FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C13I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C13I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length251
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGACCTGATACAGCTCGGTCTCGCTCAGGTTGAACTGCTTGAGCAGG11.4492753623188406No Hit
GCGTTGAAGTCGCGGGTCGGGGTGAAGTAGTCGGTGGTGTGGAATTTCAC11.4492753623188406No Hit
AAAAGATACTGAGAAATCTTTCGGAACAGTGAAGATTAATCTGCTGGAGC11.4492753623188406No Hit
ATCCCTGCATGTCTTGCCGCAGCGATTCGATCTCGTATGCCGTCTTCTGC11.4492753623188406RNA PCR Primer, Index 42 (96% over 26bp)
ATATCGAGTCGAATGCAAATACGCACAAGATCAAAAGCCCCTCACCCTAG11.4492753623188406No Hit
GGAATTCATTCCGTTACCGGAAACGCCGGCGCCCAAACCCGTCAATTACC11.4492753623188406No Hit
ATTTTCCTGCAGCCGTTCTGCAACCTCCGTTTTTCGATGCCACAGCGGAT11.4492753623188406No Hit
CCTTTCAGTGGTTGGCTTGCTGTGTGGGAGCAATCTTGCGCCCGCCCATC11.4492753623188406No Hit
GTGTAGGCCATCGGCACGGGCGCGGCGCGAGACTTCGCGGGCTTCGTCTT11.4492753623188406No Hit
ACCGCGACCCGCGCAACATCGTCAGCCACTTTATCGGCATACCGCTGATC11.4492753623188406No Hit
CACTTCAGGGGCAAAGGTATCAGGCCCCTCGCTCTATTCAGGCGAACATT11.4492753623188406No Hit
TCACCAACATTGCCAATCCTACGGGCACCGGCAACCCGAACGCGGGGGAA11.4492753623188406No Hit
ATCATATACGGGGCGTCCCGCAGGAGACACCCCAGCGTTGTATGAAACAC11.4492753623188406No Hit
TCCTGTCTGAAAGCTACGAAAAGGGCGAGATCAACCAATCCTGTCTCTTA11.4492753623188406No Hit
CCATAAAGGGTATTCGCATGCATTGCCACATGAATCATTCGATAGACCAT11.4492753623188406No Hit
GCTTGAGGACGTTGAACTCGACGCCGTAGAGCTTGCCCTTGTCGCCGTTG11.4492753623188406No Hit
GTGCGCGCCGGCGACCAGGCGCGGCATCATGAACAACAACGCCAAGCCCC11.4492753623188406No Hit
ATTACCAACAGGTGCTTTCATGCATTTTCCCGAACTGACCATTTCCCGTC11.4492753623188406No Hit
GTTCTCGACGTTGGCGCTGAGCCCGTAGGTCACGGAGGCACGAACGGTGT11.4492753623188406No Hit
CTTCCTGCACGACGTCGTCCATCACCTGCACGACGTCGACGGCTAGCAGG11.4492753623188406No Hit
GATCTGGGTACGCCCGGCGCGCACGGCTTCTTCAGCCTGATCGGCAACAT11.4492753623188406No Hit
CACAAAATTGCTCGCGGCTGGGCGCCCGGGTCAATCCCGGATGACCGATG11.4492753623188406No Hit
GAGAATCACCACATGGCCTTCGCGCTGTTCTTCATGCGCCGCCGATGCCA11.4492753623188406No Hit
ACGTTCGACCCTCGGCGGCACCTGTCTGAACGTCGGTTGCATGCCGTCCA11.4492753623188406No Hit
CGTCAGGTGAGAGGCCCGCCATCTCCCCGGCCGCCATCGCCGTGTAGGTG11.4492753623188406No Hit
GTACTCTAACCGCCAAGTGTACCGATTTCTAAAATGCGCTTTGCACCCTT11.4492753623188406No Hit
TCAATGCATCGGTGAAAATCTCGGGCGAGGTATCTCCGTGCCAGGACAAC11.4492753623188406No Hit
GAATAAAGAGAGTCAGAATGAAAATGAATAAAAAAGATGCTGACCAGAAA11.4492753623188406No Hit
ATACAGCACGCACCAACGTTGCCGACCTCAGTCGAGGCAATCTGGGCGTG11.4492753623188406No Hit
GGTCTGAACATCCTCGGCGTGAAACTCGCCGCCACCTTCGAGTTGGTGGT11.4492753623188406No Hit
GAATGATGTCTCCGCTTTTCATTCGATCACTTACATTATGAACAATCTGG11.4492753623188406No Hit
CCAGCGGCGCGCGCAGTGGGCCGTTGCGCGCCTTGTCGATCATCGCGTCG11.4492753623188406No Hit
CTATACAACATATGCATTTTTTTGTCCACGTATTAATTTATTTTGAGAAA11.4492753623188406No Hit
