FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix09_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix09_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149648
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG7630.509863145514808No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC6890.46041377098257247No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC6790.45373142307281084No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4950.3307762215331979No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT4920.3287715171602694No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG4520.3020421255212231No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC4290.2866727253287715No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT4100.27397626430022454No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3840.25660215973484446No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT3800.2539292205709398No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3780.2525927509889875No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA3770.2519245161980113No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC3770.2519245161980113No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG3540.23655511600555973No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT3290.2198492462311558No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3180.21249866353041805No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT3150.21049395915748959No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA3120.2084892547845611No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC3040.20314337645675182No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC3030.20247514166577568No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2930.1957927937560141No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2720.18175986314551482No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT2630.1757457500267294No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC2580.17240457607184861No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC2570.17173634128087245No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG2550.17039987169892012No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT2440.1630492889981824No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC2420.16171281941623009No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA2390.1597081150433016No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC2320.1550304715064685No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC2300.1536940019245162No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2250.1503528279696354No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2240.14968459317865923No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA2240.14968459317865923No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC2200.14701165401475463No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2190.14634341922377847No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA2140.14300224526889768No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG2120.14166577568694536No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT2080.13899283652304076No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2060.13765636694108843No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2050.13698813215011227No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA2020.1349834277771838No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT1980.13231048861327915No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA1960.13097401903132683No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC1960.13097401903132683No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG1960.13097401903132683No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG1960.13097401903132683No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1950.1303057842403507No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC1940.12963754944937453No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT1880.12562814070351758No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG1860.12429167112156528No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA1860.12429167112156528No Hit
CTTTACCTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTT1820.12161873195766064No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA1820.12161873195766064No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG1810.12095049716668448No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA1800.12028226237570831No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC1790.11961402758473218No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT1750.11694108842082755No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT1750.11694108842082755No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT1740.11627285362985139No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA1740.11627285362985139No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA1720.11493638404789908No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA1690.11293167967497059No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC1680.11226344488399444No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG1680.11226344488399444No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC1670.11159521009301827No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT1660.11092697530204211No Hit
CTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGATGGTGATAAC1650.11025874051106598No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC1650.11025874051106598No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC1640.10959050572008983No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC1640.10959050572008983No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC1630.10892227092911366No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1620.1082540361381375No Hit
CTTCTTTCCCTTTATCATTAATGTAGGATTTGCTGAGCTTTGGGTATGAA1620.1082540361381375No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC1610.10758580134716134No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT1610.10758580134716134No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG1610.10758580134716134No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA1610.10758580134716134No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT1600.10691756655618519No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG1590.10624933176520902No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC1560.10424462739228055No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1540.10290815781032824No Hit
GGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATG1540.10290815781032824No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG1530.10223992301935207No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT1520.10157168822837591No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA1510.10090345343739976No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA1500.10023521864642362No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA1500.10023521864642362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)