FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171688
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG8360.48692977960020506No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC8260.4811052607054657No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC8070.470038674805461No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT6270.36519733470015375No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT5700.33199757700013977No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC5330.3104468570896044No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG5190.3022925306369694No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA4760.2772470993895904No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC4640.2702576767159033No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC4310.25103676436326355No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG4300.2504543124737897No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC4290.24987186058431574No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA4230.24637714924747217No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4100.23880527468431106No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA3930.2289035925632543No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT3870.2254088812264107No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT3840.22366152555798893No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3750.21841945855272357No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG3660.2131773915474582No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA3470.20211080564745354No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC3380.19686873864218818No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT3320.19337402730534456No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3220.18754950841060528No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC3180.18521970085270956No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC3050.17764782628954848No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2980.17357066306323096No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2960.17240575928428312No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2900.16891104794743955No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA2860.1665812403895438No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC2610.1520199431526956No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG2580.1502725874842738No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2570.14969013559479988No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2520.14677787614743024No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA2500.14561297236848236No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT2490.1450305204790084No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG2490.1450305204790084No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG2480.1444480685895345No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2460.14328316481058664No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA2360.13745864591584736No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC2300.13396393457900377No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT2270.132216578910582No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT2260.13163412702110805No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT2240.1304692232421602No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA2240.1304692232421602No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC2210.1287218675737384No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG2210.1287218675737384No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA2210.1287218675737384No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC2200.12813941568426446No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2170.1263920600158427No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT2160.12580960812636877No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA2150.12522715623689484No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT2110.12289734867899911No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT2100.12231489678952519No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG2090.12173244490005126No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC2080.12114999301057733No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA2070.1205675411211034No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC2060.11998508923162948No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT2050.11940263734215553No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2050.11940263734215553No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC2040.11882018545268161No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT2030.11823773356320767No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC2010.11707282978425983No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA2010.11707282978425983No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2010.11707282978425983No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT1990.11590792600531195No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT1990.11590792600531195No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA1980.11532547411583803No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT1980.11532547411583803No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA1960.11416057033689017No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA1930.11241321466846839No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT1920.11183076277899447No Hit
CCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATA1880.10950095522109873No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1850.10775359955267694No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC1850.10775359955267694No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT1840.10717114766320301No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC1830.1065886957737291No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG1820.10600624388425516No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA1820.10600624388425516No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC1800.10484134010530731No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA1800.10484134010530731No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG1790.10425888821583337No Hit
GCATGAGGACATGCTGCCGTTACACCTTTGTTCGAGTCATGATTGGGCCA1780.10367643632635945No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG1740.10134662876846372No Hit
GTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATAC1720.10018172498951587No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA1720.10018172498951587No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG1720.10018172498951587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)