FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C12I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C12I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length251
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGTCAAGCACTTATTGGAATGGCAATGCTTGTTTCAGGGGTCGTACTA11.5384615384615385No Hit
TCACTTGCAAGTTTCCCGTGCATTAAGCCGATCGACCATTTTCCGCGATA11.5384615384615385No Hit
CCGTCATCCTGAGAAGTTTCGACGCCGTGATTTATTGGTTTATTTTATCG11.5384615384615385No Hit
AGATGAAAGCGTCAGGGTACTGGTTCAAAACATTAATCCAAACGAGGAAA11.5384615384615385No Hit
TATTAAAACAAGTAGGAGCCCCGCGTTTTTGTACAGCGCTAAAGACCGTA11.5384615384615385No Hit
GCACCGTCTTGAGCCTGCGTTTGACTTCACGCTCGTTCTTGCTTCCAAAA11.5384615384615385No Hit
CGTCAAGGGCGGTAAGCGCTTCGGCTTCGCCGCACTCGTGGTCGTCGGTG11.5384615384615385No Hit
GATTAACATGAAAAAACATTGACGCCTTGACGGAGGCGCAGTACAATATC11.5384615384615385No Hit
GTTTATTAACAGTATGTATTGGAACAATTTTTGGCATTTTAATTGGTGGT11.5384615384615385No Hit
TAATAGTTCGGTGCGGAAATTGTTCAAATCAGAAGCCGCATCGTCTTTCA11.5384615384615385No Hit
ATGTATCGCGGGCCGGGGTGTGTAGGAAAATGGTTTTTGGAGGCCCGGAA11.5384615384615385No Hit
CATCATTCACCTCGTGATTGGGCAGTACCGTCCGGGAAGTGACGGGCAAT11.5384615384615385No Hit
GTAATTCGTCTACATCGAAAACAGTGGGGGGAAAATCGCTTTTTGTATGA11.5384615384615385No Hit
CTCCACCCGAAGGCAGCCGGAACTCGAAATCGGGCAGATGGTCGCGCAGC11.5384615384615385No Hit
GCTATGGAGAAGACGCTAAAACGAGGTGGAAGAAAATGGCCCAGCTGTAT11.5384615384615385No Hit
GTCTTGGGGAGAATGTAAAAAATCAGGCAAACACCATTTGTGCCCACATG11.5384615384615385No Hit
GATTATGTTTTTTCTGCACAATCTGATACTGTCAAAGTCTCTTCTGGAGA11.5384615384615385No Hit
ATGCTGAACGGATGATTGGTCGAGTACTGCATCCAGAAAATCCAGCGCCC11.5384615384615385No Hit
GCACCCCGACCTCGCGCGACGTCCCGCGTTCGCCGTTCCAGACGAAGACG11.5384615384615385No Hit
ACCCCACGCTGATGGCGGCGGACATCCTGCTCTACCAGAGCGACCTGGTG11.5384615384615385No Hit
GGATGCCGTCGGCCGGAACCATGGCCTCGTCGATACCGACCTGACCGGAC11.5384615384615385No Hit
CGCAAAGGTAACATCCTGAAATTGGACGTGTCCCTCTAGTTTCTTCTGAA11.5384615384615385No Hit
GCCGATGATTGTCTCGATATGCTGGACGATCTGGCGACGCGACCGGAAAC11.5384615384615385No Hit
TCGTCTCAATTGGCTCTACTAGCACAATTCCTTTTTCCACAAAGAAATCA11.5384615384615385No Hit
GGCTTCCACCACCGAAAGTGACGCCTTCTCCTTTTTGAACTCGTCCGATA11.5384615384615385No Hit
CATAAAAGTTTTCACTCATTATATCAATCCTTGTATGAAATAGGGAGCAA11.5384615384615385No Hit
ATAGTACATGTATGCTTTGATTGCTGATGAAAGGCGTTTATCGCTGCCTC11.5384615384615385No Hit
GTATAGTATGCTTATCAAACTAGAATTGAAGCAAATGCTCAGAAGTCGTT11.5384615384615385No Hit
GGATAGCATTGCCCATTTCAGCCAAAATGGAAAGCCTATAACAGAAGAAG11.5384615384615385No Hit
ATTATGTCCGCATAAAAAGAGTGGAAACAAGAATCATAGATCAAGGTGTA11.5384615384615385No Hit
TATTTAAATTGACCAATCGAGAGATTCTAAGAATGCATATACATCCTGAT11.5384615384615385No Hit
