FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C10I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C10I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGTACACACCATAAGCCAGCTCTCGAGCTGGCTTATGCGTATTCAAGA11.2345679012345678No Hit
CTGTTGTTCAAGCTGTTGCGCACATCGAAGGTGAGGTTGTTCTGGCCGTA11.2345679012345678No Hit
GTTTTTCTTCAGGTAATTGCCGACCAGTTCGCGGATCTCGCGGTCGTCGT11.2345679012345678No Hit
CCGCGAATGCGTGCGCCGAAACCGCTTTGTTGGCGGAATCAATCAGTGCC11.2345679012345678No Hit
CAGGTTTTCAACGAACTCCCAGTCGTCACCCTGATTCCATGGGCCGCGGG11.2345679012345678No Hit
GCTTCTAGAAAACCTGCGCAGCGCCTGCCATCGAACGTCACTTGCGCCAG11.2345679012345678No Hit
CCCTTCCAGGGGGCGGACTGGATCTGGCTGTGCAGGGTGCCGGCGCGGCT11.2345679012345678No Hit
GCCGCGCAGAGTGCCGTGACGCCGCGGGAAGGCTTGCTCGCGATGGTCTC11.2345679012345678No Hit
TCTTCGCCATCGCCCGCCTGGTGCCTGCACTACTCCTGGCCCCTGTGCCG11.2345679012345678No Hit
TAGTCCCGCAGGCAGGTTCCGTCCGGGGTGTCGTAGTCACCGCCGAACAC11.2345679012345678No Hit
GGTAGTAGGCGTGCTCCCACACGTCGAGACCGAGGATCGGCGTATTGCCG11.2345679012345678No Hit
TGGTTAGCCAATGCTCGTTCCCTTCCTGATCATGCTGCGCGAAGGCCTGC11.2345679012345678No Hit
GCACATTCAAGCCCAGCCCGATCACCGCGCGCGCATTGCCGGCCATCTCA11.2345679012345678No Hit
AACCGGTACCCGCCCCAACGACGGTTTCGCCCACGCCGGTTCCGACGACG11.2345679012345678No Hit
GCTTATTTCTCGCCCTTGAGCCAGGTGCTCAAGCGCGAATTGCTCTACGT11.2345679012345678No Hit
ACGGTGAGGTGCGATGCCGCCCACCGGTATCGTCGCTTACTCGGTGAATG11.2345679012345678No Hit
TCTGCAGACCGGCCACCAGGCCTTCGATTTCCTCGGTGGATTTCTGCGTG11.2345679012345678No Hit
GCTTTTGCTCGTTGGCATCTGCAGACTGAACGAAAACCTTGGCGTCCATT11.2345679012345678No Hit
CCGTAATCCACCACTATGGGCACGAGACCGAGCGACATCTCGTATGCCGT11.2345679012345678No Hit
GGCTGATAGGCCTGCGGGTCAACGGGATGGCGGATGCGCCAGCACGCCTG11.2345679012345678No Hit
CGCCAAGAAGATGGTCGAGGACATCAACAAGGCGCTGGCGGAGTAAGATC11.2345679012345678No Hit
GTCCTGTACGTTGCAGGATCGGTAATACCCCCTCAACAGAGAAGAGATGC11.2345679012345678No Hit
GAACCAGAATACGGGGGCGATAGCGAGACGCCACCAGAGTGCCCATGTCG11.2345679012345678No Hit
CGTTGACGCAGACGGCATCGATGTCGCTCGCGATCGCCAGCGTGCGCGGC11.2345679012345678No Hit
GGCAGTGCCCGGCTGGTGGTGGGCGACAAGGTCATGCCTGTCTCTTATAC11.2345679012345678No Hit
GGCCATAAGTGCGCACGGCTGCATGGGCATTCGTTCCGGGTCGAGCTGCA11.2345679012345678No Hit
