FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix36_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix36_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146390
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC10590.7234100689937837No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC9580.654416285265387No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA8330.5690279390668762No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC7950.5430698818225289No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG7450.5089145433431246No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC7260.4959355147209509No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT6780.46314638978072276No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT6700.45768153562401803No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC5790.39551881959150215No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC5700.3893708586652094No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA5360.36614522849921444No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG5190.35453241341621694No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC5120.3497506660291003No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG5000.34155338479404335No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA4840.3306236764806339No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA4550.31081358016257943No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG4500.30739804631463896No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT4460.3046656192362866No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA4280.29236969738370105No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC4220.28827105676617254No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA4180.28553862968782023No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4170.28485552291823213No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA3790.25889746567388483No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC3390.23157319489036138No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG3140.2144955256506592No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG3100.21176309857230685No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC3080.21039688503313064No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT2990.2042489241068379No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA2960.20219960379807364No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA2890.19741785641095702No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT2860.1953685361021928No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC2800.19126989548466425No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT2760.18853746840631191No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2750.1878543616367238No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2750.1878543616367238No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC2720.18580504132795955No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2700.18443882778878337No Hit
AGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTG2700.18443882778878337No Hit
AAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCA2690.1837557210191953No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2670.18238950748001914No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA2660.18170640071043104No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG2640.18034018717125488No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT2620.1789739736320787No Hit
ATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTC2610.1782908668624906No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2600.17760776009290252No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA2580.17624154655372634No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT2560.17487533301455016No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT2560.17487533301455016No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC2520.17214290593619783No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT2480.1694104788578455No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA2450.16736115854908123No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT2430.16599494500990505No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA2410.16462873147072887No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT2360.16121319762278846No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG2350.16053009085320036No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC2330.15916387731402418No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2330.15916387731402418No Hit
TTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAG2310.15779766377484802No Hit
GAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTT2300.15711455700525992No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG2280.15574834346608374No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2280.15574834346608374No Hit
GTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATAC2270.15506523669649566No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA2260.15438212992690759No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT2190.14960038253979097No Hit
CCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATA2170.1482341690006148No Hit
CTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATACAG2160.1475510622310267No Hit
CCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTC2120.14481863515267437No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA2100.1434524216134982No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2070.14140310130473394No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG2040.13935378099596968No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC2040.13935378099596968No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA2010.1373044606872054No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG1990.13593824714802924No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA1990.13593824714802924No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA1980.13525514037844116No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA1950.1332058200696769No Hit
GTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGC1940.1325227133000888No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC1910.13047339299132454No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT1900.12979028622173647No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA1890.12910717945214836No Hit
GTGCTATGGGGCATTCACCATCCATCTACTAGTGCTGACCAACAAAGTCT1880.1284240726825603No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA1860.12705785914338413No Hit
ACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCAT1840.12569164560420792No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC1830.12500853883461985No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAAC1820.12432543206503176No Hit
GGTGTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGA1800.12295921852585559No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT1790.1222761117562675No Hit
ATTTTGGACATTTTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGG1770.12090989821709132No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC1750.11954368467791517No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA1750.11954368467791517No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG1740.11886057790832708No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC1740.11886057790832708No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT1730.11817747113873897No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1710.11681125759956282No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC1700.11612815082997473No Hit
TTCACCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCA1700.11612815082997473No Hit
GGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCATCCATCTACTAGTGC1690.11544504406038664No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG1690.11544504406038664No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA1690.11544504406038664No Hit
GCCCCAAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGT1680.11476193729079855No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT1680.11476193729079855No Hit
TGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCAAATGA1670.11407883052121047No Hit
CTCTTAGCTCCTCATAATCGATGAAATCTCCTGGGTAACACGTTCCATTG1660.11339572375162238No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC1640.1120295102124462No Hit
CCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATG1630.11134640344285812No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG1610.10998018990368194No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA1590.10861397636450576No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC1590.10861397636450576No Hit
TCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT1570.1072477628253296No Hit
GCTATGGGGCATTCACCATCCATCTACTAGTGCTGACCAACAAAGTCTCT1570.1072477628253296No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1560.10656465605574152No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC1550.10588154928615343No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA1550.10588154928615343No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC1550.10588154928615343No Hit
ACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTCCTC1540.10519844251656532No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC1530.10451533574697726No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG1510.10314912220780108No Hit
CTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATACAGCA1510.10314912220780108No Hit
CCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAA1510.10314912220780108No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA1500.10246601543821299No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC1500.10246601543821299No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT1480.10109980189903682No Hit
CTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTG1470.10041669512944873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)