FastQCFastQC Report
Fri 29 Apr 2022
000000000-K6NVC_l01_n02_FC_PCR_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K6NVC_l01_n02_FC_PCR_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAAGTGGCACATACTAGGCCAAGAGCCACTTCTGTGGTCACTGTT15.555555555555555No Hit
GCCGATACTCTTCCCTCATAGACTCAGGCACTCCTTCCGTAGAAGGCCCT15.555555555555555No Hit
GCCTGCTTGTGTGTACGGGCTCGCTGTGGCCAGTGGATATGATTTTGAGA15.555555555555555No Hit
TTGCAAGCATGAGGATTTACTACTTCACGGTGGAAGTGTCTCATTGCAGG15.555555555555555No Hit
GTGCATGTGTCTAGAATGAGGGGATGTCCCAGGCTTGTTGCACACAGCAT15.555555555555555No Hit
TAATAAGACTGAAGACCTGACTGTTCTGAAGCAGACGGAAAGGATCTATC15.555555555555555No Hit
GCCTCATATCAAGTGCACTCTCCCACTGATACTCTCTGCAACAACGAGAG15.555555555555555No Hit
GTCAAGCAGGACCCATGTTCTCCTTCTCCTCTCCTTCCGAGTGCATCCCC15.555555555555555No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG15.555555555555555No Hit
CCACCAACCCACTAATCAGGCATGAGAACAGAATGGTACTAGCCAGCACT15.555555555555555No Hit
CACATCCAGATGCTACAGACGTCACACACAACCTCTTACTAGAAGATCAT15.555555555555555No Hit
AGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTT15.555555555555555No Hit
ACTAATAATAATCAATTCCTCCCCGCAGACCATTCACAAGGGTCCTTGGC15.555555555555555No Hit
TTTTGATATAGCACGGGTCCATACCAGCCCTTGCAATTGTCTCCGCAAAC15.555555555555555No Hit
CTCTTCAATGGCGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT15.555555555555555No Hit
ATGATGGATCAGGTGCGAGAAAGCAGAAATCCTGGGAATGCTGACGTTGA15.555555555555555No Hit
CCCCACCCCCCCCCCCACCCCCCCCCCCCCCTCCCCCCCCCCCCTCCCAC15.555555555555555No Hit
GTCTCCAAAGCGACGACCACCTCATCTGAAGAACCTACCATCAAGAACTC15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)