FastQCFastQC Report
Wed 16 Mar 2022
000000000-KB2N6_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KB2N6_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27089898
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTAC1683770862.15493317841212No Hit
AGGCAGAA870797032.144713132548524No Hit
CTCTCTCC2248080.8298591600455639No Hit
CTCTCTAA712520.2630205547470131No Hit
TCTCTACA537340.1983543828773368No Hit
CTCTCTAT407310.15035494042834713ABI Solid3 Adapter B (100% over 8bp)
AGGCAGAC331310.12230020209009276No Hit
CTCCCTAC310750.11471065708700712No Hit
AGGCAGCA279780.10327835121416848No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)