GACGTGATGAACTTCGGCATGGCCAGCGAACGGCTGGCCGCCGAGGGCAT11.2345679012345678No Hit
GCCAAGGTGGGCATCGCCATCGATCCCAACTTCAAGAATTCCGACGGCCA11.2345679012345678No Hit
CTGTTTGTCCAGCACGATGCCGCCGAAGTGGTCCTTGACCTCGAACTGCT11.2345679012345678No Hit
GATATGGTCCGATTTGAAGTTTCCTCGAGAATCCCCATATCGGGCTCATG11.2345679012345678No Hit
GCGCAGGTGTTTGCGCGCTTGGATCAGTTGCCGATGCAGGGCAAGAGCAC11.2345679012345678No Hit
GTTTTCGGAGGCCACCTGTGCTGCCGCAAGCCGGCGGGCCGCCCGGGGAT11.2345679012345678No Hit
GATAGGTCACGGTGGTTTTCTTCAGGTGCCACTGATTGGCATGGATCGCT11.2345679012345678No Hit
CAGGGGATCTGTGCGCCCGCCTTGTCCTTACCCACGAACTCGCGCAGGAA11.2345679012345678No Hit
CCTACCACAGTGAGACCCTGCCCGACTCCGGACTCAAGCCCAACGTCCTG11.2345679012345678No Hit
GTTGGTGGCGGCGATGATGTTCACCGTGGTGGGGCAATTGATTATCGCCA11.2345679012345678No Hit
CACAAGGCATCAGCGCCCCGGGTAAGCGCCTGGAGGGCCTCTTCACGGCC11.2345679012345678No Hit
GTCTGCAGTTGCTGCTGGGTTTCCTTGAGCTGGGTGATGTCGCGCACCAC11.2345679012345678No Hit
TTGTCAGCTCGTGGCTCATCAAGTGTCTCCGGCTCTGTAGCAAGTCAGTT11.2345679012345678No Hit
AGACTGTGTGTCGAGCGCCGTGAACCTGCTGGTCCGGTAGTAGATGGCCA11.2345679012345678No Hit
ACGTTGTAGACCGTCTTGGGCGTTACGGGCGCCTTGATCTTGCCGAACTC11.2345679012345678No Hit
GCCGGAGAGTGTGTAAGGCAGCACCTTGGCGCCAGGGTCCTCCTTGTGCA11.2345679012345678No Hit
GGCCGATTACGTCTGGTTCACACCGGCGACGCCGGCCAGGGATTACTGCG11.2345679012345678No Hit
CTGCAAGGCCAGGCCGCACAGGCCGAGGCCGAGCGCGACCGGCATTGCCA11.2345679012345678No Hit
CATTCATTGTTCTCTGAAAAAACTGAGTATCGAATCTGCCATTAACTGGC11.2345679012345678No Hit
GCGATGACCGCCTCGATCAGATTGACCGAGCCGATGAATTCGGCTGCGAA11.2345679012345678No Hit
ATGCATGGCTTTTCTCGCCGATTCCCGCCGAACGGCGTACTAGAGCGGTC11.2345679012345678No Hit
TTGTAGTTGCGCACCGAGGTGTACAGCTGACGCTGGGCGTCGAGCACGTC11.2345679012345678No Hit
GCGCCGGCACCCTGTTTTTGCCGATCAACGCGGGCCTGGGTGGCTTCTGG11.2345679012345678No Hit
CCCTTGCGTAGCCGCTGGGCGATCATTTTCTGGATAGTCTTCTGTTTAAA11.2345679012345678No Hit
CTCCTATCCTTGTGGCGTTGGCTGTCGTTGCCGGCCCGCACGTGCAGGAA11.2345679012345678No Hit
AATACATTATGTGGACCCCGGGAGCACTGCGCTCCTGCCAAGCACAATAC11.2345679012345678No Hit
CATAACGCTCGCCCCAGGCATCGAAGCCTTGGATGTCGACGAAGTCATCG11.2345679012345678No Hit
ACATCCGAGTTGGCAAGCAGGGTAACGCCGCCATGGATGTTGGCCTGTCT11.2345679012345678No Hit
