FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C14I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C14I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACCGTCCCTTTGCCCTTCAACCCTTGCCGCGCGTACAACAGAAGCACG12.380952380952381No Hit
GAGGAGATCTCAGCGATGCTCGCTACTGGATCGAAGAAGCGGCCAAGACC12.380952380952381No Hit
GTCCGGTGGACGATCCGTTGATTGCGCAACAGGGCGCGGTGTTGCTGCTG12.380952380952381No Hit
TAAGTGAACGATCATACAAAGCGTTAAATGACCATTTTTCCTTAAATATG12.380952380952381No Hit
ACATATATACGCCCAAAAGGCGCTGTGAGAATCGGTCCATAGAAAGGAAG12.380952380952381No Hit
AGACCCCACTATCCGGAAGAACTTACGCTTGCTAGTGAAAGTAAATAACA12.380952380952381No Hit
GAGGTGTATTGCAGGAAGGCGATGTGGTCTTGCGGTAAGGCCGGTTCGAC12.380952380952381No Hit
CTGTTCGTGGTGTCGCTGGTGGCGGGGCGGCTGGCCGACCGGCTGCCGGC12.380952380952381No Hit
CCCTGGTGGGCGTCAGCAGCACCGAGACCCGGCCGCCGTCGGTATGGCCG12.380952380952381No Hit
GATCCGGCGTGGCACTGAAAACGGGAAGCCTTCGGGTTTCCCGTTTTTTA12.380952380952381No Hit
GCGCGGGCGAGTGCGAAGCTGGTCGCCATGGTGAAGCAGGCGGCGTGCGC12.380952380952381No Hit
CTTCTCGACTGGCCGGTTCGCGCGGCACTGGCGCGGCTTCGGGCGTGGCC12.380952380952381No Hit
ACGCCATTCTGTATGCGGGCATGAAGCCTGTATTCGCGGACGTCGACGCA12.380952380952381No Hit
ATCAGCGAGAGCCATCCTGCAGCGCCAAGCGTCAGGTTATTGGCCACCAT12.380952380952381No Hit
GCAAAGAGCCGCGCATCGTGCCAGTCGGCCAGCATGCGCCCGTTGCTCAG12.380952380952381No Hit
TTTCAATCCTTCTTCAAGCTGACCGATTTTAATCTGGTGGATGCCATCTA12.380952380952381No Hit
TCCTAATATCGGCGTGGCCATTGTCAACTTATTACAAAATACCTCCCTCG12.380952380952381No Hit
ACAATCGTGCGGCTTCACCCTTGGCTTTGCCCAGATTGTTCTGCCAGAGT12.380952380952381No Hit
GTTCAAATCCTCTATCAAAACGACTAGTTTTCGCCGTTTCCTCCATGATC12.380952380952381No Hit
CGCCGGGCGTGGCCATGCCGCAGATCAAGGCCGGCAAGCTCAAGGCCATC12.380952380952381No Hit
CCTCTACATAAGGAGTAATTAATTTCTCAAACTGCTTTTCATCATCCACT12.380952380952381No Hit
CCACCACGGGATTCGCAAAGTTCTGGGGCACGGAAGCCCAAAACGTTCAT12.380952380952381No Hit
ACCGCTGGCCGACAACAGATACGCCAGGGCAAACCCGATGTGCTCCGACA12.380952380952381No Hit
AGCTGAATGCGCGGCTGATGGGCGTGGCCCGCACCGGCAACGGTCGTCGC12.380952380952381No Hit
CTGTATGACCAAGCACACGCACACCCAAACGCACGACCAGGAACCTGTCT12.380952380952381No Hit
GCCCTGGCGCAGGCCCTGGTGGCCGCGCCCGACGTGCTCCTGCTGGACGA12.380952380952381No Hit
GCCTGGCAGCGCCTCAAACTCAACGTACAGGTGGCCACCAAACTCAACCA12.380952380952381No Hit
CAACAATATCGTCGGCCACACGCTGGGCCATTTCGCTGCGTGAGAGTTTG12.380952380952381No Hit
AGACCAGACTGCGCAGCTTGGGAATATCCGCCAGGTAGAACCACAGATAG12.380952380952381No Hit
GTTAAAGCAAAAACAAATGGAATCTATTTGTTTAAAATCTATTATCCTAA12.380952380952381No Hit
GGCCGATACGCTGCTGCTGGCCGGTCACGACTATCACGCGGTGGGTTCGG12.380952380952381No Hit
GGTCAGTACTGCCTGAGCAATGATGATCACCAGCAGGGCGATGATGAACG12.380952380952381No Hit
CATCGAGACCAGCCAGGCCGAAGTGCGCAAGATCACGCTGCACCAGGTGC12.380952380952381No Hit
CATCTTGAGTGCACTTATTTAGACACTCGAAACTTAGTATGCCTGCACAC12.380952380952381No Hit
CAACAAATCGGTGTCGTTGGACTAGCCGTTATGGGGAAAAACCTTGCCCT12.380952380952381No Hit
CCTATTTATGCTAGAGAACTCAACATATTCAGTGATTTCACCTGAAGGTG12.380952380952381No Hit
GGTCTTCATGAGGCTCGCGACGGTGGCGACGGCGATCGTCGCGCCGATGA12.380952380952381No Hit
GGAGCCGTGACGCAGTCGGCGCGGGCGTTCGCCGATGCAGCGGTTCAGCT12.380952380952381No Hit
GGGCTACGGCAAGCTGATGGTCGATGAGAAATTCGAGCCGGTGGGTATGA12.380952380952381No Hit
GAATTAGACAAGATCGACATGATCGTTTGCACGAATCAGAAAATCTGCTG12.380952380952381No Hit
ATCGAATCGTGATCTTTGGAGCAGGTACAGCAGGGATTGGTATAGCCGAG12.380952380952381No Hit
GGCAAAGGTGTCGATCTGCAGCAACGGGGTCACGGCCAGAGGCGCGACTT12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)