FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix14_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix14_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195894
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC9880.5043543957446374No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC8400.42880333241446905No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC8210.41910420941937987No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT7670.39153828090702114No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA6950.3547837095572095No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC6790.34661602703502914No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG6720.34304266593157523No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC6080.3103719358428538No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA5960.3042461739512185No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA5430.27719072559649605No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC5200.2654496819708618No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG5140.2623868010250442No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA4920.25115623755704614No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC4570.23328943203977665No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC4490.2292055907786864No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA4460.22767415030577762No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT4360.2225693487294149No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC4280.21848550746832468No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT4110.20980734478850807No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA4090.20878638447323553No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG4080.20827590431559928No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA4080.20827590431559928No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA4080.20827590431559928No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT4000.20419206305450904No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG4000.20419206305450904No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG3880.1980663011628738No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA3860.19704534084760125No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG3830.19551390037469246No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT3820.19500342021705616No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG3800.19398245990178362No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT3780.19296149958651107No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT3690.18836717816778462No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3600.18377285674905816No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG3590.1832623765914219No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA3580.18275189643378564No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3540.18070997580324052No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC3510.1791785353303317No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC3510.1791785353303317No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT3480.1776470948574229No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC3450.1761156543845141No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3370.1720318131234239No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC3230.16488509091651607No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT3210.16386413060124352No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG3110.1587593290248808No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA3100.15824884886724452No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC3090.15773836870960825No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG3060.15620692823669946No Hit
GACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTC3010.15365452744851807No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC3000.1531440472908818No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2970.15161260681797298No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2950.15059164650270046No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA2910.14854972587215534No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA2770.14140300366524752No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC2760.14089252350761125No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG2760.14089252350761125No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG2730.13936108303470243No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA2650.13527724177361225No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC2640.13476676161597598No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC2590.1322143608277946No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG2540.12966196003961328No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG2520.1286409997243407No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA2510.12813051956670443No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG2500.12762003940906816No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA2480.1265990790937956No Hit
GGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTC2480.1265990790937956No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA2410.12302571799034173No Hit
GTCTGCATGACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACAT2400.12251523783270543No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT2400.12251523783270543No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA2400.12251523783270543No Hit
ATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGACA2370.12098379735979661No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA2350.11996283704452408No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA2310.11792091641397899No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC2290.11689995609870646No Hit
GACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG2260.11536851562579761No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2260.11536851562579761No Hit
AAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT2240.11434755531052508No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT2230.11383707515288881No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT2230.11383707515288881No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT2220.11332659499525254No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA2210.11281611483761626No Hit
GTGTGTGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC2200.11230563467997999No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT2200.11230563467997999No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2180.11128467436470744No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA2160.11026371404943491No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA2160.11026371404943491No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2150.10975323389179864No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2140.10924275373416234No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA2130.10873227357652607No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC2120.1082217934188898No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC2110.10771131326125354No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC2100.10720083310361726No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC2100.10720083310361726No Hit
CGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAAGAT2070.10566939263070844No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT2070.10566939263070844No Hit
CCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACACCATTGCC2050.1046484323154359No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2040.10413795215779964No Hit
TGCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA1990.10158555136961826No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG1990.10158555136961826No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA1990.10158555136961826No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT1980.10107507121198199No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT1960.10005411089670944No Hit
GGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATAT1960.10005411089670944No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC1960.10005411089670944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)