FastQCFastQC Report
Sat 10 Sep 2022
000000000-KBYBL_l01_n01_ORFSub2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KBYBL_l01_n01_ORFSub2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133287
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC52581646.39742624772013No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA51331145.29399878406794No Hit
GATCGCGCGGTTCTTGTGGTAAGGACGAAACACCGCCAGTAGGTCCACTA54170.4779901295964747No Hit
TCGATCGTTACCATCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCACT20040.1768307586692515No Hit
GATCGCGCGGTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCACTAT17720.15635933351392894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)