FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix38_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix38_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107284
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC14621.3627381529398606No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA10771.0038775586294322No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC8160.7605980388501546No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA7570.7056038179038813No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA6600.6151895902464487No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCC5750.5359606278662242No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG5440.5070653592334365No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAA5010.46698482532344054No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA4650.4334290294918161No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT4220.3933484955818202No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4060.3784348085455427No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT4010.3737742813467059No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3710.34581111815368554No Hit
GTCTAAGGATATCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC3560.33182953655717534No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG3500.32623690391857124No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT3370.31411953320159575No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC3320.30945900600275905No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAG3290.306662689683457No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG3130.29174900264717946No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGC3120.2908168972074121No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAAAAGAACC3070.28615637000857536No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG2980.2777674210506692No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC2900.27031057753253046No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA2870.2675142612132284No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT2820.2628537340143917No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC2690.2507363632974162No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA2620.24421162521904477No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT2600.2423474143395101No Hit
GTGTCCTTACAAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA2570.23955109802020805No Hit
CTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTG2520.2348905708213713No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATAT2440.22743372730323255No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAGGTT2400.22370530554416315No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC2360.21997688378509378No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA2310.21531635658625703No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATATGGTACCTTCG2310.21531635658625703No Hit
ATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2290.21345214570672233No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT2270.21158793482718763No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAAAAGAACCCC2240.20879161850788563No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA2240.20879161850788563No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC2240.20879161850788563No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT2230.20785951306811828No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCCCTCCCGCTTACTATCAACTG2230.20785951306811828No Hit
ATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA2140.19947056411021213No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC2110.1966742477909101No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA2110.1966742477909101No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC2090.1948100369113754No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG2080.19387793147160806No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT2070.1929458260318407No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG2060.19201372059207336No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC2040.19014950971253866No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA1960.18269266619439992No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA1920.17896424443533052No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC1910.1780321389955632No Hit
CTCTTATCCCTCTCAGCGACATCTCCATGCTTGGGGTCATGTCGGGCAGT1890.1761679281160285No Hit
TGCTGGAACAGTGTCCTTACAAATCCACTGTACCGGCTTCTGGTTGCCTT1860.17337161179672644No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA1840.17150740091719177No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAA1840.17150740091719177No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA1810.16871108459788972No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC1790.16684687371835502No Hit
ACTTCATTCTGAGTGCGGGGTTCTTTTCTTGCCTTCCTGATGTGTACTTT1780.16591476827858767No Hit
CAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG1780.16591476827858767No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC1750.16311845195928562No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT1750.16311845195928562No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT1730.16125424107975095No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA1730.16125424107975095No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1730.16125424107975095No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA1710.15939003020021625No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA1700.1584579247604489No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA1680.1565937138809142No Hit
ACATTAGATCTCTCAGTTCTTTTATTCTCTCCATATTGAATATAATTGAC1670.15566160844114688No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTCTTGACTGATGATCC1670.15566160844114688No Hit
CATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAATGAAGTGGATGATG1650.15379739756161218No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCAC1630.15193318668207748No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG1630.15193318668207748No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT1620.15100108124231013No Hit
GATTGGAGGAGTAAGGATGGTGGATATCCTTAGACAGAATCCAACTGAGG1610.15006897580254278No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG1600.14913687036277545No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG1600.14913687036277545No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC1600.14913687036277545No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA1590.1482047649230081No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG1590.1482047649230081No Hit
ATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGC1580.14727265948324075No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGATGTAC1580.14727265948324075No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC1580.14727265948324075No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT1570.1463405540434734No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT1560.14540844860370605No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC1560.14540844860370605No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT1540.14354423772417135No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC1530.142612132284404No Hit
GAAAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC1520.14168002684463665No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT1520.14168002684463665No Hit
GTTCTTTTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT1510.1407479214048693No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAG1500.13981581596510195No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA1480.13795160508556728No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT1480.13795160508556728No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATATCCA1470.13701949964579993No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT1460.13608739420603258No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG1460.13608739420603258No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT1450.13515528876626523No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT1440.13422318332649788No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA1390.12956265612766116No Hit
ACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA1370.12769844524812646No Hit
CAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGT1370.12769844524812646No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAAT1370.12769844524812646No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCATCCGAATTCTTTTATTC1330.12397002348905709No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT1320.12303791804928974No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA1300.12117370716975505No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGGCGGCC1300.12117370716975505No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGAT1300.12117370716975505No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC1290.1202416017299877No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA1290.1202416017299877No Hit
TCTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCA1290.1202416017299877No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAGTAGGATG1290.1202416017299877No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC1280.11930949629022035No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG1280.11930949629022035No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA1250.1165131799709183No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT1250.1165131799709183No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT1240.11558107453115098No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA1240.11558107453115098No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTC1240.11558107453115098No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG1230.11464896909138363No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT1230.11464896909138363No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT1230.11464896909138363No Hit
CTCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT1220.11371686365161628No Hit
GAACTGAGAGGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC1210.11278475821184893No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA1200.11185265277208158No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT1200.11185265277208158No Hit
CTCCCAACCATTGTGAATTCTTCATACCCTTCATGTACTCTTATTTTCAG1200.11185265277208158No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA1190.11092054733231423No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTCTT1190.11092054733231423No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT1190.11092054733231423No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAAAA1190.11092054733231423No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC1180.10998844189254689No Hit
GTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTG1170.10905633645277954No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA1170.10905633645277954No Hit
GTCCTTACAAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA1170.10905633645277954No Hit
CAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCC1170.10905633645277954No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT1160.10812423101301219No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT1160.10812423101301219No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG1150.10719212557324484No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC1150.10719212557324484No Hit
GTACACATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAATGAAGTGGA1150.10719212557324484No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA1140.10626002013347749No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT1140.10626002013347749No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT1120.10439580925394282No Hit
CTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTG1120.10439580925394282No Hit
GGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCA1120.10439580925394282No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG1120.10439580925394282No Hit
CTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCA1120.10439580925394282No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT1110.10346370381417547No Hit
ACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGG1090.10159949293464077No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCATCC1090.10159949293464077No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA1090.10159949293464077No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC1090.10159949293464077No Hit
GTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTG1080.10066738749487342No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT1080.10066738749487342No Hit
GTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGT1080.10066738749487342No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)