FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Control08_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Control08_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGAGCTACGTTACCAGAACCTGAAATAACCACTTTCTTACCAGCAA12.5No Hit
ATTGTCAGGAATTAAAAAAACAAGTCTCAGGAAATCTGTCTCTTATACAC12.5No Hit
ATGCACACCTCACAAGGGTGTTTCTCAGAAAGCTTCTGTGTAGTTTTTAT12.5No Hit
GAGATTACAAAACAGCGCGAAGAATACTATCATCAGCTGTTTTATCTCGG12.5No Hit
TCCGAAAAGCGCGCGCGGCCGCGGAAGCTGTCTCTTATACACATCTCCGA12.5No Hit
AATTAGAAGAGATGCTCTTTTCCAAGCTATAATGGATGCTTTGTTTAATG12.5No Hit
GAGATATGTCTCAGTTGTCTTCACCCTTGGAACCTATGTTGACCTTATTT12.5No Hit
CCACAGCAACAAACTGAAGAATCCAATACACTAACCCCAAACACTAACCA12.5No Hit
GCTTCACAGACTTTGATTTTTAAGTCCAAGGTTACAGAGCTAGTAATTGA12.5No Hit
ATCATGGTGACGAAGGTGCGCACGATCGGTACGAAACGGGCCAGGATGAG12.5No Hit
ATGGAATGGAACGGAATGGAATGGAATGGAATGGAATGGAATAGAATGGT12.5No Hit
GTTCTGAAAGTTCTAATTGCGTATTCATTGCTTCTTCCAGCTGCTGTCTC12.5No Hit
GGTAAAGATAGAGTGTAACTTGATTTTTGCTTCTTCTATGTAGCTATTCA12.5No Hit
CTTCTGCTGATGGTCCAGTTTTCTCTGTCACCTAATTGGATCATCAGCTG12.5No Hit
CTGTTGTACCAGCCAACATGCAGACGATTTTGGATGAGAATGTAGCAGAG12.5No Hit
TTACAAAGCTTGCCTAAATTCACATGGATTAAAAGGTGGAGCTGGTATTC12.5No Hit
TTGTTGTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTC12.5No Hit
CAACAGCCACCTTTAAAATCAATTTCCAAGCATAAGGCATGACCTTGGCT12.5No Hit
TCAGAGAACCAAAGGGTTTCCATATCATTAGGTGAAGGATACTCCCCTGT12.5No Hit
GGTTAATTCCATTCCATTCCATTCCATTCCATTCCATTCCAATCGAGTTG12.5No Hit
GGATATAGGAATTTCCTGAAGACTTTCTGTGGTCTAATCTGCCTTATATT12.5No Hit
GTCTCTGACACTTCGGGCATCGGAACGAGCTCCGGTTCATGAAAGACTCG12.5No Hit
GGCTGATATCAGCCATGACGTCAGCTACGCTCTGCAGTCAGCTGTTTCTT12.5No Hit
GAAGATGCCTGTTTAGTAAAGGTCTCAGCTAGAATCAGTACACCTGTCTC12.5No Hit
GAAGGAATGCAGCCCTGCTGACACCTTGACTTTAGCCTTAATAGACCTAG12.5No Hit
TTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTT12.5No Hit
TTCATGACCTTCAACATTAACGAGATTCACGTTATTGTCATCCTGTCTCT12.5No Hit
GCTGCACTATTCACCATAGCAAAGACTTGGAAGCAACCTAAATGTCCAAC12.5No Hit
CATAAACTCTTTTCCATGTGAAATTGCTTCTTGTTTAGCCCTCCTCTGGA12.5No Hit
GAGCCATTGCGCTTCAGCGGCAGGTACGGTGTCGCCACCAGCCATGCGCC12.5No Hit
ATGGAATGGAATGGAATGGAATGGAATGGTATGGAATAGAATGGAATGGA12.5No Hit
ACTTCACACCCACTATCATGTGGGATTTGGGATTTGGGAACTTGATTATC12.5No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG12.5No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA12.5No Hit
CCCCAGGCTGATGTGGGTCAGCAAATGCTGGCGGATCACCACCGATGGCT12.5No Hit
AAGTGTTAAGTTCAACTGTTTTAGCTACTGCCATTTCTGTAACTACGATC12.5No Hit
AAGCATGACCTCACCAAATGAAGTTTATTTACTTCATTTGGTGAGGTCAT12.5No Hit
TAGTTCCCCCATGCAATTCACAGGTATTCTCTTACATAGAGATCACACTT12.5No Hit
CTGTTGCGCATTTGTCAGAGAGCATCACCCAGCTGAATCTCAGTGGCTGC12.5No Hit
CACTACTTGTCACATAACTACGGTCAATCCAAGTTTCCGTAGAGAGGTAG12.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)