FastQCFastQC Report
Fri 29 Apr 2022
000000000-K6NVC_l01_n01_FC_PCR_Neg_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K6NVC_l01_n01_FC_PCR_Neg_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG15.555555555555555No Hit
ATCCCAATGATACTTGCTGCAACAACGAGAGGATCACTTGAATCTCTGCA15.555555555555555No Hit
CATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGAGCAGCACTTCTGG15.555555555555555No Hit
CAGGATACACCATGGACACAGTCAACAGAACACATTATTCGAGAAATTCT15.555555555555555No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG15.555555555555555No Hit
ATCTGAACTCCTCTAGTGGATAGTTGTCCTCTTGGGATCACTCTTGTTCC15.555555555555555No Hit
CAATATCCCAATGATACTTGCTGCAACAACGAGAGGATCACTTGAATCTC15.555555555555555No Hit
GACTTCAGTCCTCATGTCAGATGTTCTGCCCTCGGTATTCCCCTTAAACG15.555555555555555No Hit
ATGCAGATAAAATCTGCATCGGCCACCAGTCAACAAACTCCACAGAAACT15.555555555555555No Hit
CCCTGGAGCTGCTGGTGCCTCCCTAAAGGGCGTCGCCACAATGGCAATGG15.555555555555555No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA15.555555555555555No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGATTTGTCCAAAATGCC15.555555555555555No Hit
GAACAGTGACCACAGAAGTGGCTCTTGGCCTAGTATGTGCCACTTGTGAA15.555555555555555No Hit
ATACCAGAAGCTGAAGAGGGAAATGACATTCCATGGAGCAAAGGAAGTTG15.555555555555555No Hit
GGTCTAAAAGATGATCTTATTGAAAATTTGCAGGCTTACCAGAAACGGAT15.555555555555555No Hit
CCATAGACCCCTTTTAATGAACGAGTTGGGTGTTCCATTTTATTTGGGAA15.555555555555555No Hit
GCAATAAAGGGAGTCGGGACAATGGTAATGGAACTAATTCGGATGATAAA15.555555555555555No Hit
GTTGTCATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)