FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix38_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix38_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107284
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC12791.192162857462436No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA11341.057007568696171No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC7900.7363632974162037No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA7010.6534059132769099No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG6450.6012080086499384No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCC6180.5760411617762201No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA5420.5052011483539018No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA5140.4791021960404161No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAA4700.43808955669065286No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4370.4073300771783304No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT4290.3998732336601916No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT4010.3737742813467059No Hit
GTCTAAGGATATCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC3610.3364900637560121No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3510.3271690093583386No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG3420.3187800604004325No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC3400.3169158495208978No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAAAAGAACC3370.31411953320159575No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAG3350.31225532232206105No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA3090.28802058088811006No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG3090.28802058088811006No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT2980.2777674210506692No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA2880.26844636665299576No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAATTAATTGATGGCCGC2860.26658215577346106No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC2840.2647179448939264No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAGGTT2780.2591253122553223No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC2760.2572611013757876No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT2570.23955109802020805No Hit
CTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTG2530.23582267626113865No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG2500.2330263599418366No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATAT2430.2265016218634652No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT2430.2265016218634652No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAAAAGAACCCC2320.21624846202602438No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC2320.21624846202602438No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC2320.21624846202602438No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA2290.21345214570672233No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATATGGTACCTTCG2280.21252004026695498No Hit
TTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACAGTGGTCTTA2270.21158793482718763No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAA2240.20879161850788563No Hit
ATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2230.20785951306811828No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG2220.20692740762835093No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC2150.20040266954997948No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA2120.19760635323067746No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT2110.1966742477909101No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC2030.18921740427277134No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC2020.188285298833004No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT2010.18735319339323664No Hit
ATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA2010.18735319339323664No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCCCTCCCGCTTACTATCAACTG2010.18735319339323664No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC2000.1864210879534693No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA1960.18269266619439992No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC1960.18269266619439992No Hit
CTCTTATCCCTCTCAGCGACATCTCCATGCTTGGGGTCATGTCGGGCAGT1930.17989634987509787No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT1930.17989634987509787No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA1920.17896424443533052No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA1920.17896424443533052No Hit
CATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAATGAAGTGGATGATG1890.1761679281160285No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG1870.1743037172364938No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA1850.17243950635695912No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA1810.16871108459788972No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA1810.16871108459788972No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCAC1810.16871108459788972No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTCTTGACTGATGATCC1800.16777897915812237No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC1790.16684687371835502No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCT1770.16498266283882032No Hit
GATTGGAGGAGTAAGGATGGTGGATATCCTTAGACAGAATCCAACTGAGG1770.16498266283882032No Hit
ATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGC1730.16125424107975095No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA1690.15752581932068155No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG1690.15752581932068155No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC1660.15472950300137953No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT1660.15472950300137953No Hit
TGCTGGAACAGTGTCCTTACAAATCCACTGTACCGGCTTCTGGTTGCCTT1660.15472950300137953No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG1650.15379739756161218No Hit
AATTAGCACATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA1640.15286529212184483No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA1630.15193318668207748No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAGTAGGATG1620.15100108124231013No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT1620.15100108124231013No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG1620.15100108124231013No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG1610.15006897580254278No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCATCCGAATTCTTTTATTC1610.15006897580254278No Hit
CAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGT1600.14913687036277545No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA1590.1482047649230081No Hit
GTTCTTTTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGT1580.14727265948324075No Hit
GTGTCCTTACAAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA1580.14727265948324075No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA1580.14727265948324075No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG1570.1463405540434734No Hit
GTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTAGATCTCTCAG1570.1463405540434734No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGATGTAC1570.1463405540434734No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAAT1570.1463405540434734No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT1560.14540844860370605No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC1540.14354423772417135No Hit
GAAAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC1540.14354423772417135No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT1500.13981581596510195No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT1480.13795160508556728No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1480.13795160508556728No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC1470.13701949964579993No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA1460.13608739420603258No Hit
CAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG1450.13515528876626523No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC1450.13515528876626523No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA1450.13515528876626523No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT1430.13329107788673053No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG1430.13329107788673053No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA1420.1323589724469632No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT1410.13142686700719586No Hit
GAACTGAGAGGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC1400.1304947615674285No Hit
ACTTCATTCTGAGTGCGGGGTTCTTTTCTTGCCTTCCTGATGTGTACTTT1400.1304947615674285No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA1390.12956265612766116No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTCTT1390.12956265612766116No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC1390.12956265612766116No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAG1380.1286305506878938No Hit
GTAATTGGGTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT1370.12769844524812646No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT1370.12769844524812646No Hit
ACATTAGATCTCTCAGTTCTTTTATTCTCTCCATATTGAATATAATTGAC1330.12397002348905709No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA1330.12397002348905709No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA1320.12303791804928974No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT1320.12303791804928974No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT1310.12210581260952239No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT1300.12117370716975505No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG1300.12117370716975505No Hit
ACAATTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA1300.12117370716975505No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAAGAGGATTGCATGATCAAGG1280.11930949629022035No Hit
GTTTATATTGAAGTGTTGCACTTAACCCAAGGAACGTGCTGGGAGCAGAT1270.118377390850453No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT1260.11744528541068565No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGC1260.11744528541068565No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGGCGGCC1260.11744528541068565No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT1260.11744528541068565No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAAGAGGATT1250.1165131799709183No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATATCCACCATCC1250.1165131799709183No Hit
GTACACATCAGGAAGGCAAGAAAAGAACCCCGCACTCAGAATGAAGTGGA1240.11558107453115098No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT1240.11558107453115098No Hit
GCCTTGATCATGCAATCCTCTTGTGAGAATACCATGGCCACAATTATTGC1230.11464896909138363No Hit
GTCCACAGTGGTCTTAGTGAGTATCTCGCGAGTGCGGGACTGCGACATTA1220.11371686365161628No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA1210.11278475821184893No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAAAA1210.11278475821184893No Hit
GATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATT1200.11185265277208158No Hit
GTGTACATCTGCTCCCAGCACGTTCCTTGGGTTAAGTGCAACACTTCAAT1190.11092054733231423No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA1190.11092054733231423No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC1190.11092054733231423No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT1190.11092054733231423No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATATCCA1190.11092054733231423No Hit
GTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTG1180.10998844189254689No Hit
CTCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT1170.10905633645277954No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTT1170.10905633645277954No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT1160.10812423101301219No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC1160.10812423101301219No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG1150.10719212557324484No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT1150.10719212557324484No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT1130.10532791469371014No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCATTC1120.10439580925394282No Hit
GTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGT1120.10439580925394282No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG1120.10439580925394282No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC1110.10346370381417547No Hit
GCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTT1110.10346370381417547No Hit
GGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAATATGGTACCT1110.10346370381417547No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG1110.10346370381417547No Hit
ATATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGA1110.10346370381417547No Hit
TTTCTTGCCTTCCTGATGTGTACTTTTTGATTATGGCCATATGGTCCACA1110.10346370381417547No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT1100.10253159837440812No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAGAATTGCATCC1100.10253159837440812No Hit
CTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCCAAT1090.10159949293464077No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAAAAGA1080.10066738749487342No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)