FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C13I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C13I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCATGCGAATGACCTGGCGCAACATATTGATCCTCGGACACAACATTG11.5384615384615385No Hit
AATGAAAGCTGGGAAGGCCGCATCGCCTTTGCCGGCAAGGATGCGCCGCT11.5384615384615385No Hit
ACGACCGTGCCGCCACCGGGCATGGCGTCGCGGGCATTGGTCGCCAGATT11.5384615384615385No Hit
CATCTACGCGGTGTTGTTCTTGGTGGTACTGGCGGGGCTGGACGTCAAGC11.5384615384615385No Hit
GCAGCATCATTTCCGTTGCGCTTTTCATAACCGCCTTCTACCACCCGCTT11.5384615384615385No Hit
GCCCAAGCCGGGCCTTGGCAAGGGCCAGCCAAGCCCGATGATTACACTGG11.5384615384615385No Hit
GGAGATCCGTCGGCTGTTGCCTTCGGTGTTCATTGCCGACGGTGGCTGTC11.5384615384615385No Hit
TAATTGCACCGGTGCCGGGTCGCCGGCTTCGATCCAGTAGGCAAGGGTGT11.5384615384615385No Hit
TGTTGAAACGATGGCGCAGCTTGTCGAGATAGAGGTAAACCACCGGGGTG11.5384615384615385No Hit
ATCTTGCCGCCCGCGTCGCGCACCGGGGTGTACTGCGACATGTAGTAGCG11.5384615384615385No Hit
GTTCGTTACCTGGCGGCGCAGATGAATGAACATGGCGAAACCTATATTGC11.5384615384615385No Hit
CATCGGAGTGTTTTTGGTCGTCACGAATGGCAGTCATCAGGGCCAGCATC11.5384615384615385No Hit
GTTCAAGCGCAGGCTGTTAAGGCCCATGCGCGAGCGGTTCTGCTCGAGAA11.5384615384615385No Hit
CAGCAGAACGCCGCAGTTCTCGCCAGCACGACCTTCGTCGAGCAGCTTGC11.5384615384615385No Hit
AACTTGCTGTGGCGGGCGAGAATGGCCAGAAGGTGAACTGGGTGAATGTG11.5384615384615385No Hit
GTTGAGAACTGGCGCGAGCGCCTGGACGGCATTGAGGTCGAGACGGTGAT11.5384615384615385No Hit
TCTTCGCGATGGCCGAATCGGACCTGATTCTGGTGCTGCTGTCGCTGATC11.5384615384615385No Hit
GGTGGATGGGGATGCCGTCAATCGTCCCGACCACTACGAACGGTTCGCCA11.5384615384615385No Hit
GCGCGTGGCAAACCAGCGAAACGCCTGGGCGCAGACCACCGCATCCACCG11.5384615384615385No Hit
CCCCAAGCACACGACGACCCAGGCTGGCCAGCCATATCAGGTCCAGGCTT11.5384615384615385No Hit
TCTGTAAACAGGTATTTGGTGGCTACACAGGGACTTGAACCCCGGACCCC11.5384615384615385No Hit
ATTCTTATCCCTATGTGTACAGGATAGTGGCAATTTGCCGATTTACTAGA11.5384615384615385No Hit
ATCTCGAAGAAATCGAGGAAGAAGGCGAGGAAGAAGATGAAGATGTTGAC11.5384615384615385No Hit
GATGAGATCAATGGTCGCTGGGGAAAGGGAGTGCTGCGTGTAGCCAGCGT11.5384615384615385No Hit
TCCATGTGACGTTCTCCTGAACAGTTGGGCTGTGTCAGCCGATGATGATG11.5384615384615385No Hit
GGCTGCACCCGCAACTTTCGACGACGCCGACACTGGCCCACCCGATCAGC11.5384615384615385No Hit
CGACCGGTTCGATCAGGCTGTGGCGGTTGCACCTTTGCAACTGGGCGGAG11.5384615384615385No Hit
GTTTACACAGCGTGGGAATGAATGACCCGGCAGTCACCCCTGACGAACGG11.5384615384615385No Hit
GAACGGCAGCCTGTAGATCTCGAACAGTTCACCATCCGGTGAAAAGCGGT11.5384615384615385No Hit
ATCGTGGACTGCTAGACACGCTTTCGACCTACGCGCGGGAGGGCAAGTCG11.5384615384615385No Hit
TCGGTAAACCGCGCAATCGGATCGAACGTCAGGCTGTGATGGTTGGCCTC11.5384615384615385No Hit
ACCGCGAACTGGGCGCCGATTTCGAGGCCCCGACGCCGGTGTCCAACCGT11.5384615384615385No Hit
