FastQCFastQC Report
Wed 16 Mar 2022
000000000-KB2N6_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KB2N6_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27089898
Sequences flagged as poor quality0
Sequence length8
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAGACG1709076063.089052605513686No Hit
GATATCGA886265232.71570826881666No Hit
TCTTTCCC4259771.572457009620339Illumina Single End PCR Primer 1 (100% over 8bp)
GATATCCA566900.20926619952574202No Hit
CGCATACG559070.20637582319431397No Hit
CGCAGCCG556920.20558216941237653No Hit
GACATCGA297650.10987490613659748No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)