ACACAGTGGAAGTTCGTAAAACTTTAGGAATGGATAATCCATGGAATTAT11.5384615384615385No Hit
ATTTTGGGTTCATCATTGAAAGTGATAAAATAGAGCTGTATTTCTGAAAC11.5384615384615385No Hit
GGGCTGATGGCTTGAGGATGACGGCGTTTCCTCCCGCGATGGCGGGACCC11.5384615384615385No Hit
CCTCGAACCTGCGCGGTGAGTTAATTTCGTCCTGTCACTGTTGGCCCTGT11.5384615384615385No Hit
ATCGAAATTTCTTATCGGTTTCTCTCACACTTTCGCGAATCTTCATACTG11.5384615384615385No Hit
ATGACCGCCAGAACACCGCTGACGATCCCCATGCGGCGGCCGGCCGCCCG11.5384615384615385No Hit
GTTTTTATTAGATTTTTCCTGTGCGGTACTGGCAGCCTTACTCGACTTAT11.5384615384615385No Hit
TTTCTAAATCTGTTAATGTTTTACTTCCATAAACTCCTGGCTCTCTCTTG11.5384615384615385No Hit
GTTGAAGGTGGTGGACTTGCCCGCGCCGTTGGGGCCGATGAGCGCGAGCA11.5384615384615385No Hit
GCCAATTTCCCATCCTCTAATATGCGCCTTGCTTCGGTCTTCTCATTTTG11.5384615384615385No Hit
GTAGTCGGACCGACCTCCTTCACCACGCGGCGCACCAGCGCCTCCTCCCC11.5384615384615385No Hit
GTTATATTATCTCTCAATTCTCCATTTTCTGAAATAGTCGAACATGCTTA11.5384615384615385No Hit
GTATTTTTGCGCTCAGCTCAGATTTTCCAGTGTTTGCGTGTCCCAACTGT11.5384615384615385No Hit
AGCATGGAGTGCACAAAACCGCAGCCCATTGATTCGTATTCCAGCATCAC11.5384615384615385No Hit
CATCTCTGCCACAACAAACGGCATGGTTGTCACAATCGCTCAGCGTCTGA11.5384615384615385No Hit
TTACGAAGCATGTCATGTGTTTCATCAACCACTTGTCTGACTGTTTCAGA11.5384615384615385No Hit
CAATCAAATCAAAGAGCTTTCACGTGAAAGTTCCGCAGGTTTCCAAGATA11.5384615384615385No Hit
ATCATAATCAAACCAGTTGACTTTATAGATTATGATCATCATCATTAAAT11.5384615384615385No Hit
ACTCAAGATAGAGCGGCAGGTGGTCCGGCAGCTCGCGGCTATCGAGCTGC11.5384615384615385No Hit
GGCAAATACGAATCGGAATATCAAAGGTTTGACAAATCTCCTCACTCCCT11.5384615384615385No Hit
TTATTACATATGACCAGCATGTGATTCGTCATGATACGTTGAAAGTAGCT11.5384615384615385No Hit
GACCATTTCCGTCTACACCCGCAATTCCGACAATCTCACCAGCTTTCACT11.5384615384615385No Hit
GCTCTATATAATCTAGAAGAGACGGGAGAATACGCGCCCGAAATTTAGAT11.5384615384615385No Hit
GTTCCATAGTAAAGGATACATGACCCCAAAGACGATGCCTGCCAGAATAC11.5384615384615385No Hit
TTATCATGTATGAATGACCGTAACAAATGACCAAAAACAACTAGAAAAAT11.5384615384615385No Hit
CGTCTATCACATGAACAACATCACTCTAAAAGCTTGATAAGACCTTTTAT11.5384615384615385No Hit
ATCCAATGGATCGACCATTTTCATCACCTGCTTCTCTCTTTGTGTGATTA11.5384615384615385No Hit
GACCTGAATGTACCTACCGTCCTTGCCTCTCAACTTTCGACGCTGCGGTA11.5384615384615385No Hit
ATAACACACCGATTTTCTTTTGAATAAAGGCAGGGGTCAGCTTGTCCAGC11.5384615384615385No Hit
ATGAAAGAGACGGCTATGAGATAGAGTTTCATGAAAAAGGGGGAAGTGAC11.5384615384615385No Hit
CCTAAAAAAGATAGACGTATACACGTCTATCTCAAATTGTTGACAAAAGG11.5384615384615385No Hit
TTCTTGAGCAGCGTGTGGAAGGCCTGCTGTACGCCGCAACACACCACCGC11.5384615384615385No Hit
GAATCAGAAGTGGGTCAAGGCGGAAAGTTAGGCGCTCTAGATTATACGAT11.5384615384615385No Hit
GGCTCCTGAGCTTCATCCTTCCCGCAGTTTAATCAAAGGAGTCATCTGTG11.5384615384615385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)