FastQCFastQC Report
Mon 2 May 2022
000000000-K6NVC_l01_n01_FCC_2231_d08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K6NVC_l01_n01_FCC_2231_d08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555181
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC29670.5344203061704201No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG20880.37609356227968893No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT16490.2970202510532601No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA13620.24532539838359024No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC12840.23127592622946389No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT12340.2222698543357932No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT11820.21290353956637564No Hit
ATCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC11020.1984938245365025No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC10890.19615224584414812No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC10620.19128896702156592No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG10070.18138228793852815No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT9280.1671526943465284No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA9270.16697257290865503No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT8910.16048820114521212No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA8670.15616528663625018No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT8280.149140550559187No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT8080.1455381218017187No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA8010.14427727173660482No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG7880.14193569304425044No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG7700.13869350716252898No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA7690.13851338572465557No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA7600.13689229278379483No Hit
TTTATATATAGCACGGGTCTATTGGAGCCCTTCCAATTGTCTCTGCAAAC7410.13346998546419997No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT7400.13328986402632656No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA7290.131308528209719No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA7240.13040792102035192No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT6840.12320306350541534No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCATTCTGCTGT6750.12158197056455462No Hit
ATATATAAATGTGGCAGATTATAGTGTTGATTCTAGTTATGTGTGCTCAG6680.12032112049944073No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG6470.11653857030409903No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT6440.11599820599047879No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA6400.11527772023898512No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA6380.11491747736323829No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT6330.11401687017387123No Hit
ATACATGATAGGAGTCCCCATAGAACCCTTTTAATGAACGAGTTGGGTGT6320.1138367487359978No Hit
TTCTTAGACCATGAACCAATACTGTCGGTAAGCATCCCATCATAAATGAT6300.11347650586025099No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT6300.11347650586025099No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA6210.11185541291939025No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA6070.10933371278916246No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT6020.1084331055997954No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC6000.10807286272404855No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA5870.10573128403169416No Hit
GTATAACAATAGAGAGAATGGTTCTCTCCGCATTTGATGAAAGGAGGAAC5870.10573128403169416No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT5820.1048306768423271No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT5790.10429031252870685No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT5680.1023089767120993No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT5650.10176861239847906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)