FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n01_Tr4_C10I1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n01_Tr4_C10I1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length251
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAGCTCGAAGGCCCGCAGGACCAGGTGCAGGCGTTCATCCTGGACA11.7241379310344827No Hit
TCCCTGATCCGGACCGGCCAAGGACTACTTTGACTTCACCCGAGCCCACG11.7241379310344827No Hit
GGCTGGATGTGGCCTGTGCGGCGGCACTGGCTACAAGGATCACGCGGGCC11.7241379310344827No Hit
TCCTTGGGGTCTGGGTGGCCTGGCTCGTAACCCGTCCGGCACCGGCCGGC11.7241379310344827No Hit
GCGTGACGGGAAGATGCCCGCCAAGTCGACGTGACGCAAACTGGTAGGCG11.7241379310344827No Hit
CGCGATTCACTGGATCAGGCAAGGCGAGCGCCGCGAACAGCGGGACGCTG11.7241379310344827No Hit
CAGCCCACCTCTGGGAAAAGGGGTCCCTGAGTAGGGAGCAAGGTCAGTCC11.7241379310344827No Hit
ATTCAAGAACATCGGTCTGCTTCGCGGTGTCCAAACGGCACAGGGCGGCG11.7241379310344827No Hit
TCGAAGGACTCCGCGGACGTGATCAGCGCGATGGACCCCACCGACGCGAG11.7241379310344827No Hit
AAGGTAAATCGTCGGATCTACCATCGATACGTTGTTAACTATCGTCCCGC11.7241379310344827No Hit
GGTCAAGATGGGCCAGCGCGAAGGCCAGCAGGTCTTCACGCAGATCGCCG11.7241379310344827No Hit
GTGGGCACGCTGCATGCCGAAAAACGCCGTTACGCCAGTGGCGGCCAAAC11.7241379310344827No Hit
GATCCAGACTTCGACGGCGGTTTCTATTGCTCGAAGGAAGCCGTCGAGCA11.7241379310344827No Hit
GCCTAGTTCCTTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCC11.7241379310344827No Hit
GTGGGACGCCACGAGAGGCGCAGCCGCGCACTGGCCATGCTGGACACCGT11.7241379310344827No Hit
TAGATGCCCTTCCAGCTGTCGGAGGCCATCGCCATGTCGACGCCGGCAAT11.7241379310344827No Hit
ACTCCAACCACAACCTGCATGAAGCGCTGGAAGTGCTGGACGAACGTAGC11.7241379310344827No Hit
CCCAGCGTCTACCCGGACCTGACCGTCGAAGCGAATGTCCGCTATTTCGG11.7241379310344827No Hit
CCTCGCCCTGGTCGACAAGGCGCAGATCCCGGCTGACGCCGCCGCTCGCA11.7241379310344827No Hit
GTCTTGAGGCGTTCCTGCGGTTTCTGGATGCCGACCAGCTCGAGCAGTTC11.7241379310344827No Hit
TCACCGGCCAGGGTCGCCAGAACGCGTTTTGAAGCTTCGTAACTATCCAT11.7241379310344827No Hit
GGATAAACATCAGCCATGGCACCCTCACTTGCGATCAACTTGTCGGAAGA11.7241379310344827No Hit
ACGTAGCACCGTAGGAGCGCGCTCACGCGCGATGAAGCTTTACCAGGAAC11.7241379310344827No Hit
AATTGATGGCGCGCGTCTCCGGCACCCTGGGCGGCATGTTGCTGCTGGTG11.7241379310344827No Hit
CTCTTCCTGGTCTCCCGGATGGACTGGCCCATCCGGGAGACCAGGAAGAG11.7241379310344827No Hit
GATCGCCTCCTTCACCGAGTAGTAGTCCGGCACGATTTCCTTGGTGGCGC11.7241379310344827No Hit
TGCACCACCGAACCGGCATCATCGGTGACGGTGAACGTCATCCCGTTCGT11.7241379310344827No Hit
