FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C13I3a_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C13I3a_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGATGTCACCGCAGCGCCCCGTCGCCGCCGTGACGGCGCACGACACCC14.545454545454546No Hit
ATCATATCATTGGCCGGGTGGTGTGTGCGCATTTAGATGAATCCGTCTAT14.545454545454546No Hit
ACCGTAGCCAGAAAGAGAATGGCTCCAAGCGCTTCGCGCTGCTCCTAACG14.545454545454546No Hit
AGCGTTTCTGTTCGTGATGTTCCTGATCGTGGTATTTGCCGGCGGCACTT14.545454545454546No Hit
CTGCTCGACGAACCGCTTTCGGCGCTCGACGCCAAGATCCGCGTCTCGCT14.545454545454546No Hit
ATACACATGGCACTGAGAGGCCGCTATCGCGAGCAGGCTCGCTCCCACAG14.545454545454546No Hit
ATTTAAAGCCGGCGGACGCAACGGTGTGAACTATTATACAAATATCGCAA14.545454545454546No Hit
GTACTACACCGACGCGCTGATGAACACCGGCGAAGGCCTGGACTGGAGCA14.545454545454546No Hit
GGGGTGATGACCGCCATTGCCTGCGGCTGGCTCGATGACGGGACGTTCAC14.545454545454546No Hit
GGGTATTTGTCTCGTATCAAGATGTTCGGCGCGATTCCGCGCAAACCCGG14.545454545454546No Hit
GGGTGCGCAGGATCTGCTGGAACTGCTGCTGCTTGCTGTCGCCACGGTCT14.545454545454546No Hit
AAGCGATGAACAGGCTGATGGCGCTGATTGAGTCCGCCAGCAGGTTCTCG14.545454545454546No Hit
CCAGCAACCCGAGCGCGCGCTCCTGCGCCATCGGCTCGAGCAGCAAGGTG14.545454545454546No Hit
GATCAGTATTGCATGTTCGATTTCCAATGCCGCCTCATCGAAGCGGCATC14.545454545454546No Hit
ATAGAGTTCATGGCATTAGAGGTTACTTTGACGAGGTTTGCGATGTCCCG14.545454545454546No Hit
CTTTTGATATTCATCCTGCGCCAGCGCTTCATTGAGCGCCGGCCACGCCA14.545454545454546No Hit
GCCTCGGCCTGCGCCGCGATCAGCGCCTTTTCCCGGTCCTGCAACAGGCT14.545454545454546No Hit
ATCAAGCTGGAAGTCGTCGAGCTGATGGATTCCGGTGCTGCCGAGAGTTC14.545454545454546No Hit
ACGCGGGCGCGCAATTCCACGACGTCGATCGCCTGATCGTAGATCTGTCT14.545454545454546No Hit
GAAAGTTCTGCCTCTCGCGCCCCAATCTCGTGGGGGCTGCGTCTGACAGA14.545454545454546No Hit
GGGGTGGCGAGATCGCTACCGCGCTGCTCGGCGGTCATATCCAGGTCGGC14.545454545454546No Hit
GCCCACTCAGCGGTTTTTGGCACGAATCCCGCAAAAAAGCGCGGTGACCG14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)