GGCAATATGCCTGTTCGCGCATGACGGTGAACAAGGCTCTCGGCGAACTG11.5384615384615385No Hit
GGTTTTTACGGCGTGGTGGTCGCGGCGTCGCTGATCGCTTCGAAGTTCTA11.5384615384615385No Hit
GGTATAGAGCAACGGATGCGACAGGTTGGCCGGTTTGCCGCCCTTTTCGG11.5384615384615385No Hit
CAAGGAAGGTCTGGATTGCAGCCACGTTGAAGTCGATAAAACCCATTCGA11.5384615384615385No Hit
GTAGCACTGCGTGTCAGCGTTATGGTTTGGCCCAGAACAGGCGCGGGAGC11.5384615384615385No Hit
CTCTTTGTACAAGTCCATCGCCTCGGCGCTGGCGGAAAAGTCGGCGAGTT11.5384615384615385No Hit
CCGTCAAAACGCCAGACCGTTCCGACGCGATCCGTCGGTCCTCGATCCGC11.5384615384615385No Hit
GAAACAACGCAATGTCGTCGAACAACGTCGCCGGATACAACCCTGTCGTC11.5384615384615385No Hit
ATTGTCCTTTACCCCCTTTGAACCTACGATCCGTTTGAGATTTCAAACGC11.5384615384615385No Hit
GGATAAACAGGTGTTATTGGATGTCTGGGAGGTTTCGGCCTTCACCGGTG11.5384615384615385No Hit
CTGGAACGGCACGTCAGGACGGTCCTGGCGACGTGGATACAGAGGCAGAG11.5384615384615385No Hit
ATTTCCATCGGTTCGAATTTCCGCGGCGGCCTGTTTTTCGCCTCGCTGTT11.5384615384615385No Hit
GAACAAGCCGCGGTGGCGCGCGAGGTGGATCGCAATCTGATGAACATCCG11.5384615384615385No Hit
GATATTTCCTCACAGTATCCTCCGTTGAAAATGCAGTGACCCAGCGAAGC11.5384615384615385No Hit
GTAATGGGCTCTGGAAATGCCGGCATTGATTCCTGTCGAGCGTGTGCCGG11.5384615384615385No Hit
CTTTCATGTGAAAGGGGCTGTCATCAATTTATGCGTCAAAGGAAATAAGA11.5384615384615385No Hit
ATCTACAACTTCCCCAGTGGCGGCGGCGCCGGCCAGAGCGTGGCCATCCT11.5384615384615385No Hit
ATCCACAGGTCGCCGGACGCGGTTTGCAGCGCTTGGCCGAGGCCGGTATC11.5384615384615385No Hit
GTTCTGGTGCAGTCAGTTTGATTCTTTGGGGCAGTGAGGACGATATCGCG11.5384615384615385No Hit
TACCAGATCTGGCTGATGTTCGAAGGTCAGCAGCAAGTCGGCCATGGCGT11.5384615384615385No Hit
GCCGTTCAATCACTTCATCGCTCAAGCCCACGGAGTGGCCACCATGTCCG11.5384615384615385No Hit
CACCAGACAGGTGATCAAGCGCGAACTGGCCACTGTCTCTTATACACATC11.5384615384615385No Hit
GTTCAGGATGAGTATTAATCATGCCACCCATCCTTTTTATCCCTTTGTGC11.5384615384615385No Hit
GCTGTATACGCATCCGCAGAAGACCGTGCAGGACAGCTCACCCTACGTGG11.5384615384615385No Hit
GCAACGCAGTAAAAGAGCATGGCTTCCAGGCCCATGTGGGCTCCATGCGT11.5384615384615385No Hit
GGACATGGCGCGCCGCTACCCGGAAGTGCTGCGCTACAAGCAGGAAGTGA11.5384615384615385No Hit
GAGTACGTATTGCCCGAACTCAAGTACGACTACGCTGCCCTTGAGCCGCA11.5384615384615385No Hit
CCCAGGCCCAGCCCGGTGAGAAAACGCAGCACCAGCAACTGATCGACGTT11.5384615384615385No Hit
AGACTAGAATCGCACGGCACGCAACGGTAGCCACTGCTCTCAAGAAACTC11.5384615384615385No Hit
CTTCTATGCTTAGCTCCGTAACGTCCTTGCTTTTGAAAAACCAAGCCAGA11.5384615384615385No Hit
GGGATGGTAGTCCACTCGGGACAGGTGCCCCTTGGCATTGATCAATCGCA11.5384615384615385No Hit
GCACAATTGCGTTGGTGGCTCAGTCAACGACACCACCTTGGATGTAGCAG11.5384615384615385No Hit
ATGAAGATCCGCAGTGCTTCGTCTGGAAACCGGCCGAACGCGTCATCGCC11.5384615384615385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)