGGTCTTTTACCCGCACCAGGTCATTCAGGAGTGCAT11.2048192771084338No Hit
CGTGATTGCAAGCGTCATTCTTGCAGCCATCGCGTTCTTCGGAGCGAGCC11.2048192771084338No Hit
GAAATGACCGCACCGACGCCAAGCGCCCAGAGCGACCAGACGCCCGCGTA11.2048192771084338No Hit
TGCATAAACTATGTGCGGCAGCCAGTTTATGCGCCATCCGTGTGCATCTC11.2048192771084338No Hit
CAACACGCCGCTGGATGATCAGGGTGATGTGCTGTCGCGAATCAATCTCG11.2048192771084338No Hit
TTCCGATACATTCTAGACCTAGTTTGACAGCCTCATCGATATATTCTCTG11.2048192771084338No Hit
GGCCTGGTCCGTACGTGCGGTGCGCTGCAGCAGCTGTGCATACCGCACGC11.2048192771084338No Hit
CAACATCTCCCTGGCACGCAGTCCCTGATAACTCTTGAGCGAACCTCCTA11.2048192771084338No Hit
AGACTGCTCTGTGCCTGAGAACATAGGGACTTCACTTGTCTGGCCGTTTT11.2048192771084338No Hit
ACTTCGGCTTGTACAGGGACCGCGTCGGCGCGTTGATCGTGTGTGCGGCG11.2048192771084338No Hit
TGGTTGAATCGTATGCAGTGAAAGCAGGATTAATGGAAGACATTGTGTCT11.2048192771084338No Hit
ATACATCATAGATGAATGATCCTGACCAAGAATGTCAGGCAGCAGAACTT11.2048192771084338No Hit
GGGGTTCTGCCATGGGGTTCCTCTTTGTATGTAGGGCGCTGGAAAAAGTG11.2048192771084338No Hit
GGTCCGGACGATTCAGATAGCCACGGGCCAGGCAGGCGCCGGCGATGTAC11.2048192771084338No Hit
CTGGTGCACGAGGCCACCTTCACGCAGGAGGCACTCGACAAGGTCGGCCC11.2048192771084338No Hit
GAAGTATTGTCGTTTCGTTTTCTCTCAATTATCAGTATTAGTGTGTATGT11.2048192771084338No Hit
GAAGATTCTCGAACCCTTGCGCGGCCAGATTCCCGACGAGGTGTTTACCC11.2048192771084338No Hit
ATTTACATCAGGGTTTTACGAGGGGCGGGATCGAACCCGGAGGGTGGGTC11.2048192771084338No Hit
TCCATGTCTTTCTCGAACCAGTCGCCCAGCCATTGGCTGTATTGCCTCTG11.2048192771084338No Hit
GATACATATTGAAGCATTAGATGTGACAGATACACAGTCCATTCAAGCAT11.2048192771084338No Hit
CTTCCTGAGACCGTCGAGTATCCCGATCACCCATGGTTCTGTCTCTTATA11.2048192771084338No Hit
CGGTCTCAACGCCCGCGAACCTTTCGAGGTGGCAGACGCGCGCCCGGCCC11.2048192771084338No Hit
ACCGTGACTGCCCCTTCCCGTCGAGAATGGCAGTGAATGGCTGGAAGAAA11.2048192771084338No Hit
AGAACAGACCGTTTCACAAAACCGTCCCATTGAAGAAGATTTATTAAGTC11.2048192771084338No Hit
GTGCGTGGCGTTCACGCACAGCATCGAACGTTGTCACATCGGTGTCATTC11.2048192771084338No Hit
CTTTTCCTGCATGGCCGTGAGAAAACTGTCGTGAGCCTCGTTCCATTGTT11.2048192771084338No Hit
ATTTATAACGAAGCTCATGTCCGGCTCGCGATACTTATTTAGCGGTGTCG11.2048192771084338No Hit
ATATTTGCTGGCGTCTCTTCTTTACATTGCCGCATGAAGGTACTTTTAAT11.2048192771084338No Hit
GGTGATGAGACATGCACGTTTTTCTTAAAGAATCTTTTGATACATGCGTG11.2048192771084338No Hit
CTGCAGCGCGCTTGCCATGTCGGGGTCGTCGGTCAATGCGCGCGTCATCG11.2048192771084338No Hit
