FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix18_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix18_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151121
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC7700.5095254795825861No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC7640.5055551511702543No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC6870.4546026032119957No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA6700.4433533393770555No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC6630.43872128956266837No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT5970.39504767702701804No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG4800.31762627298654716No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT4190.27726126746117347No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGGACAAAACTCCCG4110.27196749624473104No Hit
ACTCAGCACCGTCCGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA4090.2706440534406204No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG3930.2600565110077355No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC3890.25740962539951434No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC3840.25410101838923776No Hit
TTATTCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAA3730.24682208296662936No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA3570.23623454053374446No Hit
AAGGAAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGG3440.22763216230702552No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC3430.2269704409049702No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC3420.22630871950291487No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC3410.22564699810085956No Hit
ACAACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA3410.22564699810085956No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA3330.2203532268844171No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3300.2183680626782512No Hit
TATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAGGC3260.21572117707002997No Hit
GTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCT3190.2110891272556428No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3160.2091039630494769No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC3110.20579535603920035No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3100.20513363463714507No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA3020.19983986342070262No Hit
GGATACATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2920.19322264940014955No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA2890.19123748519398362No Hit
CTGTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGT2800.1852819925754859No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT2800.1852819925754859No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG2780.18395854977137524No Hit
CAAAAAGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCG2770.18329682836931996No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA2760.18263510696726465No Hit
CAGTGTAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT2720.1799882213590434No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2700.1786647785549328No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2530.1674155147199926No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG2510.16609207191588196No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT2460.16278346490560544No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC2440.16146002210149482No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA2430.1607983006994395No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG2430.1607983006994395No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG2420.16013657929738423No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT2360.15616625088505237No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA2360.15616625088505237No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT2350.15550452948299706No Hit
ATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTCTGACA2350.15550452948299706No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG2320.15351936527683113No Hit
GAATTCTCTTTTTTAACTAGCCATATTAAATTTTTGTAGAAGCTTTTTGC2300.15219592247272054No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC2290.15153420107066523No Hit
GACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAG2270.1502107582665546No Hit
CACTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACC2240.14822559406038868No Hit
GGAGACTTCATCGATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTC2170.14359354424600154No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC2120.14028493723572502No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG2110.1396232158336697No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT2070.13697633022544847No Hit
GACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTC2020.13366772321517195No Hit
CTACATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA1990.13168255900900602No Hit
GGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATAT1980.1310208376069507No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC1920.12705050919461888No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG1900.12572706639050826No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA1900.12572706639050826No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA1860.12308018078228704No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG1850.12241845938023173No Hit
GTGTGTGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC1840.12175673797817643No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA1820.12043329517406581No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG1820.12043329517406581No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA1820.12043329517406581No Hit
GTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATG1790.11844813096789991No Hit
CATATGTAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG1780.11778640956584459No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT1770.11712468816378928No Hit
ACAACTAATTGATGGCCGCCCGAATTCTTTTGGTCGCTGTCTGGCTGTCA1750.11580124535967866No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG1740.11513952395762336No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA1720.11381608115351276No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA1720.11381608115351276No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA1710.11315435975145743No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC1710.11315435975145743No Hit
CCCTTGGAATGCAGAACCTTCTTCTTGACTCAAGGGGCCTTGCTAAATGA1700.11249263834940212No Hit
GTCTGCATGACAATTGGAATGGCTAACTTAATATTACAAATTGGAAACAT1680.11116919554529153No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC1680.11116919554529153No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA1670.11050747414323621No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC1670.11050747414323621No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT1660.1098457527411809No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA1640.10852230993707031No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA1640.10852230993707031No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC1620.10719886713295967No Hit
GTCCTATTGGTGAAGTGCCCTCTCCATACAACTCAAGATTTGAGTCAGTC1600.10587542432884908No Hit
ATTATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA1600.10587542432884908No Hit
ACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCGGCTCTAC1590.10521370292679376No Hit
GCTATATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGA1580.10455198152473845No Hit
ATCCTGACCATTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA1550.10256681731857253No Hit
AGAGAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGA1550.10256681731857253No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG1550.10256681731857253No Hit
AAAGAGAATTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGG1540.10190509591651722No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG1540.10190509591651722No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC1530.10124337451446193No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG1530.10124337451446193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)