FastQCFastQC Report
Fri 22 Apr 2022
000000000-K5J99_l01_n01_hTF_PGC03lib.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K5J99_l01_n01_hTF_PGC03lib.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5486791
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT152290.27755750127898077No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT135210.24642819454941878No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTC125700.2290956590108863No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG104250.1900017697047327No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCT103130.18796050368967945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)