FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n02_SynCon_Control05_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n02_SynCon_Control05_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92105
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG4940.5363443895553988No Hit
CTATAGAACAATTCGACATTAATTGATGGCCGCCCGAATTCTTTTGGTCG4360.4733727810650888No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3640.3952011291460833No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC3250.35285815102328866No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA2830.3072580207372021No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC2620.2844579555941588No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2470.2681721947776994No Hit
ATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCC2310.2508007165734759No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC2300.24971499918571197No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC2280.24754356441018402No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG2180.2366863905325444No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT2080.22582921665490474No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG2060.2236577818793768No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA2000.217143477552793No Hit
ATCTACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATG1990.21605776016502903No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA1910.20737202106291733No Hit
TTCCAATTGTGATCGGATGTATATTATGAAATGGGAGGCTGGTGTTTATA1900.20628630367515335No Hit
GAATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA1860.2019434341240975No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA1820.19760056457304165No Hit
TACTAGTGCTGACCAACAAAGCCTCTATCAGAATGCAGATGCATATGTTT1780.19325769502198578No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA1740.18891482547092991No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA1730.18782910808316594No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA1700.18457195591987405No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC1700.18457195591987405No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT1680.18240052114434613No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA1640.17805765159329026No Hit
CTATAGATGCTTCTGAAATCCTAATGTTAAATACATATTCTACACTGTAG1640.17805765159329026No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC1620.17588621681776234No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA1600.1737147820422344No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA1590.17262906465447045No Hit
TCTCAAAACCGTTCCTTGAACTAATGCTTTTAGTTCTCCCTATCCAAACA1590.17262906465447045No Hit
ACACTGTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG1570.1704576298789425No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT1560.16937191249117856No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC1540.1672004777156506No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA1450.15742902122577493No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG1430.155257586450247No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA1430.155257586450247No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTTCCATTGCGAA1420.15417186906248304No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC1410.15308615167471906No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA1400.15200043428695512No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA1400.15200043428695512No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG1370.14874328212366322No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG1370.14874328212366322No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG1370.14874328212366322No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA1370.14874328212366322No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC1340.1454861299603713No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT1340.1454861299603713No Hit
GTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAACA1310.1422289777970794No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA1290.1400575430215515No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA1290.1400575430215515No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC1270.13788610824602357No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG1270.13788610824602357No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT1270.13788610824602357No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTATT1260.1368003908582596No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC1240.13462895608273168No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG1240.13462895608273168No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA1230.1335432386949677No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT1220.13245752130720373No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC1220.13245752130720373No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG1220.13245752130720373No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT1220.13245752130720373No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA1180.1281146517561479No Hit
GTTAAAAAAGAGAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGA1180.1281146517561479No Hit
GTCTAAGGATATCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC1180.1281146517561479No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT1160.12594321698061994No Hit
GATAATACCAGATCCAGCGTTTCTTTCCATTGCGAATGCATATCTCGGTA1150.12485749959285598No Hit
GTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGA1150.12485749959285598No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG1110.12051463004180013No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA1100.11942891265403616No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT1100.11942891265403616No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC1090.1183431952662722No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAGGTT1090.1183431952662722No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAG1090.1183431952662722No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG1090.1183431952662722No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA1070.11617176049074425No Hit
ATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGACA1070.11617176049074425No Hit
GTTAAATACATATTCTACACTGTAGAGACCAATTAGAGCACATCCAGAAA1060.11508604310298029No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC1060.11508604310298029No Hit
CAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCTT1050.11400032571521634No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGTTTTAC1040.11291460832745237No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCGTTTCTTT1040.11291460832745237No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGCTAAAAAAC1030.1118288909396884No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA1030.1118288909396884No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT1030.1118288909396884No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC1020.11074317355192444No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT1020.11074317355192444No Hit
GCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAGT990.10748602138863253No Hit
TCTCAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATT990.10748602138863253No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA990.10748602138863253No Hit
ATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGTGC990.10748602138863253No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA980.10640030400086858No Hit
CCTCATAGTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTT980.10640030400086858No Hit
AAATAGGCCTCTAGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCA980.10640030400086858No Hit
GAATAGACGGGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTT970.10531458661310461No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA970.10531458661310461No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT960.10422886922534065No Hit
GAATAGAAAAGGGAAAGATAGTCAAATCAGTCGAAATGAATGCCCCTAAT950.10314315183757668No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC950.10314315183757668No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC950.10314315183757668No Hit
AGATTGAATAGACGGGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTG940.1020574344498127No Hit
GGATATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATAT940.1020574344498127No Hit
AATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTTCTATT930.10097171706204874No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG930.10097171706204874No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)