FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix31_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix31_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177746
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC8310.4675210693911537No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG7610.428139029851586No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC7570.4258886275921821No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC6140.3454367468184938No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC6100.34318634455909No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT5590.3144937157516906No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC5530.3111181123625848No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT5530.3111181123625848No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA4960.2790498801660797No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA4960.2790498801660797No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA4850.27286127395271903No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT4770.26836046943391134No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA4740.26667266773935844No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT4730.26611006717450747No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA4610.2593588603962958No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG4390.24698164796957456No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC4230.2379800389319591No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG4200.23629223723740622No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC4100.23066623158889654No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3980.2239150248106849No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC3890.2188516197270262No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3850.21660121746762234No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC3650.205349206170603No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC3550.19972320052209333No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC3540.19916059995724236No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG3510.19747279826268946No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA3420.19240939317903075No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3310.1862207869656701No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC3250.18284518357656432No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT3160.1777817784929056No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA2990.16821756889043915No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG2960.16652976719588627No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA2940.16540456606618434No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT2920.16427936493648237No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA2880.16202896267707853No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC2870.16146636211222756No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT2860.1609037615473766No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT2860.1609037615473766No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA2840.15977856041767466No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2770.15584035646371788No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC2750.15471515533401595No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT2720.15302735363946304No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2640.14852654912065533No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC2620.1474013479909534No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC2590.1457135462964005No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG2590.1457135462964005No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT2550.14346314403699661No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2510.14121274177759274No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG2460.1383997389533379No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC2400.1350241355642321No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2380.13389893443453016No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG2370.13333633386967922No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT2360.13277373330482822No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG2360.13277373330482822No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA2350.13221113273997726No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA2350.13221113273997726No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA2310.12996073048057338No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG2300.12939812991572244No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT2300.12939812991572244No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG2280.1282729287860205No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC2270.12771032822116954No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG2270.12771032822116954No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG2260.12714772765631854No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2250.1265851270914676No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA2250.1265851270914676No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2250.1265851270914676No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA2240.12602252652661664No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC2220.1248973253969147No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG2220.1248973253969147No Hit
GACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTC2190.12320952370236181No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA2170.12208432257265986No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT2160.1215217220078089No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA2160.1215217220078089No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC2150.12095912144295792No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG2150.12095912144295792No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT2150.12095912144295792No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC2150.12095912144295792No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC2140.12039652087810697No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2130.11983392031325599No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT2110.11870871918355407No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG2110.11870871918355407No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA2030.11420791466474634No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT2030.11420791466474634No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG2020.11364531409989535No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA1990.11195751240534245No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA1980.1113949118404915No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT1980.1113949118404915No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT1950.10970711014593859No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG1940.10914450958108762No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA1920.10801930845138567No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA1920.10801930845138567No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC1910.10745670788653472No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA1900.10689410732168375No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT1900.10689410732168375No Hit
CTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTG1900.10689410732168375No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA1880.10576890619198182No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC1870.10520630562713086No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG1870.10520630562713086No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC1870.10520630562713086No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC1850.10408110449742893No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA1850.10408110449742893No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT1820.10239330280287602No Hit
AAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCA1820.10239330280287602No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC1810.10183070223802504No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC1810.10183070223802504No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC1790.1007055011083231No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC1780.10014290054347215No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT1780.10014290054347215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)