FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix07_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix07_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177424
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC10800.6087113355577599No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG10060.5670033366399134No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC9710.547276580394986No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT7920.44638831274235724No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC6700.3776264766886103No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC5850.32971864009378665No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC5840.3291550184867887No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT5580.3145008567048426No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG5500.30999188384885923No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA5350.30153755974389035No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA5070.2857561547479484No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA4980.2806835602849671No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA4900.27617458742898365No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT4830.27222923617999817No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG4610.2598295608260438No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC4350.24517539904409777No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4100.23108485886914962No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG3990.22488502119217244No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3850.21699431869420144No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT3740.21079448101722428No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTGGTC3580.20177653530525747No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT3510.19783118405627195No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC3440.1938858328072865No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA3400.1916313463792948No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG3330.1876859951303093No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA3240.18261340066732798No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC3160.17810442781134458No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA3160.17810442781134458No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG3150.17754080620434665No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA3150.17754080620434665No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT3000.16908648209937777No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG2990.16852286049237983No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2820.1589412931734151No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC2820.1589412931734151No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC2810.15837767156641716No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG2810.15837767156641716No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA2810.15837767156641716No Hit
CCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATG2670.15048696906844622No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT2670.15048696906844622No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT2670.15048696906844622No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC2630.1482324826404545No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC2620.14766886103345658No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA2610.14710523942645864No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT2590.1459779962124628No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2580.14541437460546489No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT2580.14541437460546489No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA2530.14259626657047525No Hit
AGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTG2430.13696005050049598No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA2390.13470556407250428No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC2370.13357832085850843No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG2370.13357832085850843No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2330.13132383443051673No Hit
AAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCA2330.13132383443051673No Hit
TTCATACCCAAAGCTCAGCAAATCCTACATTAATGATAAAGGGAAAGAAG2300.12963296960952295No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT2280.1285057263955271No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT2270.12794210478852916No Hit
CCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATA2250.1268148615745333No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA2240.1262512399675354No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC2240.1262512399675354No Hit
ATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGT2220.12512399675353955No Hit
ATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGCCAGTTAAAAAAC2200.1239967535395437No Hit
ATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTC2160.12174226711155198No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG2160.12174226711155198No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC2150.12117864550455407No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA2140.12061502389755614No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA2130.12005140229055822No Hit
GTCTTGGGGAATATCTCAAACCTTTCAAATGATGACACTGAGCTCAATTG2100.11836053746956444No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA2090.11779691586256649No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG2090.11779691586256649No Hit
GAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTT2070.11666967264857064No Hit
CATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAA2060.11610605104157273No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC2040.11497880782757688No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA2000.11272432139958519No Hit
TCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT1990.11216069979258723No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA1980.11159707818558931No Hit
TTCACCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCA1970.1110334565785914No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT1960.11046983497159348No Hit
GTCTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATAC1950.10990621336459555No Hit
CTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAA1940.10934259175759763No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT1940.10934259175759763No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT1930.1087789701505997No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA1930.1087789701505997No Hit
CTCTATCAGAATGCAGATGCATATGTTTTTGTGGGGACATCAAGATACAG1910.10765172693660385No Hit
AACTATTACTGGACACTAGTAGAGCCGGGAGACAAAATAACATTCGAAGC1910.10765172693660385No Hit
TCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTA1910.10765172693660385No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA1910.10765172693660385No Hit
GTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTAT1890.10652448372260798No Hit
AGTCATGATTGGGCCATGAACTTGTCTTGGGGAATATCTCAAACCTTTCA1890.10652448372260798No Hit
GTTAAAAAACAATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACC1860.10483361890161422No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC1860.10483361890161422No Hit
ACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTCACCAT1860.10483361890161422No Hit
CTTCTTTCCCTTTATCATTAATGTAGGATTTGCTGAGCTTTGGGTATGAA1850.10426999729461629No Hit
ATGCCGAACTGTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCAC1850.10426999729461629No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC1840.10370637568761837No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC1810.10201551086662457No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA1800.10145188925962664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)