FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix34_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix34_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174509
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC10670.611429782991135No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC9810.5621486570893192No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG9640.5524070391784951No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC8970.5140136038828943No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC7560.43321547885782397No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT7340.4206086792085222No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT6970.3994063343437875No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC6590.3776309531313571No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA6310.3615859353958822No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA6100.34955217209427597No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC5750.3294958999249322No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG5540.317462136623326No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA5350.30657444601711087No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG5290.30313622793093764No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA5240.30027104619246No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG5170.29625979175859124No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA5100.2922485373247225No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT5050.28938335558624484No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA4690.2687540470692056No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT4620.26474279263533684No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA4540.2601585018537726No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC4520.2590124291583815No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4360.24984384759525297No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC4260.24411348411829764No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG3770.21603470308121644No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT3720.21316952134273875No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC3420.19597843091187275No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA3390.19425932186878614No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG3390.19425932186878614No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA3340.19139414013030848No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG3230.1850907403056576No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC3120.17878734048100672No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA3090.1770682314379201No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT3080.17649519509022457No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA3050.17477608604713799No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3050.17477608604713799No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA2930.16789964987479156No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG2910.16675357717940048No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA2810.16102321370244516No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT2750.15758499561627193No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC2740.1570119592685764No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2690.15414677753009873No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC2660.15242766848701214No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA2630.15070855944392553No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT2610.14956248674853445No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2600.14898945040083894No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC2590.1484164140531434No Hit
AGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTG2570.14727034135775233No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2540.1455512323146657No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA2540.1455512323146657No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT2510.1438321232715791No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC2490.14268605057618805No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC2490.14268605057618805No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC2470.14153997788079697No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2430.13924783249001485No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT2420.1386747961423193No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG2410.13810175979462377No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2400.13752872344692824No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG2400.13752872344692824No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT2400.13752872344692824No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2360.1352365780561461No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC2350.13466354170845057No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG2340.13409050536075504No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT2330.1335174690130595No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG2330.1335174690130595No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC2320.13294443266536396No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA2320.13294443266536396No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT2300.13179835996997288No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT2300.13179835996997288No Hit
GAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTT2290.13122532362227735No Hit
AAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCA2290.13122532362227735No Hit
GTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATAC2270.1300792509268863No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG2200.12606799649301756No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA2200.12606799649301756No Hit
ATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTC2190.12549496014532202No Hit
CCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATA2170.12434888744993094No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT2170.12434888744993094No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT2160.12377585110223542No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC2130.12205674205914882No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2090.11976459666836667No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC2070.11861852397297559No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG2070.11861852397297559No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT2060.11804548762528007No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT2060.11804548762528007No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC2050.11747245127758454No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT2050.11747245127758454No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC2030.11632637858219347No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA2030.11632637858219347No Hit
CTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTG2020.11575334223449794No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC2020.11575334223449794No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA1990.11403423319141133No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1980.11346119684371579No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA1980.11346119684371579No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1970.11288816049602025No Hit
CCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTC1970.11288816049602025No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA1960.11231512414832472No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA1940.11116905145293367No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG1930.11059601510523813No Hit
TGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCAAATGA1900.10887690606215153No Hit
TTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAG1900.10887690606215153No Hit
TCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT1890.10830386971445599No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA1860.10658476067136938No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC1860.10658476067136938No Hit
CTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATACAG1850.10601172432367384No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA1840.1054386879759783No Hit
ACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCAT1830.10486565162828279No Hit
GCCCCAAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGT1830.10486565162828279No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA1800.10314654258519618No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC1800.10314654258519618No Hit
GCATGAGGACATGCTGCCGTTACACCTTTGTTCGAGTCATGATTGGGCCA1790.10257350623750065No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA1780.10200046988980511No Hit
CCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCC1780.10200046988980511No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA1780.10200046988980511No Hit
GTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTACATTGTGGAAACAT1780.10200046988980511No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC1770.10142743354210958No Hit
GGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATG1760.10085439719441404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)