FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix33_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix33_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204725
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA10040.490413969959702No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG9790.4782024667236537No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC9240.4513371596043473No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC6410.3131029429722799No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC6360.31066064232507024No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC6290.3072414214189767No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA6140.2999145194773477No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC6130.2994260593479057No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA6060.2960068384418122No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT5530.27011845158138964No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA5330.26034924899255096No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA4930.2408108438148736No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG4920.24032238368543166No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA4780.2334839418732446No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC4760.2325070216143607No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA4620.22566857980217364No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC4490.2193185981194285No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA4460.2178532177311027No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA4310.21052631578947367No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG4200.2051532543656124No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA4070.19880327268286727No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA4050.1978263524239834No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG4040.19733789229454146No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT4030.19684943216509954No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG3920.19147637074123824No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT3910.19098791061179632No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT3900.19049945048235437No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC3780.18463792892905118No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG3750.18317254854072537No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG3730.1821956282818415No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA3660.17877640737574796No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG3640.17779948711686408No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA3570.17438026621077055No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3510.17144950543411894No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3500.170961045304677No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC3470.1694956649163512No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC3450.16851874465746733No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3440.1680302845280254No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA3420.16705336426914152No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG3410.1665649041396996No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT3390.16558798388081575No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC3260.15923800219807058No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC3250.15874954206862865No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA3220.15728416168030285No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA3220.15728416168030285No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG3200.156307241421419No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT3160.15435340090365124No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG3160.15435340090365124No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC3120.1523995603858835No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT3110.15191110025644158No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT3070.14995725973867385No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT3060.1494687996092319No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA3050.14898033947978997No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA3050.14898033947978997No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3010.14702649896202225No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC2980.14556111857369644No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2980.14556111857369644No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT2980.14556111857369644No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA2970.1450726584442545No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG2930.14311881792648676No Hit
GGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAG2920.14263035779704483No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA2910.14214189766760288No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA2910.14214189766760288No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC2910.14214189766760288No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC2890.141164977408719No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA2880.14067651727927707No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2850.13921113689095127No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2850.13921113689095127No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC2780.13579191598485774No Hit
GGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTC2780.13579191598485774No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC2770.13530345585541578No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG2750.13432653559653193No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA2710.13237269507876417No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG2710.13237269507876417No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT2700.13188423494932225No Hit
GACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG2660.12993039443155452No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT2610.12748809378434484No Hit
GTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT2610.12748809378434484No Hit
AAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT2600.1269996336549029No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2530.12358041274880938No Hit
GTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCC2480.12113811210159971No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG2460.12016119184271583No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC2440.11918427158383196No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA2410.11771889119550616No Hit
GTGTCACTGTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGAT2400.11723043106606423No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA2390.1167419709366223No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC2370.11576505067773843No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT2340.11429967028941262No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2340.11429967028941262No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG2320.11332275003052876No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT2310.11283428990108682No Hit
ACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCTAC2280.11136890951276102No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT2250.10990352912443521No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA2240.10941506899499329No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG2240.10941506899499329No Hit
TGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGA2210.10794968860666748No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2210.10794968860666748No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG2200.10746122847722554No Hit
ATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGACA2200.10746122847722554No Hit
GCCCCATTGTCTGGGCCAGAAATTCCAATTGTTAGCCAATTGATGCCATC2190.1069727683477836No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2190.1069727683477836No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG2180.10648430821834168No Hit
GTATGGAGAGGGCACTTCACCAATAGGACAGCTCATTAGGGTTCGATATG2160.10550738795945781No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC2150.10501892783001587No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC2140.10453046770057393No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2120.10355354744169007No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA2120.10355354744169007No Hit
GTCTGAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATG2100.1025766271828062No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC2100.1025766271828062No Hit
ACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA2080.10159970692392234No Hit
CATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCCAACTCTTGATAGT2060.10062278666503846No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATTCCGTTGTAC2050.10013432653559654No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA2050.10013432653559654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)