CGCTGAACTGCGCGCATTCGATCAACAGATGCTTGCCCCAGCTGCGCAGC11.2048192771084338No Hit
TTCTTTTCTCCTGCCAAATATCCAGCAGTACATCCTGTAAGTGTTTCCGT11.2048192771084338No Hit
GGAGAACTCCCCCACCCGCTCGAAGATGGCGCCGCGCAGGTCCCGACCAA11.2048192771084338No Hit
GCCTACATCGGCGGCGACGTCACGGTGATCGGGCCGAAAGTCGCCGGTTA11.2048192771084338No Hit
TTCTGCTGTTCGGTGATCGCCTGATCGACGAGCCGCGCCTCAAAGTCCCG11.2048192771084338No Hit
ATCGCGTTGGGCACATAGCCCAGTTCGCGGATGGCGAGCTCCACGCGCTC11.2048192771084338No Hit
CTCCTGGTGTGCACGGCAGTCTCATCGGAAGACGACCAGCCATCATCCTT11.2048192771084338No Hit
GCTTCCAGCGGATAAGCGCCGGCAGCGGATTCGGCAGACAGCATCACGGC11.2048192771084338No Hit
TATATCTGCACTTCTCAATGCTTTCTTCATGGCTAAGTAGGCACCCCTAC11.2048192771084338No Hit
CACGAAGCCATGCGCAATGCGCTGGCCGATTTCGCTTTGCGCCAGCGTCG11.2048192771084338No Hit
GTTGCTGGGCGAGGCACAAGCGATGGGCAGTTGCGTGGCGGGAACCAACT11.2048192771084338No Hit
CCACTGCACAGGGCGCCGGGTGCTCCCCGCAGCGAAATAAAGGAGGAGGC11.2048192771084338No Hit
TATCGCAACTGCCAACAATTTAGCAACCATTCCAGGTCCACTTCGTGATA11.2048192771084338No Hit
CTCCCTGCATAGTCTGATACTTGCATCGATTAATATTTGGATTTTATTAA11.2048192771084338No Hit
CATGGTGCTAGAAGCGCACGCCGTTCTTCTGATTCGCTCTCTTGTAGTTG11.2048192771084338No Hit
ATCCGTATTTCCGCAGCACCATCGGACAAGTTCACGATTTGGCGTAAAAT11.2048192771084338No Hit
GATAAACACAGCCGCTATCTCGAGGCGGCAGTGCACGGGGTGTTGGTGGG11.2048192771084338No Hit
TAGTAGTCATGTCCAAGCTCGTCCATTGCATCTTTCAGCATAGACTGTGC11.2048192771084338No Hit
CTCTCATATCGATTGACGGCCTCGATGCCGAATTGCAGGCCGAGCGTCTT11.2048192771084338No Hit
GAGCCAATCACCGTGTACTCCGCGCGCTGGATCGAATGCAACGACAAGAA11.2048192771084338No Hit
GGTCTGGTGCGTTATCGCGCGGCGCTGCTGATCGCGGCGCTCGGCGCGGT11.2048192771084338No Hit
ATCTGGTTTATCTCGCGATCAAGCAATGGCGCGCACAGCCGATGGACATG11.2048192771084338No Hit
GAGGACCGCACCGGCGACGACTTCGACGAGAGGATCGCGGCCGCGATGCG11.2048192771084338No Hit
CGCGTGATTCGTCCGGCCGGGAGGGGTCGGCGCTGACGCGGCGCACCGCG11.2048192771084338No Hit
GTGCTTGCCTATTCTCTTTGTGCTGCTGGTGGCGCTGCTGCTTGTGCATC11.2048192771084338No Hit
GCCTGTAGCACGACGCATTGGCCGTGCCGCAGGTGAACAGCACCTCGAAG11.2048192771084338No Hit
GGCCGTCGGCGGTACCGGCGCACTGAAAATCGGTGCCGACTTCCTCAAGC11.2048192771084338No Hit
GGTTTCCCATGCGTGTGGAGCTAGTACGAATGGCTGAAATTAAGCTAAGC11.2048192771084338No Hit
CGTAGGTGTGGTGGTCGACGATGTTGTGGCCAGGACGGTTGAACTGCGCG11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)