FastQCFastQC Report
Sat 10 Sep 2022
000000000-KBYBL_l01_n01_ORFSub6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KBYBL_l01_n01_ORFSub6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066074
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT49552746.481482523727244No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA47035644.12038939135557No Hit
ATCGATTCCTTGGTTCTTGCGGAAAGGACGAAACACCGCCAGTAGGTCCA131701.2353739046257577No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCACTA21500.20167455542485793No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCAC19290.18094428716955857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)