FastQCFastQC Report
Mon 2 May 2022
000000000-K6NVC_l01_n02_FCC_Seq_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K6NVC_l01_n02_FCC_Seq_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGAAATACTGATACCCGACCAATCCTCCCTATCCCCTTCTCGTACCT11.8181818181818181No Hit
GTTTAGTTTCAACCTCACTGAATTCATGATCAATTATTTCATATTGCTTG11.8181818181818181No Hit
GCTTTATCAACGCATTCGTTAGCGGTTAAAACATGGTTCGCACAATTATT11.8181818181818181No Hit
CTCCATTACCCGATACTGTCTGGGCGGGATAGGAGATCGGACCATCTCAA11.8181818181818181No Hit
TAAGAAAACTGTGCCTCTGGGGTGCCTGGGTGGCTCAGTGGGTTAAGCCG11.8181818181818181No Hit
GAAGCGACTCTTCCTTCCTCGTCTCCGGAACCTCTGCTGTCAAAGGCACT11.8181818181818181No Hit
GTGTTTTGCAGGCATTCAAGCCCTCCAATGCGGACCCGAGCTGCAATTTG11.8181818181818181No Hit
TCACTCCCCATGCCTGCAGATTCCTTCAATGGTCCAACATATCTTCATGG11.8181818181818181No Hit
GACTCCAGGAGCTTGAGCATCGCGCTCAAAAGAAGGTTAAATTTTATGGA11.8181818181818181No Hit
TTCCTGACATCATCCGGCGCCCAGAGCTACAGCAGGAGGACGCCAACATA11.8181818181818181No Hit
GTAGCAGAACTATAAACACTAAAAAATAGCTATACACCACACCTCTCAGG11.8181818181818181No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAGAAAAGACG11.8181818181818181No Hit
GCTCTTAGTTGTCTTGTTGTAGTTAAATGCAGGGGAGTTACCTCTCACAA11.8181818181818181No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG11.8181818181818181No Hit
ATCACTCACTGAGTGACATCAACATCATTGCGTCTCAAGGCACCAAACGA11.8181818181818181No Hit
ATGCTCCTTGTGTACCCAAGGATACATTCCGGCTGTTTATAATGATAGGC11.8181818181818181No Hit
GTGTTATCGTTCTTTAAGATTCCTCCAAAGTCTTCTTTCATAATCTTTTT11.8181818181818181No Hit
ATTGGGAGGTTTTATGTACTGATTTGCAATTAGCTCAAACTCCGCGACCT11.8181818181818181No Hit
ATCACCAATCCACTAATCAGGCATGAGAACAGAATGGTACTAGCCAGCAC11.8181818181818181No Hit
CAGCAACGTCCTGGTTGGATTCCCTCCACTAGTAGACCGTCATCTCTGAT11.8181818181818181No Hit
ACCTCGACCTGCTCATACACACACGTTGTCTAAATCATTTCCACTTCATC11.8181818181818181No Hit
CATTAACCTCTCGCCGGACATTTCCTCAACAGTCATTTCAGTTCAGTAGC11.8181818181818181No Hit
GCATAAAGAGTACTAGTCCCTGGCCCTGCTCTCATTAACTGGAAGAAATT11.8181818181818181No Hit
GCCTTTACCATGCAATCTTCTTGTGAGAACACCATTGCCACAATAATTGC11.8181818181818181No Hit
ATTCCAAGTCACAAATAAATAGGCAAGTCATAGTTGACAGTGATAACTGG11.8181818181818181No Hit
GAGAGAGGCAGCATGCGCAATGAGGGTATTAGAACTGACACCGGAGAAGG11.8181818181818181No Hit
GGAAGCCGGGGAGGAAGTCCCCCACATAGTGTCCTCCTTATAACAGCCCC11.8181818181818181No Hit
GCATTTTAACACAATTCGAGTCTCTGCAAATCTTCAGAGATTCACTTGGA11.8181818181818181No Hit
CAATTGGAAAATGTCCAAATTTTCCAAAAAGCACAACATTGCTCCTGGCC11.8181818181818181No Hit
CTTCAGTTTAGTACGGTATCCATAAGTTAGAAGTGTTTGCAGAGGCAATT11.8181818181818181No Hit
TGGAATGATAACACAGTTCGAGTCTGTGAAACTCTATAGAGATTCACTTG11.8181818181818181No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG11.8181818181818181No Hit
TCCCAAATATCCACAAAGTTCTGAGGCAATTCTTAGAGAATCTACAGCAC11.8181818181818181No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCATCCCATCAGGCCC11.8181818181818181No Hit
CTTCAGAAATGGCTCAATTCTGGCGTTCACTTCTTTCGACATTTGAGAAA11.8181818181818181No Hit
ATAGTGTCCTCATTATAAGGACCCCCAGGAAATCTCCCGTCCAGTATCAG11.8181818181818181No Hit
ATACAGCTCTGCTCAATGCATTTTGTGCAACCATGGCATACAACAAAATT11.8181818181818181No Hit
CAATGGATTCCAGCACTCTTGAACTGAGAAGCAGATATTGGGCTATAAGG11.8181818181818181No Hit
CGCATATAATGCAGAATTGCTAGTACTACTTGAAAATTAAAAAACACTCG11.8181818181818181No Hit
AGTTTGAACCATTTCAATCTTTAGTACCAAAGGCTGCCAGAAGCCAATAT11.8181818181818181No Hit
GACCCTCCGTGAGGGCTCAAGACGGGCGACTGCACCCTTACTGGACACTA11.8181818181818181No Hit
GTACAGTGAAATCTTCCCTGCGATAGACCGAACATTATGGATGCGTACTT11.8181818181818181No Hit
ATTATTGATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATGATCAA11.8181818181818181No Hit
CAATGGGATATTCTAGCGCAGCATAGAATACGAACATGGTGAGAATAATT11.8181818181818181No Hit
TTGAAAACTCATGTCTTGAAACGATGGAAGTTGTCCAGCAAACGAGAGTG11.8181818181818181No Hit
AGTGGACCCAAGCGCGTCTTTCAATTATACTTCATAAATTTATGGCCATC11.8181818181818181No Hit
GTACCAAAGGCTGCCAGAAGCCAATATAGTGGATTTGTGAGAACGCTATT11.8181818181818181No Hit
GTTTGAGACTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATAT11.8181818181818181No Hit
GAGCAACAGCAGGGCGACAAAAACATAATGGATTCCAACACTGTGTCAAG11.8181818181818181No Hit
CTCTTCACCTTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGTG11.8181818181818181No Hit
CTGTTGCAATCTGGACTTTACCTCTCTTAGCTTTATATATGAAAAGGATT11.8181818181818181No Hit
GCATACTCCGCACTCAGCAGGGGGCCTGACGCAGGACTCGATCCCAGGTC11.8181818181818181No Hit
TTAACATATAGGGTATTATTGGTTTCCGGAGTAGAATTTAGTGATTCATC11.8181818181818181No Hit
ATCCCAACACTGTGTCAAACTTTCAGGTAGACTGCTTTCTTTGGCATATC11.8181818181818181No Hit
TATTCCATGCCCTTGGCCGGACCATGGGCTGGCATTACCACAACATTGTT11.8181818181818181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)