FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix35_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix35_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103523
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC9710.9379558165818224No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA7830.7563536605392038No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC6570.6346415772340446No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC5220.5042357736928026No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA4800.46366507925774947No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC4760.4598012035972683No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG4580.4424137631251026No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4520.4366179496343808No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT4360.4211624469924558No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC4030.3892854727934855No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC3650.3525786540189137No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG3590.3467828405281918No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3480.3361571824618684No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT3330.3216676487350637No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3320.3207016798199434No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT3320.3207016798199434No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA3290.31780377307458246No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA3160.30524617717801844No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG3150.30428020826289814No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3010.29075664345121377No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG2820.27240323406392786No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG2810.2714372651488075No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2740.2646754827429653No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA2710.26177757599760443No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA2570.24825401118592003No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCAT2290.2212068815625513No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA2190.21154719241134823No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC2160.20864928566598726No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT2150.20768331675086693No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA2140.20671734783574663No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2140.20671734783574663No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2130.20575137892062634No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG2110.20381944109038572No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2040.19705765868454356No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT2010.19415975193918258No Hit
AGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTG1900.18353409387285918No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC1880.18160215604261853No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC1840.1777382803821373No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT1840.1777382803821373No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA1840.1777382803821373No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC1820.1758063425518967No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA1810.17484037363677637No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG1770.17097649797629513No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA1770.17097649797629513No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG1730.1671126223158139No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA1730.1671126223158139No Hit
AAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCA1710.16518068448557324No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT1710.16518068448557324No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC1700.16421471557045295No Hit
GGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG1700.16421471557045295No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT1700.16421471557045295No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA1670.161316808825092No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA1670.161316808825092No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG1670.161316808825092No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG1650.1593848709948514No Hit
GTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATAC1610.15552099533437014No Hit
CTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTG1600.15455502641924984No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT1570.1516571196738889No Hit
ATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTC1560.15069115075876857No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA1530.14779324401340765No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT1510.14586130618316703No Hit
CCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATA1500.1448953372680467No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC1490.1439293683529264No Hit
TCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT1460.14103146160756547No Hit
GAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTT1460.14103146160756547No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT1450.14006549269244517No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT1430.13813355486220455No Hit
TTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAG1420.13716758594708423No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC1400.1352356481168436No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC1390.13426967920172328No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT1390.13426967920172328No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT1380.13330371028660298No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA1370.13233774137148266No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC1360.13137177245636236No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG1340.12943983462612174No Hit
CTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATACAG1340.12943983462612174No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG1310.1265419278807608No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA1290.12460999005052018No Hit
ATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGC1280.12364402113539986No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA1280.12364402113539986No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC1270.12267805222027955No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC1270.12267805222027955No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA1260.12171208330515924No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA1250.12074611439003893No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA1250.12074611439003893No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAG1240.11978014547491861No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT1230.1188141765597983No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA1230.1188141765597983No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAAC1220.11784820764467799No Hit
ACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCAT1220.11784820764467799No Hit
CACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACA1210.11688223872955768No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT1210.11688223872955768No Hit
GTTAGTAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCAT1200.11591626981443737No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC1200.11591626981443737No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA1190.11495030089931707No Hit
GTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGC1190.11495030089931707No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC1190.11495030089931707No Hit
TGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCAAATGA1190.11495030089931707No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC1180.11398433198419676No Hit
TTCACCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCA1180.11398433198419676No Hit
CCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTC1170.11301836306907645No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA1170.11301836306907645No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA1160.11205239415395611No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC1150.1110864252388358No Hit
TCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCT1120.10818851849347488No Hit
GTGCTATGGGGCATTCACCATCCATCTACTAGTGCTGACCAACAAAGTCT1120.10818851849347488No Hit
GGTAAGAAGCCAGTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCT1120.10818851849347488No Hit
ACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAGTCCTC1110.10722254957835457No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG1110.10722254957835457No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT1100.10625658066323426No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA1100.10625658066323426No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG1090.10529061174811395No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC1090.10529061174811395No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG1080.10432464283299363No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT1080.10432464283299363No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC1070.10335867391787332No Hit
GGTGTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGA1070.10335867391787332No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA1070.10335867391787332No Hit
ACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTA1070.10335867391787332No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA1060.102392705002753No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC1050.1014267360876327No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG1050.1014267360876327No Hit
CTCTTAGCTCCTCATAATCGATGAAATCTCCTGGGTAACACGTTCCATTG1050.1014267360876327No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC1040.10046076717251239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)