FastQCFastQC Report
Fri 25 Mar 2022
000000000-K6NDM_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K6NDM_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17960411
Sequences flagged as poor quality0
Sequence length8
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTAC10850396.041281572008569No Hit
CGAGGCTG10430035.807233475893174No Hit
CGTACTAG6605333.677716506598874No Hit
CCGAGTGT5422653.0192237805693867No Hit
CAGAGAGG5173322.88040179035992No Hit
AAGAGGCA2203771.2270153505952621No Hit
AGGCAGAA1768130.9844596540691636No Hit
AAGATGCA1602350.8921566438540856No Hit
AAGAAGCA1228030.6837427049971183No Hit
CTCTATAC778830.43363706988665235No Hit
CTCTCTCC750770.41801381939422205No Hit
AAGCAGAA679900.37855481146840125Illumina Single End Adapter 1 (100% over 8bp)
AAACAACA606220.337531251372811No Hit
AAGCAGCA589400.3281662095594583No Hit
CGAGTCTG540630.30101204254178815No Hit
CTCTTTAC518880.28890207467969414No Hit
AAACAAAA487800.2715973481898605No Hit
AAAAAAAA468480.2608403560475314No Hit
AAAAAACA461520.25696516633166133No Hit
AATATGCA438580.24419263011297457No Hit
CGAGTGTC413560.23026199122057953No Hit
CTCTCTAA406190.2261585216507573No Hit
AAATAAAA384510.21408752839787462No Hit
AAAAAGCA376720.20975021117278442No Hit
AAGCTGCA368080.20493963083584224No Hit
AATCAGCA364530.20296306136869585No Hit
AATAAGCA362980.20210005216473054No Hit
AAATAACA362970.20209448436341462No Hit
CAACAACA359530.2001791607107432No Hit
CGAGACTG355150.19774046373437665TruSeq Adapter, Index 15 (100% over 8bp)
AAACAGAA348360.19395992664087697No Hit
AAACAGCA346870.19313032424480708No Hit
AATCAGAA318740.1774680991431655No Hit
AATCAACA318370.177262090494477No Hit
AAAATGCA297990.16591491141266201No Hit
AAACTACA291040.16204528949810781No Hit
CATCTACA275880.15360450270319537No Hit
CAAAAAAA273830.15246310343343478No Hit
AAAATACA272030.15146089919657182No Hit
CAATAAAA271280.15104331409787894No Hit
AATCTGCA265540.1478473961425493No Hit
CATCAACA264900.14749105685833136No Hit
CAATAACA262490.14614921674119818No Hit
CAACAAAA257440.143337477076666No Hit
CTCTATAA252500.14058698322660879No Hit
AATAAACA244630.13620512359099132No Hit
CAGAGAGT244190.13596014033309148No Hit
GTAGAGGA243950.13582651310150975No Hit
CAAAAACA243270.1354479026120282No Hit
AATCAAAA240590.13395573185936557No Hit
CAGATAGG233530.13002486413033643No Hit
CAACTACA233340.12991907590533422No Hit
AAAATAAA222580.12392812168942015No Hit
AATCTACA212790.11847724420114886No Hit
AATAAAAA212320.1182155575393013No Hit
CTCTTTCC208990.11636147970110484Illumina Single End PCR Primer 1 (100% over 8bp)
AATATACA208390.11602741162215052No Hit
CAGATGCA199350.11099411923257213No Hit
AAACAATA197020.10969682152596619No Hit
CATATACA192750.10731937036407461No Hit
AAATAATA188110.10473591055349457No Hit
AAAGAAAA187840.10458557991796513No Hit
AAGAAACA187830.10458001211664922No Hit
AAAAAGAA185960.10353883327057493No Hit
CGTACTAA183080.10193530649159421No Hit
AAATTACA182710.10172929784290571No Hit
CTCTATCC182620.10167918763106255No Hit
CATCTATA182040.10135625515474006No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)