FastQCFastQC Report
Wed 21 Dec 2022
000000000-KDH3G_l01_n01_Top10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KDH3G_l01_n01_Top10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8226310
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCACCACCCTGAC245530.2984691799847076No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCACCACCCTGA216410.26307056262163714No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACCACCCTGACCT200690.24396114418250708No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTC197500.2400833423491213No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCACCACCCTGACC184950.22482741350617713No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGT171790.20882996142863566No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC149530.18177044142513474No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT148020.17993486751654145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)