FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix20_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix20_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110016
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT5190.47174956369982546No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG5110.464477894124491No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT4810.4372091332169866No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA4320.3926701570680628No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4310.391761198371146No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC4150.377217859220477No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC3780.343586387434555No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT3570.3244982547993019No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG3500.3181355439208842No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3470.31540866783013377No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT3380.3072280395578825No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG3300.299956369982548No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3270.29722949389179754No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT3090.28086823734729494No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC3050.27723240255962767No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG3040.2763234438627109No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT2950.26814281559045955No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC2790.2535994764397906No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT2710.24632780686445607No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA2500.22723967422920302No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC2390.2172411285631181No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC2350.21360529377545084No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA2280.20724258289703318No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2230.20269778941244912No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2120.19269924374636416No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2080.18906340895869692No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT2020.18360965677719604No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA2020.18360965677719604No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC2010.18270069808027922No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA2000.1817917393833624No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG1980.1799738219895288No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC1960.17815590459569516No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA1920.17452006980802792No Hit
GACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTC1850.16815735892961023No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG1820.1654304828388598No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC1810.164521524141943No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT1790.16270360674810938No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA1780.16179464805119254No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA1770.16088568935427575No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA1770.16088568935427575No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT1740.15815881326352532No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT1730.15724985456660848No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT1710.15543193717277487No Hit
CTGCATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA1700.15452297847585805No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC1690.15361401977894126No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA1680.15270506108202442No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC1630.14816026759744036No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC1620.14725130890052357No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG1590.14452443280977312No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA1570.1427065154159395No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA1550.14088859802210588No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG1550.14088859802210588No Hit
CTCTTTGGGTCGCCCTCTGATTAGTTCAACCCAGAAGCAAGGTCTTATAC1540.13997963932518906No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG1540.13997963932518906No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC1490.135434845840605No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA1480.13452588714368818No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC1460.13270796974985455No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA1450.13179901105293776No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA1430.12998109365910412No Hit
CCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTC1400.1272542175683537No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG1390.12634525887143688No Hit
ACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTT1390.12634525887143688No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCTGGCCAAGACCA1380.12543630017452007No Hit
GAATTTACTTTGTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAG1380.12543630017452007No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAT1360.12361838278068643No Hit
GCATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG1350.12270942408376963No Hit
GTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCAAT1340.12180046538685282No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA1320.1199825479930192No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT1310.11907358929610237No Hit
TTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTG1310.11907358929610237No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT1300.11816463059918557No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1290.11725567190226877No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATATCCTG1290.11725567190226877No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG1290.11725567190226877No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA1270.11543775450843514No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC1270.11543775450843514No Hit
ATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTC1270.11543775450843514No Hit
CTTTGGGTCTTATTGCTATTTCCGGCTTGAACTTCTTGCTGTATCTTGAT1260.11452879581151831No Hit
GTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT1260.11452879581151831No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG1250.11361983711460151No Hit
CTCTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAAT1250.11361983711460151No Hit
CGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCC1250.11361983711460151No Hit
CCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACACCATTGCC1240.1127108784176847No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTGCATATGTATCCTAT1230.11180191972076789No Hit
GCATGAGGACATGCTGCCGTTACACCTTTGTTCGAGTCATGATTGGGCCA1220.11089296102385109No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA1210.10998400232693425No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA1210.10998400232693425No Hit
CTCTTAGCTCCTCATAATCGATGAAATCTCCTGGGTAACACGTTCCATTG1200.10907504363001745No Hit
TGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCAAATGA1200.10907504363001745No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA1190.10816608493310065No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG1170.10634816753926701No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC1170.10634816753926701No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTGCATATGTA1160.1054392088423502No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT1160.1054392088423502No Hit
CTTCTTTCCCTTTATCATTAATGTAGGATTTGCTGAGCTTTGGGTATGAA1160.1054392088423502No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG1150.10453025014543339No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA1150.10453025014543339No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC1150.10453025014543339No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA1150.10453025014543339No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT1140.10362129144851659No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC1140.10362129144851659No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG1120.10180337405468295No Hit
TCTGTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGA1120.10180337405468295No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG1120.10180337405468295No Hit
CGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAAGAT1110.10089441535776614No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1110.10089441535776614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)