FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix37_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix37_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71464
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC5800.8115974476659576No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC5570.7794134109481697No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC5300.7416321504533752No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4170.5835105787529385No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA3730.5219411172058659No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA3690.5163438934288593No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3440.481361244822568No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA3140.4393820664950185No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT3130.43798276055076685No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT2980.41699317138699205No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2970.4155938654427404No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA2840.39740288816746894No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2590.36242023956117764No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC2580.361020933616926No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA2530.35402440389566775No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT2290.3204410612336281No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1990.2784618829060786No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT1990.2784618829060786No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG1930.27006604724056865No Hit
CTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATC1910.2672674353520654No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT1890.2644688234635621No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG1880.2630695175193104No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC1860.2602709056308071No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA1780.24907645807679393No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA1720.240680622411284No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT1660.2322847867457741No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC1560.21829172730325758No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCAC1490.20849658569349602No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1480.20709727974924438No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG1470.20569797380499272No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT1430.20010075002798614No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT1410.19730213813948283No Hit
CTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTA1410.19730213813948283No Hit
CAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGC1410.19730213813948283No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA1410.19730213813948283No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG1390.1945035262509795No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA1380.19310422030672786No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG1360.19030560841822455No Hit
GATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGG1340.18750699652972128No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA1320.18470838464121794No Hit
GAGTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGG1290.180510466808463No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG1280.17911116086421133No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC1280.17911116086421133No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA1260.17631254897570806No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT1260.17631254897570806No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC1250.1749132430314564No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC1240.17351393708720475No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC1240.17351393708720475No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA1230.1721146311429531No Hit
TCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAGACAATAGTACGTTC1230.1721146311429531No Hit
GAACTGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC1210.16931601925444978No Hit
ACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTG1190.1665174073659465No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT1150.1609201835889399No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT1120.15672226575618492No Hit
CCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTT1110.15532295981193328No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA1110.15532295981193328No Hit
ATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTG1110.15532295981193328No Hit
GTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCT1110.15532295981193328No Hit
GCCTTGATCATGCAATCCTCTTGTGAGAATACCATGGCCACAATTATTGC1100.15392365386768164No Hit
CCTCCAAACACTGAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAA1090.15252434792342998No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT1080.15112504197917834No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1080.15112504197917834No Hit
GGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCCCCTAAC1070.14972573603492667No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATT1070.14972573603492667No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG1050.14692712414642337No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA1050.14692712414642337No Hit
CCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTC1040.14552781820217173No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC1040.14552781820217173No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT1040.14552781820217173No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC1030.14412851225792006No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA1030.14412851225792006No Hit
GTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTACTATTG1030.14412851225792006No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAG1030.14412851225792006No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC1020.14272920631366842No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1020.14272920631366842No Hit
CTACCACTCTCTCCGTGCTGGAGTATTCATCTACTCCCATTTTGCTGACT1010.14132990036941676No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA1010.14132990036941676No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC1010.14132990036941676No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT1010.14132990036941676No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG1000.13993059442516512No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG1000.13993059442516512No Hit
CAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAA1000.13993059442516512No Hit
GGTTGGGAGAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGA990.13853128848091348No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAAGAGGATT980.1371319825366618No Hit
TCCTTACTCCTCCAATCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCT980.1371319825366618No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT980.1371319825366618No Hit
GTGGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAA960.1343333706481585No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT960.1343333706481585No Hit
ATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAGTTGTC950.13293406470390687No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT940.1315347587596552No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT930.13013545281540356No Hit
GTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGAGA920.12873614687115192No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG920.12873614687115192No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC920.12873614687115192No Hit
CCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAG920.12873614687115192No Hit
GTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGGGCCAT910.12733684092690026No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA910.12733684092690026No Hit
CTTTTATTCTCTCCATATTGAATATAATTGACCTGCTTTTGCTCCTGTCT900.1259375349826486No Hit
AGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGTAC900.1259375349826486No Hit
GTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTTAGGGACAAGA900.1259375349826486No Hit
GTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCC900.1259375349826486No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG890.12453822903839697No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAATGTGCTAATT890.12453822903839697No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC890.12453822903839697No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAAGA880.12313892309414531No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA870.12173961714989365No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGCTGTCT870.12173961714989365No Hit
ACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCCTACTC870.12173961714989365No Hit
ATTATATTCAATATGGAGAGAATAAAAGAATTCGGATGGCCATCAATTAG870.12173961714989365No Hit
GGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAG870.12173961714989365No Hit
ACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAG870.12173961714989365No Hit
CTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTC860.120340311205642No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT860.120340311205642No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT860.120340311205642No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA860.120340311205642No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC850.11894100526139036No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG850.11894100526139036No Hit
GTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATTTCTCACTT850.11894100526139036No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAGTAGGATG840.1175416993171387No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT840.1175416993171387No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCGTTCTCTCCTTTTGCAA840.1175416993171387No Hit
GCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTCCT830.11614239337288705No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAAGAGGATTGCATGATCAAGG830.11614239337288705No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAACGCTAA830.11614239337288705No Hit
GTTTTATATACCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCT830.11614239337288705No Hit
CATTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT820.11474308742863538No Hit
GAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAG820.11474308742863538No Hit
GATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATT820.11474308742863538No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA820.11474308742863538No Hit
GAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA810.11334378148438376No Hit
CTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCA810.11334378148438376No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC800.1119444755401321No Hit
GTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGC790.11054516959588044No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA790.11054516959588044No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTAC780.10914586365162879No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT780.10914586365162879No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC770.10774655770737715No Hit
GCTAGAAACATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATC770.10774655770737715No Hit
CTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTG770.10774655770737715No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA770.10774655770737715No Hit
CAATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAAC770.10774655770737715No Hit
GCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTT750.10494794581887383No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCGGA750.10494794581887383No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC740.10354863987462219No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC740.10354863987462219No Hit
ACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGG740.10354863987462219No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC740.10354863987462219No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC740.10354863987462219No Hit
GATCAAGGCAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGC730.10214933393037054No Hit
GTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAA730.10214933393037054No Hit
CCTTAGGGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCAT730.10214933393037054No Hit
GTTCTGGTGGAGCAGCAGCAAAGGGGAGAAGTTTTATTATTTGGACAGTG730.10214933393037054No Hit
GGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTCCTGATTATCCA730.10214933393037054No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC720.10075002798611889No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC720.10075002798611889No Hit
ATTGATAAGTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATC720.10075002798611889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)