FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix37_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix37_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71464
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC5720.8004030001119445No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC5200.7276390910108587No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC4370.6114966976379715No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA3880.5429307063696406No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3760.5261390350386208No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA3490.48835777454382623No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA3460.4841598567110713No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA3070.42958692488525696No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT2920.40859733572148216No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT2760.3862084406134557No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC2680.37501399305944255No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2630.36801746333818425No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA2570.35962162767267436No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG2320.32463897906638306No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA2320.32463897906638306No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT2240.31344453151236984No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2230.3120452255681182No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA2190.30644800179111165No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG1940.2714653531848203No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC1800.2518750699652972No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC1770.24767715213254224No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT1770.24767715213254224No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT1740.2434792342997873No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT1720.240680622411284No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCAC1710.23928131646703235No Hit
ATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAG1650.23088548080152246No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG1650.23088548080152246No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA1640.22948617485727077No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG1580.22109033919176088No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA1550.21689242135900594No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1510.21129519758199936No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA1470.20569797380499272No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT1450.2028993619164894No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA1400.1959028321952312No Hit
CAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGC1400.1959028321952312No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA1370.19170491436247622No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG1340.18750699652972128No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT1310.1833090786969663No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC1280.17911116086421133No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG1280.17911116086421133No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT1280.17911116086421133No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC1270.17771185491995972No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC1250.1749132430314564No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG1240.17351393708720475No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA1210.16931601925444978No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC1200.16791671331019814No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG1200.16791671331019814No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA1190.1665174073659465No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA1190.1665174073659465No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1170.1637187954774432No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC1160.16231948953319153No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG1150.1609201835889399No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT1140.15952087764468822No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA1130.1581215717004366No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT1120.15672226575618492No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA1120.15672226575618492No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT1110.15532295981193328No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA1110.15532295981193328No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA1110.15532295981193328No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT1110.15532295981193328No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA1090.15252434792342998No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT1090.15252434792342998No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT1080.15112504197917834No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG1080.15112504197917834No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA1080.15112504197917834No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG1070.14972573603492667No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT1070.14972573603492667No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC1060.14832643009067503No Hit
GTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA1050.14692712414642337No Hit
GCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTCCT1050.14692712414642337No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA1040.14552781820217173No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG1020.14272920631366842No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC1010.14132990036941676No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC1010.14132990036941676No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA1000.13993059442516512No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAG1000.13993059442516512No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC980.1371319825366618No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT980.1371319825366618No Hit
GCCTTGATCATGCAATCCTCTTGTGAGAATACCATGGCCACAATTATTGC970.13573267659241015No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT970.13573267659241015No Hit
GGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCA970.13573267659241015No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAGTAGGATG960.1343333706481585No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT960.1343333706481585No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCA950.13293406470390687No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT950.13293406470390687No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC940.1315347587596552No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT940.1315347587596552No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA930.13013545281540356No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA930.13013545281540356No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT920.12873614687115192No Hit
GAACTGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC920.12873614687115192No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTAC910.12733684092690026No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA910.12733684092690026No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT900.1259375349826486No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT900.1259375349826486No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC900.1259375349826486No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC890.12453822903839697No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA880.12313892309414531No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC880.12313892309414531No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG880.12313892309414531No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG880.12313892309414531No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA880.12313892309414531No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA880.12313892309414531No Hit
GGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCCCCTAAC870.12173961714989365No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT870.12173961714989365No Hit
CTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTG870.12173961714989365No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC870.12173961714989365No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG870.12173961714989365No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA860.120340311205642No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT850.11894100526139036No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA850.11894100526139036No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG850.11894100526139036No Hit
GATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGC850.11894100526139036No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT850.11894100526139036No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA840.1175416993171387No Hit
ATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTG840.1175416993171387No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT840.1175416993171387No Hit
ACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAG840.1175416993171387No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG830.11614239337288705No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTC830.11614239337288705No Hit
GTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTG820.11474308742863538No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC820.11474308742863538No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC810.11334378148438376No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC810.11334378148438376No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC810.11334378148438376No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG810.11334378148438376No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC810.11334378148438376No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAAGAGGATT800.1119444755401321No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC800.1119444755401321No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC800.1119444755401321No Hit
AAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGA790.11054516959588044No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC790.11054516959588044No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT790.11054516959588044No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA790.11054516959588044No Hit
GTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAA780.10914586365162879No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT780.10914586365162879No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG770.10774655770737715No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC770.10774655770737715No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTT770.10774655770737715No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC760.10634725176312548No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC760.10634725176312548No Hit
GATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATT760.10634725176312548No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT750.10494794581887383No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC750.10494794581887383No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG750.10494794581887383No Hit
ACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGG750.10494794581887383No Hit
GTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGT750.10494794581887383No Hit
ATTGATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATC750.10494794581887383No Hit
CCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAG740.10354863987462219No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC740.10354863987462219No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA740.10354863987462219No Hit
CCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAG740.10354863987462219No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA720.10075002798611889No Hit
CAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTTC720.10075002798611889No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT720.10075002798611889No Hit
CCTCTTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGC720.10075002798611889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)