ACCGTTCCCAAGCCCTGCCAAAACGGCTGGAAGGAGCCCAGGAAGGGCAC11.2345679012345678No Hit
GGCAAAGGCTTTGGCTTGCAGTTTCGGGTTGAGTTCGAAGACTTCGGTCT11.2345679012345678No Hit
GTGCTTGGCGTGGTTCGTGTGAAGCACAAGCCGGGCCAAAGGGTGTAATT11.2345679012345678No Hit
GTAGTGATTCGGGGCGGAAAGGCCAGAACCGTGTTCTGCTTTTATTCCGC11.2345679012345678No Hit
GCTTAGATGCTTTCAGCGGTTATCTTTCCCGAACATAGCTACCCGGCAAT11.2345679012345678No Hit
CGGAGCGAATGGCGGCGATCTGGCCGTCCATCATGCCCGAGGGGCCCAGC11.2345679012345678No Hit
TTGCTGGCTGGCAGGATGTCCCGCTACCATTTCTGGTCTTTATCCCCTTT11.2345679012345678No Hit
CCGTTAATAGGGCGTAATGTTGGCTTGGTCACGCGGCCGTGCCGGGGACC11.2345679012345678No Hit
CCATGATCGCGGTCCAGGGCGGCCTTGCACTGCGGTCCGTTGCGGAGTGC11.2345679012345678No Hit
CGGTCCAGGAGGCCGCCATGTATCGCCGAATTCTTCTGTTTACCTGTCTC11.2345679012345678No Hit
GTGATGCTGTTCAATGGCCTGATCTGGCCGCATCTGGCCTTTCAATGGGC11.2345679012345678No Hit
GGAGAACTCCGCGGTGTCCGCGGCGATGATCTTGGCGCGGGTCAGCGTGG11.2345679012345678No Hit
AACAATTACTGCCCTGGCCAGGAAAATAAAAAGCGGCCACCTGCGGTAAC11.2345679012345678No Hit
AATCCATTGATCAACACAGTGGCTGTTGTTGCGAGGCATGCGGCCAACAG11.2345679012345678No Hit
TGAAAGAAGACGGCCAACGCGCAGCCCATGCGCTGTCTCTTATACACATC11.2345679012345678No Hit
GCGCGAAGTCCATCGCGATACCCGCAGTTTCGCCACCGCCCTGCGCTCGG11.2345679012345678No Hit
GATGCTCTCCTTGATGGGCGGGGCGGTGCAGGTCGGGGCCCGGGTTGCGG11.2345679012345678No Hit
TTACAATGCCCCGCGCTGGCTAACCGATGTCAACATAGAGAAATAATCTC11.2345679012345678No Hit
ATTACATCCTCAGTGGCCAGGAAGATCACTTCAAAGACCCGACCACGAAA11.2345679012345678No Hit
AAGCACCACCAGCCGTTGAAGTCGGGGCGATGCACGCGGGCCAGGAAGTC11.2345679012345678No Hit
GATCAACAGAGACATTCCGAGTTCCTCGCAGGCATGACCGACTGGTATCG11.2345679012345678No Hit
GGCCCGGAATACCTGCCTCCGGGATTCCGGAAAACTGGCATGCACAAGGC11.2345679012345678No Hit
GTGAAATAGTTGGTGATCACCCGCAGGCGGGTGACGCCTTTGAGTTCACT11.2345679012345678No Hit
GTGTGATAACTGGGGTGGCAGAGAGGTCCATTTCCATTTCGAAAACCCCA11.2345679012345678No Hit
CATCCGGTCCGCGGCCCGGAACCGCTGAATCAGCGAAGTAAAGTCCGCGT11.2345679012345678No Hit
CTCATGGGTACGCCGCTGCGCAGCCAGTGCGACTGCCTGGGTTTCCAGGC11.2345679012345678No Hit
CGCTTGCTGTCCACGTCCACGCTGCGCAGGTGCACCAGGATATCCGGGAT11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)