FastQCFastQC Report
Sat 10 Sep 2022
000000000-KBYBL_l01_n01_ORFSub1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KBYBL_l01_n01_ORFSub1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198755
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA59942350.00379560460645No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC50075341.772755900913864No Hit
GATCGCGCGGTTCTTGTGGTAAGGACGAAACACCGCCAGTAGGTCCACTA59490.4962648748076129No Hit
GATCGCGCGGTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCACTAT20800.17351335343752478No Hit
TCGATCGTTACCATCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCACT18900.15766357596005856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)