FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix01_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix01_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277638
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG13970.503173196752606No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC12400.446624741569958No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC11580.41708987962742855No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT9970.3591006994719743No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC9210.33172692498865425No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT7370.2654535762395637No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT6920.2492454202954927No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA6830.2460037891066785No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA6820.24564360786347691No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA6760.2434825204042674No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT6400.23051599564901057No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG6360.22907527067620426No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC6350.2287150894330027No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC6260.22547345824418846No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC6230.22439291451458376No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG6050.21790965213695532No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA5710.20566348986810162No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA5680.20458294613849687No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA5580.20098113370648113No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC5570.20062095246327952No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG5410.19485805257205424No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC4980.17937025911438637No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT4840.17432772170956426No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC4800.17288699673675795No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC4790.17252681549355636No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA4720.1700055467911453No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT4700.16928518430474213No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC4650.16748427808873426No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC4580.1649630093863232No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC4170.15019557841505846No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG4080.14695394722624425No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC4070.14659376598304266No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA4030.14515304101023635No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3960.14263177230782528No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT3910.14083086609181739No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT3810.13722905365980162No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC3750.13506796620059214No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA3730.13434760371418897No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA3690.13290687874138268No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG3680.13254669749818107No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA3640.1311059725253748No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA3610.13002542879577003No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA3600.12966524755256845No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG3570.12858470382296372No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT3540.12750416009335896No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA3520.12678379760695582No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT3520.12678379760695582No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT3500.12606343512055265No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA3480.1253430726341495No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG3440.12390234766134318No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT3360.12102089771573055No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG3350.12066071647252898No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC3340.1203005352293274No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC3320.11958017274292423No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG3230.11633854155411003No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA3220.11597836031090845No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC3200.11525799782450528No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG3180.11453763533810213No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC3170.11417745409490056No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT3160.11381727285169899No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC3140.1130969103652958No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA3090.11129600414928793No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC3090.11129600414928793No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG3070.11057564166288476No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT3060.11021546041968318No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG3050.10985527917648161No Hit
GTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTCTAGA3050.10985527917648161No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA3040.10949509793328004No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT3030.10913491669007845No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT3030.10913491669007845No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT3010.10841455420367528No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA3000.10805437296047371No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG2990.10769419171727214No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG2960.10661364798766738No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA2910.1048127417716595No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA2850.10265165431245003No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC2840.10229147306924843No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA2820.10157111058284529No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC2810.10121092933964371No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG2810.10121092933964371No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA2800.10085074809644214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)