FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix25_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix25_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319320
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC18000.5636978579481398No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG16890.5289364900413379No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA14860.46536389828385316No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC14610.45753476136790683No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC12760.39959914818990355No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT11840.37078792433922086No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC10690.3347738945258675No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC10420.32631842665664534No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA9740.3050231742452712No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA9650.3022046849555305No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT9500.29750720280596266No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA9230.2890517349367406No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA8500.26619065514217716No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG7940.24865338845045723No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG7770.24332957534761368No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA7720.24176374796442437No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG7560.23675310033821872No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT7310.2289239634222723No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA7180.22485281222598022No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC7130.22328698484279091No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG7020.21984216459977451No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA7000.21921583364649883No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG6970.21827633721658524No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT6920.216710509833396No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA6700.20982086934736316No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA6680.20919453839408744No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG6670.20888137291744957No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG6620.20731554553426032No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC6580.2060628836277089No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTCCTTGA6530.2044970562445196No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA6440.20167856695477887No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA6310.19760741575848678No Hit
GCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGT6300.1972942502818489No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC6280.19666791932857322No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT6240.19541525742202182No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG6190.1938494300388325No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC6170.1932230990855568No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG6080.1904046097958161No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC6050.18946511336590255No Hit
CTAATGAGCTGTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATT5970.1869597895527997No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA5880.184141300263059No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC5710.17881748716021545No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT5700.1785043216835776No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA5660.1772516597770262No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC5660.1772516597770262No Hit
ATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTT5650.17693849430038833No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG5630.1763121633471126No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC5620.17599899787047477No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA5370.16816986095452838No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA5350.16754353000125266No Hit
CTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTGT5340.16723036452461482No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC5320.1666040335713391No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA5260.16472504071151198No Hit
GTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC5110.16002755856194414No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT5100.15971439308530627No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA5060.15846173117875484No Hit
GGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTC5000.15658273831892772No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC4800.1503194287861706No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA4790.15000626330953276No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG4680.14656144306651636No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC4610.14436928473005134No Hit
ACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCTAC4590.14374295377677565No Hit
GATGTATATTATGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC4550.14249029187022424No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG4530.14186396091694853No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT4480.14029813353375922No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT4460.1396718025804835No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA4450.13935863710384566No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT4390.13747964424401854No Hit
GGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATG4360.13654014781410498No Hit
GTAGAGACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT4300.13466115495427783No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC4270.1337216585243643No Hit
TTTTTAAACTATTCGACAATTAATTGATGGCCGCCCGAATTCTTTTGGTC4260.13340849304772642No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA4180.13090316923462356No Hit
ATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGACA4120.12902417637479646No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC4090.12808467994488287No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC4060.1271451835149693No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGGAGGCAATACTAGT4040.1265188525616936No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC4010.12557935613178003No Hit
AAAGACAACAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAG4000.1252661906551422No Hit
GACCAATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG3990.12495302517850432No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG3930.1230740323186772No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA3820.1196292120756608No Hit
GTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCC3810.11931604659902292No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA3790.1186897156457472No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC3710.11618439183264437No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3710.11618439183264437No Hit
GATTACTTGTCAATGGTAAATGGCAACTCAGCACCGTCCGGCCAAGACCA3680.11524489540273081No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA3670.11493172992609296No Hit
CTATTGGTACCGAGATATGCATTCGCAATGGAAAGAAACGCTGGATCTGG3670.11493172992609296No Hit
GCATACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCAT3640.11399223349617937No Hit
GTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATG3600.11273957158962795No Hit
GGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATAT3600.11273957158962795No Hit
GTGTTAAAGTACAACGGAATAATAACAGACACTATCAAGAGTTGGAGAAA3590.11242640611299011No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG3580.11211324063635224No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA3570.1118000751597144No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG3570.1118000751597144No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG3570.1118000751597144No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT3550.11117374420643869No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTA3550.11117374420643869No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC3530.11054741325316297No Hit
GTGTCACTGTTTACACCACAAAAAGATATGCTGCTCCCGCTAGTCCAGAT3510.10992108229988726No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGAGA3460.108355254916698No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA3450.10804208944006012No Hit
GTCTGCATGACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACAT3430.1074157584867844No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATATCCTG3380.10584993110359514No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT3380.10584993110359514No Hit
CTCCATGCTTGGGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACAT3370.10553676562695727No Hit
GGATAACAGGAGCATTCCTCATAGTGATAATTAGGGGCATTCATTTCGAC3360.10522360015031942No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTACATATGTATCCTAT3350.10491043467368158No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC3310.10365777276713016No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA3300.10334460729049229No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC3290.10303144181385444No Hit
CCCCTAATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAA3290.10303144181385444No Hit
GACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTC3270.10240511086057874No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA3260.10209194538394087No Hit
GTCTGAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATG3260.10209194538394087No Hit
GCATTGGGTTCAGTCGCTGGTTTGCCCTATTGACAAAGTTCAGATCGCCC3260.10209194538394087No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCGCTATA3260.10209194538394087No Hit
ACCCAGAAGCAAGGTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTG3240.10146561443066515No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT3230.10115244895402731No Hit
GCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATAGTCAAATCAGT3220.10083928347738944No Hit
GAATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACA3200.10021295252411375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)