FastQCFastQC Report
Mon 2 May 2022
000000000-K6NVC_l01_n01_FCC_2231_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K6NVC_l01_n01_FCC_2231_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397089
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC16640.4190496337093196No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG11430.2878447904625915No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT7980.2009625046274261No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA7900.19894784292690051No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT7820.19693318122637493No Hit
ATCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC6730.16948341555671398No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG6360.1601656051917832No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC6330.1594101070540861No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT6320.1591582743415204No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA6080.1531142892399437No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA6060.1526106238148123No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA5820.14656663871323558No Hit
TTTATATATAGCACGGGTCTATTGGAGCCCTTCCAATTGTCTCTGCAAAC5530.13926349004883037No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC5510.13875982462369896No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT5450.1372488283483048No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT5300.13347133765981933No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT5080.127931017983374No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG5080.127931017983374No Hit
ATATAAATGTGGCAGATTATAGTGTTGATTCTAGTTATGTGTGCTCAGGA4990.12566452357028274No Hit
GTACTAGCCAGCACTACGGCTAAGGCCATGGAGCAGATGGCTGGATCAAG4880.12289436373206007No Hit
ATATATAAATGTGGCAGATTATAGTGTTGATTCTAGTTATGTGTGCTCAG4810.12113153474410018No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC4740.11936870575614031No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC4700.11836137490587753No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT4680.11785770948074613No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC4610.11609488049278627No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT4470.11256922251686649No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA4450.1120655570917351No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA4430.1115618916666037No Hit
ATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTC4320.10879173182838105No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA4270.10753256826555256No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT4150.1045105757147642No Hit
ACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAACAGGTA4130.1040069102896328No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT4110.10350324486450141No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT4040.10174041587654153No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAACATCATGG3990.10048125231371305No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA3980.10022941960114734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)