FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Mix06_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Mix06_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348479
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG30660.8798234613850476No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC26670.7653258876431578No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT24560.7047770453886748No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC21730.6235669868198657No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC19720.5658877579423724No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT17300.4964431142192212No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC15360.44077261470562074No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA14780.4241288571190803No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA11280.3236923889244402No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC11250.32283150491134327No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA10500.3013094045839204No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC10000.2869613376989718No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG9800.2812221109449924No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG9690.27806553623030367No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG9630.2763437682041099No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA9600.2754828841910129No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA9290.2665870827223448No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT9170.26314354666995715No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG8480.2433432143687281No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC8410.2413344850048353No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA8330.23903879430324354No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT8190.23502133557545793No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTGTAAGAATCGGTTC8000.22956907015917746No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG7540.21636884862502476No Hit
GTAGATGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC7420.21292531257263708No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT7250.20804696983175458No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA7180.2060382404678618No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAACTGA7080.20316862709087202No Hit
CAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAGC6950.1994381297007854No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC6790.19484674829760187No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT6760.19398586428450496No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTCGTCA6740.193411941609107No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG6530.1873857535174286No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT6520.18709879217972963No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT6410.18394221746504094No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG6220.17848995204876048No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT6060.17389857064557693No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA6000.1721768026193831No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT5830.16729845987850056No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG5820.1670114985408016No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT5790.1661506145277047No Hit
GTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG5780.16586365319000573No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA5770.16557669185230675No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC5770.16557669185230675No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA5760.16528973051460777No Hit
CTGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTC5690.16328100115071495No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC5630.16155923312452114No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT5520.15840265840983245No Hit
CCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATG5290.15180254764275608No Hit
GTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTAT5210.14950685694116433No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA5120.14692420490187355No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG5000.1434806688494859No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT4990.14319370751178692No Hit
ATCATGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCA4970.14261978483638899No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG4930.1414719394855931No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA4930.1414719394855931No Hit
AAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCA4860.13946321012170032No Hit
GTGTATGCAGGGATAACTGGCATGGCTCGAATCGACCGTGGGTGTCTTTC4830.1386023261086034No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC4800.13774144209550646No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA4770.13688055808240954No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT4750.1363066354070116No Hit
AGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTG4750.1363066354070116No Hit
GTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTACTA4740.13601967406931265No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA4730.13573271273161366No Hit
CTCCACAGCAAGCTCATGGTCCTACATTGTGGAAACATCTAGTTCAGACA4720.1354457513939147No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG4700.13487182871851677No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG4610.132289176679226No Hit
ATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGTGTT4610.132289176679226No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC4590.13171525400382805No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC4480.1285586792891394No Hit
ACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGA4470.1282717179514404No Hit
GTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCT4280.12281945253515994No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC4240.12167160718436405No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA4220.12109768450896612No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA4220.12109768450896612No Hit
TAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACC4140.11880199380737433No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA4140.11880199380737433No Hit
CTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTG4130.11851503246967537No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA4090.11736718711887947No Hit
GGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATG4080.11708022578118049No Hit
CCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATA4060.11650630310578255No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC4060.11650630310578255No Hit
CCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGTGTATCTGA4060.11650630310578255No Hit
GTGTGAATCACTCTCCACAGCAAGCTCATGGTCCTACATTGTGGAAACAT4020.11535845775498667No Hit
GCTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACT4000.11478453507958873No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA4000.11478453507958873No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC3950.11334972839109386No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA3920.11248884437799694No Hit
GCTTTACCCCATCTATTTCTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT3890.11162796036490004No Hit
TTCACCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCA3870.1110540376895021No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC3860.11076707635180312No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG3840.11019315367640518No Hit
GTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATTTGAGTCAGTC3840.11019315367640518No Hit
GAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTT3790.10875834698791031No Hit
GGTGTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGA3790.10875834698791031No Hit
GTGTTACCCAGGAGATTTCATCGATTATGAGGAGCTAAGAGAGCAATTGA3790.10875834698791031No Hit
TCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAGTTA3790.10875834698791031No Hit
TCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAATGGCCC3780.10847138565021136No Hit
AGTTCAGACAATGGAACGTGTTACCCAGGAGATTTCATCGATTATGAGGA3750.10761050163711444No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC3740.10732354029941546No Hit
TGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAA3720.10674961762401752No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT3710.10646265628631854No Hit
TGCACACACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCA3710.10646265628631854No Hit
ATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATCTACC3640.10445392692242574No Hit
TGCATATCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGT3630.10416696558472677No Hit
ATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACA3620.1038800042470278No Hit
CTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGATGGTGATAAC3570.10244519755853293No Hit
GTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAAT3540.10158431354543601No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG3530.10129735220773706No Hit
ATTGAGCTCAGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAA3520.10101039087003809No Hit
GTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGC3520.10101039087003809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)