FastQCFastQC Report
Mon 2 May 2022
000000000-K6NVC_l01_n01_FCC_1969_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K6NVC_l01_n01_FCC_1969_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences675290
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC46040.6817811606865198No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAG33470.495638910690222No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTT30170.44677101689644444No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT25310.37480193694560854No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA25090.37154407735935674No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT22740.3367442135971212No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA22340.3308208325312088No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19980.2958728842423255No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT18880.2795835863110664No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGCT18170.2690695849190718No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC17710.2622576966932725No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT17050.252484117934517No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA16960.25115135719468673No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA16580.24552414518206994No Hit
ACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTCACCAT16070.2379718343230316No Hit
GCATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAA15950.23619482000325787No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG15530.22997526988404982No Hit
CTATTGCTCCTTCATCTGTAAACGCTCTAAGTAGTATCAGAGCTTCAAGT14940.22123828281182897No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT14440.21383405647943848No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCT14180.2099838587865954No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA14090.20865109804676507No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG13800.20435664677397858No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA13600.20139495624102235No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA13310.19710050496823586No Hit
CTCTTGGTCTGGACATCAGAACTGCCACTCGTGAAGGAAAGCATATAGTG13120.19428689896192747No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC12950.19176946200891468No Hit
GCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATTCTTAACCTATGT12920.19132520842897127No Hit
GTAATGGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAA12910.19117712390232344No Hit
GGTTAATGCTCATTCCCAAGCAGAAAGTGACAGGGCCCCTTTGCATTAGA12630.18703075715618475No Hit
GTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATT12400.18362481304328512No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC12200.1806631225103289No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT11920.1765167557641902No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAA11490.17014912111833436No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC11370.1683721067985606No Hit
AAGTAGTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTT11290.16718743058537813No Hit
ACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCT11120.16466999363236534No Hit
CTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCT11080.1640776555257741No Hit
ACCTAACTGAAATGACTCTTGAGGAAATGTCAAGGGATTGGTTAATGCTC11070.1639295709991263No Hit
AGAATGGACCAGGCAGTAATGGGTAAAACCATCATATTGAAAGCAAACTT10900.16141213404611351No Hit
CCCTAAGAGGAAGAGGCAGCACTCTTGGTCTGGACATCAGAACTGCCACT10860.16081979593952228No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA10580.1566734291933836No Hit
GGGTAAAACCATCATATTGAAAGCAAACTTTAGTGTGATTTTTAATCGAC10510.15563683750684892No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC10330.15297131602718833No Hit
ATATAGTGGAGCGGATTCTGGAGGAAGAATCTGACGAGGCACTTAAAATG10120.1498615409675843No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT10090.14941728738764087No Hit
GAGCAAAAGCAGGTAGATGTTGAAAGATGAGTCTTCTAACCGAGGTCGAA9910.14675176590798028No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT9700.14364199084837626No Hit
GAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTA9620.14245731463519376No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA9450.139939877682181No Hit
GCATTAACCAATCCCTTGACATTTCCTCAAGAGTCATTTCAGTTAGGTAG9420.13949562410223756No Hit
TTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCATTAAC9390.1390513705222941No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG9370.1387552014689985No Hit
ACATAATGGATTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTT9200.13623776451598574No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG9180.1359415954626901No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT9130.13520117282945107No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG8800.1303143834500733No Hit
CTCCCATTCTCATCACTGCTTCTCCAAGTGAATCTCTGTAGAGTTTCAGA8780.13001821439677766No Hit
GAACTGGGTGATGCCCCATTCCTTGACCGGCTTCGCCGAGATCAGAAGTC8700.1288335381835952No Hit
ATACTACTTAGAGCGTTTACAGATGAAGGAGCAATAGTGGGCGAAATCTC8630.12779694649706053No Hit
CTTCAAACTTCTGGCTCAATTGTTCTCTCCACTTTCCGTTTCTGTTTTGG8510.1260199321772868No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG8460.12527950954404773No Hit
GTTTTACCCATTACTGCCTGGTCCATTCTAATGCAAAGGGGCCCTGTCAC8170.12098505827126123No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCACTTGGAGAA8150.12068888921796563No Hit
TTCCAGGACATACTGACGAGGATGTCAAAAATGCAATTGGGGTCCTCATC8040.11905995942483968No Hit
GTCCTGGAAGGGAAGGTAATGGTGAGATTTCGCCCACTATTGCTCCTTCA7940.1175791141583616No Hit
GAGCAATAGTGGGCGAAATCTCACCATTACCTTCCCTTCCAGGACATACT7930.11743102963171377No Hit
CTCTTGCTCCACTTCAAGCAATAGTTGTAAGGCTTGCATAAAGGTTATTT7790.11535784625864444No Hit
GTTTTGGAGGGAGTGGAGATCTCCCATTCTCATCACTGCTTCTCCAAGTG7750.11476550815205319No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG7660.1134327474122229No Hit
CTTAAAATGACTATCGCTTCAGTGCCTGCTTCACGCTACCTAACTGAAAT7500.11106339498585793No Hit
GTATCAGAGCTTCAAGTCGATTAAAAATCACACTAAAGTTTGCTTTCAAT7480.11076722593256232No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGATTCCAACACTGTGTCAAGC7470.11061914140591449No Hit
TTCCTTGACCGGCTTCGCCGAGATCAGAAGTCCCTAAGAGGAAGAGGCAG7410.10973063424602764No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTC7380.1092863806660842No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC7270.10765745087295828No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT7250.10736128181966266No Hit
CTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTCCTC7040.10425150676005865No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG7010.1038072531801152No Hit
TTTATATATAGCACGGGTCTATTGGAGCCCTTCCAATTGTCTCTGCAAAC6940.10277066149358054No Hit
GTCCATTCTAATGCAAAGGGGCCCTGTCACTTTCTGCTTGGGAATGAGCA6940.10277066149358054No Hit
ATATATAAATGTGGCAGATTATAGTGTTGATTCTAGTTATGTGTGCTCAG6910.10232640791363712No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC6890.10203023886034149No Hit
CTTCAGTGCCTGCTTCACGCTACCTAACTGAAATGACTCTTGAGGAAATG6840.10128981622710242No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)