FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Mix01_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Mix01_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212298
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG10220.48139878849541684No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC8280.3900178051606704No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC7760.36552393333898575No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC7740.3645818613458441No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA7240.3410300615173012No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG6330.2981657858293531No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA6270.29533956984992793No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC6210.2925133538705028No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG6040.28450574192879824No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT5960.28073745395623134No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT5790.27272984201452677No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC5260.24776493419627127No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG4800.22609727835401183No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA4750.22374209837115752No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC4640.2185607024088781No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA4420.2081979104843192No Hit
ATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGACA4350.20490065850832323No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC4290.20207444252889809No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG4260.2006613345391855No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC4250.20019029854261464No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA4190.1973640825631895No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA4130.19453786658376432No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA4100.19312475859405176No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA4050.19076957861119745No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC3920.18464611065577632No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA3860.18181989467635115No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT3850.18134885867978032No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG3810.1794647146934969No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3760.17710953471064259No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC3750.17663849871407172No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT3600.16957295876550887No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA3570.1681598507757963No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG3560.16768881477922543No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA3550.16721777878265456No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC3530.16627570678951284No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3440.16203638282037514No Hit
GGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTC3420.1610943108272334No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG3340.15732602285466654No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA3330.1568549868580957No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG3320.15638395086152485No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA3290.15497084287181226No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG3270.15402877087867056No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC3250.15308669888552884No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA3220.15167359089581625No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT3220.15167359089581625No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT3200.15073151890267453No Hit
GGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATAT3170.14931841091296197No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG3140.14790530292324938No Hit
ACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCTAC3140.14790530292324938No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC3110.14649219493353682No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3090.1455501229403951No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC3000.1413107989712574No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2990.1408397629746865No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2990.1408397629746865No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA2980.14036872697811567No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT2950.13895561898840308No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT2940.13848458299183225No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC2890.13612940300897794No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2880.13565836701240708No Hit
GTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATG2850.13424525902269452No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC2830.1333031870295528No Hit
AGCACGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGA2820.13283215103298193No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC2780.1309480070466985No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2770.13047697105012765No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT2720.12812179106727337No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC2710.1276507550707025No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT2690.1267086830775608No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2670.12576661108441908No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT2670.12576661108441908No Hit
CTCCATGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACAT2660.1252955750878482No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG2580.12152728711528134No Hit
GACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG2530.11917210713242707No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC2520.11870107113585619No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC2510.11823003513928534No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA2490.11728796314614362No Hit
GCATTGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCCC2460.11587485515643105No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA2420.11399071117014763No Hit
AAAGAGAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGG2410.11351967517357676No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT2390.11257760318043505No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA2370.11163553118729334No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC2360.11116449519072247No Hit
GACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTC2350.11069345919415162No Hit
ACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTT2330.1097513872010099No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC2310.1088093152078682No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC2300.10833827921129732No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT2300.10833827921129732No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA2290.10786724321472647No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2290.10786724321472647No Hit
AGAGAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGA2270.10692517122158475No Hit
TGCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA2230.10504102723530133No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA2220.10456999123873045No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA2220.10456999123873045No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG2220.10456999123873045No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG2210.1040989552421596No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA2200.10362791924558876No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG2200.10362791924558876No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2190.10315688324901788No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT2160.10174377525930531No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2160.10174377525930531No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT2150.10127273926273446No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG2150.10127273926273446No Hit
GGATATCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCC2150.10127273926273446No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT2140.1008017032661636No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT2140.1008017032661636No Hit
TTACAAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGA2140.1008017032661636No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG2130.10033066726959275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)