CCATAGAGTTGGATGCCCGAAGTGGTGCGCGCATAAGGCACGCATTCGAG11.7241379310344827No Hit
ACCTGTTGCCGACCCAGACCCTGGTCGGCGCAGCCGTCGCCCGTGAACTC11.7241379310344827No Hit
TTGTGATTCCGGTCGAACTGGGCCTGCTGGACAGTCAGGGCAAAGATATT11.7241379310344827No Hit
TCCTATTTCCACGATGACCATGTGGGGACGCATAGCTCAGTTGGTAGAGC11.7241379310344827No Hit
ATCCTGCGACTTGCCGGGATGGCTGGTCCAGAAGTCGAACGATCCTGCGG11.7241379310344827No Hit
GAACAGCGTGATCCCGGACACCGCCAGTTGGCGTAGGGCATCAACATCGC11.7241379310344827No Hit
GACTGCTCCCCTACGCCGCCGGGGCCGCTGAACCTTTCCTCGGAGCTCAT11.7241379310344827No Hit
GCACTGGCCCGTGGTGAAGCTCAGCGACTTGGCCCAGCCGGTGCCAAGGC11.7241379310344827No Hit
GGATACGGGCCTGCGCGGCGATGACCAGCCCCCTGCAGGTGGCCGCGGGC11.7241379310344827No Hit
GCCCTGGGCATTCCGATCACCACCTACGTCAACATGATCTCCGGCCCGCG11.7241379310344827No Hit
AGGCTAAGGGGCAGGAGAGGCTGGCGCGCAGTTTTTTGCCGCCAGATGAA11.7241379310344827No Hit
GTGCGTGTCCTATTCCGATGGTGCGTGCCCGCTTGAGAATCCGGTTGCAG11.7241379310344827No Hit
CTGCTGCAGGGCATGGGAGTAATTTTCGGTGAAGCCTTCGAGCAGGAGCC11.7241379310344827No Hit
CCCTGGGTGCATCCGCCGTCCTCGCCCCTGCCTCCCTGCGCGTGCACCAG11.7241379310344827No Hit
TTCGACGAGAGCTTTGCATTTCGCGATTCGCGCGAGCGCTGGCTCTATAC11.7241379310344827No Hit
CCCTGTGGGAGCGGGCTTGCCCGCGAAGGCGTCGGCACATTCAACATCTC11.7241379310344827No Hit
ATGTGCGCATCACCGCCACCGGAGTCAGGTCTGGGGCTGAAGGGCGGGAT11.7241379310344827No Hit
TGTCCGGGTTGTGGTGAAGGAGGCCGAAGTGACACTCGACGGCACGGTGT11.7241379310344827No Hit
CACCAGCCCGTTGCCTTCCTCTGCGATGCGCGAGATCAGCTTGCCCTGAG11.7241379310344827No Hit
ACAGCCAGCTTCGTGTGCCGCACCGAGTCCCCACTCCCCGTGGAGCAGCT11.7241379310344827No Hit
GGTTCATGGTCATGCTGGCAGCGAGCTTCTTCAGCTGGCCTTTCGGCAGG11.7241379310344827No Hit
GACTACACCGTGCATGACAACACCTACGAAAACGTCCGCAAGGCGGCATC11.7241379310344827No Hit
GTGGACATGTCCGCGCTGCCCAAGGTTTCCGCCGAGGACATGTCCGACCA11.7241379310344827No Hit
GATTGGTGAGCGGCGACAGGAAGCTGGACAGCAGATAACCGTGGGCGCAG11.7241379310344827No Hit
GCATGGGCGCATCACGCAGCACCTGGTCGAGCGCAAGCAGCGCCATGCCC11.7241379310344827No Hit
GGACTTGAACGCCATGTAGGCCGCGTTCGATTTCGGCGCGGTCGCCACGT11.7241379310344827No Hit
AATTTACGCGAAATTCATTTCAAATATGTTTCATTAAAAATAAGTTTTTT11.7241379310344827No Hit
TCGTTATACACAAGGCATCTGGCTATCTGGAGAGGCAGCGGTACTGATGT11.7241379310344827No Hit
ATGGAATGGGGCGAGATGCTCAAGCGCGCCAAGGCCGGCGAACACGATAT11.7241379310344827No Hit
GGTTTCTCCACTGCCCCCTGCTAGCCGTCGCCGACGCACTCCTTTTCGAA11.7241379310344827No Hit
CCGAGCGGCCCAGCAGTGTCTGCGCCACGGCGTCCGGCTGGTCATGGATT11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph