FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix43_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix43_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256685
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC12580.4900948633539163No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC12220.47606989111167386No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG11380.44334495587977485No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC11070.4312678964489549No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC10220.3981533786547714No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT9860.384128406412529No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT8800.3428326548103707No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT8100.31556187545045483No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC7900.30777022420476463No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC7870.3066014765179111No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA7870.3066014765179111No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA7610.29647232989851374No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA7140.2781619494711417No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT6780.26413697722889923No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC6600.257124491107778No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC6570.2559557434209245No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA6130.23881411068040595No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG6020.2345287024952763No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA5970.23258078968385376No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC5880.22907454662329316No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA5340.20803708825992948No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA5100.19868710676510118No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC4930.1920642032062645No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC4890.19050587295712643No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG4620.1799871437754446No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA4570.17803923096402205No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC4500.17531215302803047No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT4460.1737538227788924No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG4400.17141632740518534No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG4350.16946841459376277No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA4130.16089759822350352No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG4130.16089759822350352No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG4120.160508015661219No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT4120.160508015661219No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG4090.15933926797436548No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT4020.1566121900383739No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT3860.15037886904182168No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG3830.14921012135496814No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC3800.1480413736681146No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT3780.14726220854354558No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT3740.14570387829440754No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC3730.145314295732123No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3710.144535130607554No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA3710.144535130607554No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG3680.14336638292070047No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA3650.14219763523384693No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC3620.1410288875469934No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC3590.13986013986013987No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT3570.13908097473557085No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA3570.13908097473557085No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT3500.13635389679957927No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA3470.13518514911272572No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT3440.13401640142587218No Hit
GACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTC3440.13401640142587218No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA3420.13323723630130316No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA3390.13206848861444961No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA3330.12973099324074255No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG3300.128562245553889No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC3270.12739349786703547No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC3250.12661433274246645No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG3250.12661433274246645No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG3230.12583516761789743No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA3230.12583516761789743No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA3230.12583516761789743No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC3210.12505600249332838No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC3190.12427683736875937No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG3160.12310808968190583No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC3160.12310808968190583No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA3140.12232892455733681No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC3050.1188226814967762No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC3020.11765393380992267No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT3020.11765393380992267No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG2950.11492685587393109No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2940.11453727331164658No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT2940.11453727331164658No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG2930.11414769074936205No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA2920.11375810818707754No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG2910.11336852562479303No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG2850.11103103025108596No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2840.11064144768880145No Hit
CTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGATGGTGATAAC2810.10947270000194792No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2800.10908311743966341No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA2800.10908311743966341No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC2790.1086935348773789No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG2770.10791436975280987No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG2740.10674562206595634No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA2680.10440812669224925No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2680.10440812669224925No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA2660.10362896156768023No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT2640.1028497964431112No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2610.10168104875625766No Hit
GGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTC2600.10129146619397315No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA2570.10012271850711961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)