CGGTGATAAAGCCTTGTTCCAGATCGGCCTGGTAGCAGATGAACAGCAGG11.4492753623188406No Hit
GGCCTGTACACCCCGGCCAACAGCATCTCCAACGAAGAGCTGGTGCAGTC11.4492753623188406No Hit
CTTGTACACCACCAGGCCGGTGACCAGCGATGCCAGCAGCGGCAAGCCGA11.4492753623188406No Hit
ACTTTCGATCCTCTCGGGCGACGCCGGAGAGGGAAACCGCGCACTGCGCG11.4492753623188406No Hit
CCTTAAAACAAGTGAGGTCCAAGTTATTCCGAAATATATGTTGAAAAAGA11.4492753623188406No Hit
TCTTTCTGCTAACCATCCAAAGAAAATAGCACCAATCGCTTGTCCGATAT11.4492753623188406No Hit
TTTCAAGAACTGTATATCCGGAAACAGCAATAACTACCGCAATTGCGAGG11.4492753623188406No Hit
CTGTTGTGGATGGCAAGAAGGTCCGCGTGGCCAAGCGTTCGGGAGAAGTG11.4492753623188406No Hit
AGCCTGCTGTCGAGTGAGAAGCTGATCAGCGGCGACAAGTACACCTTCAT11.4492753623188406No Hit
GCTTCAATGAGCTGCTGAATACGGGCTTGTACAGCAACACGGGAAAGGCC11.4492753623188406No Hit
CTTCATGGGCGAGGAGAATCGGCTCGCACTCGGCTTCGAACGCCTGGGTC11.4492753623188406No Hit
CTGTTATGTGAGCAACAGATCTGTCGATAAGATCTCGGTAATAGACTTGC11.4492753623188406No Hit
GTTGATTTCCACCGGGTCTTCGGCGGTGGAGATGTTGATGTCGACGGTGT11.4492753623188406No Hit
ATCCTGCACGCATCTCGCAGTATAATTCGATTTTTTCATCCGGCTTTTCA11.4492753623188406No Hit
TCTCAGTGGCGATTTTCGAAACCTCCGCCATGTCATCCGGCAGGGCGGAA11.4492753623188406No Hit
GGAGATGCATCAGGGCATTGCCCGGAACCAGGCCAGCCCCGGCGATGTGC11.4492753623188406No Hit
GCTTTTTCACCGCGGCACTTCGCTGGGGCGCTTGTATTCCATCTGTCTCT11.4492753623188406No Hit
CATCCCGCGTGCGGTAATAATCGTAGAAACTGCCGCCGTTGAGCATCTGA11.4492753623188406No Hit
TCTTCGGGCAGCACCGGCAGCAGGCGTGGGTCCTGGGCGGCGAGGAAGCA11.4492753623188406No Hit
GGACCAGGGCGCCATGGGCCGTTTTCTGTTGGAGTCAGGGTTGATTGCCT11.4492753623188406No Hit
GGTCACGCCGGAAGGCGCGGGCGGGCCGACTTACCCGAATATGTAAGCCA11.4492753623188406No Hit
CCACCTTTGCCGATGAGCGGCCCGATCTGGTGGCGGAACTGGTGGCTACG11.4492753623188406No Hit
ACAAAACGCCGCAGAACCGAAAGGCTCTGCGGCGTTTTGTTGTGTGCGGT11.4492753623188406No Hit
GTCAGATCGTAGGCAGCAAGAATTTCCATGATTTCTCCGTCAGACTTCAT11.4492753623188406No Hit
AAATTGCCAAGCTTCACAAGATTTTGTGGATCAGCAGTGATGATTCCAGA11.4492753623188406No Hit
ATATAACTGTGGCCGGTGACCCAGCCGACGTCGGCGGTGCACCAGAACAC11.4492753623188406No Hit
CTCCACGACCCCGACGGCTCGGCCGTCTGGGACGGCGAGTTCCGCATCGT11.4492753623188406No Hit
TCCCTAAGCGGTCTCCGCCTCCGATGATTGTTACAATCGTTGTCAAACCT11.4492753623188406No Hit
GGACTAATGCGGTGGCGGCTTCCAGATCATTCTTCACCGAGTTTGCTTTA11.4492753623188406No Hit
GCTCCCTCTCCCGAGGGAGAGGGAGCCGATCGAGATGTCTGGCGTTGAGC11.4492753623188406No Hit
GCTTCGAGCAGGCGCTGGGCATCGACTTGCTGCTCGCGCAGAACGCGGGC11.4492753623188406No Hit
CCCAGATTTCGGTGCGAGTGGCGGCGGGCAGGACGGCCTGGATGTACACG11.4492753623188406No Hit
ATCGCGCTCCATCCACAGCAGTTCCCAGTGGAAGAACACGTAGCGTTTCC11.4492753623188406No Hit
CCGTAGGATTCCGACCAGCCCGGCGTGACGGTGAGAATGCGCTTCCTGAG11.4492753623188406No Hit
TTCATGTACATGGGCAAACTGGTGGAATTCGGCGATACCGATACCCTGTT11.4492753623188406No Hit
TTCCTGTGGGGCCTGTTCAATTCGATGTACCTGCTGATGGCGCTGTCGAT11.4492753623188406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)