FastQCFastQC Report
Thu 23 Dec 2021
000000000-K4LKV_l01_n01_SynCon_Control04_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4LKV_l01_n01_SynCon_Control04_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185355
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG10140.5470583474953468No Hit
CTATAGAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9440.5092929783388633No Hit
GTCCTACATTGTGGAAACATCTAGTTCAGACAATGGAACGTGTTACCCAG8330.4494078929621537No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC6690.3609290280812495No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC6130.33071673275606267No Hit
GTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCATTC5650.30482047962018827No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA5340.28809581613660273No Hit
CTATAGATGCTTCTGAAATCCTAATGTTAAATACATATTCTACACTGTAG4680.25248846807477543No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC4670.2519489628011114No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA4410.2379218256858461No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTC4350.23468479404386175No Hit
ATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG4280.23090825712821342No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC4150.22389468857058076No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4150.22389468857058076No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA4100.22119716220226054No Hit
TTCCAATTGTGATCGGATGTATATTCTGAAATGGGAGGCTGGTGTTTATA3980.21472309891829192No Hit
GTGATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA3660.19745893016104235No Hit
GCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGT3640.1963799196137142No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA3590.19368239324539396No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT3580.19314288797172993No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA3570.19260338269806587No Hit
GGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATG3520.18990585632974563No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG3500.18882684578241754No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC3460.18666882468776133No Hit
TACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATGTTT3430.18505030886676918No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA3410.18397129831944106No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA3400.18343179304577703No Hit
GTTAGTGGATGGGCTATATACAGTAAAGACAACAGTATAAGAATCGGTTC3370.18181327722478485No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA3350.1807342666774568No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3300.17803674030913652No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA3190.17210218229883198No Hit
GTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA3140.16940465593051174No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC3120.16832564538318362No Hit
GTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATTTGAGTCAGTC3060.16508861374119932No Hit
ATTAAAGACAGGAGCCCATATCGAACCCTAATGAGCTGTCCTATTGGTGA3030.16347009792020717No Hit
CAATATGGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAA2990.161312076825551No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT2880.15537751881524642No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG2860.1542985082679183No Hit
ATCTTGATGTCCCCACAAAAACATATGCATCTGCATTCTGATAGAGACTT2830.15267999244692618No Hit
ATATACAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGTGTT2820.15214048717326212No Hit
CCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGAC2800.15106147662593403No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT2760.14890345553127782No Hit
GTATGAGGCCTGTCCATCACTTGGTCCATCGGTCATTACAGTAAAGCAAG2750.14836395025761379No Hit
CTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGAGTCTGAATGT2740.1478244449839497No Hit
AATTATCACTATGAGGAATGCTCCTGTTATCCTGATTCTAGTGAAATCAC2740.1478244449839497No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT2740.1478244449839497No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA2730.14728493971028567No Hit
GTAATGACCGATGGACCAAGTGATGGACAGGCCTCATACAAGATCTTCAG2690.14512691861562946No Hit
CTATAGTTTTTTTGAACAAATTACTTGTCAATGGTAAATGGCAACTCAGC2650.14296889752097328No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA2640.14242939224730922No Hit
CTAATGAGCTGTCCTATTGGTGAAGTTCCCTCTCCATACAACTCAAGATT2600.14027137115265303No Hit
GCATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACG2570.13865285533166086No Hit
TTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGG2550.13757384478433277No Hit
GCACTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCC2540.1370343395106687No Hit
GTTTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTG2500.1348763184160125No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG2500.1348763184160125No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG2500.1348763184160125No Hit
ATCCAAACACCATTGCCGTATTTGAATGAAAATCCTTTTACTCCATTTGC2490.13433681314234847No Hit
GGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTTCCATTGCGAA2470.13325780259502037No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA2460.13271829732135632No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG2450.13217879204769226No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA2420.1305602762267001No Hit
TACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGAC2360.12732324458471583No Hit
TTATAGCACCCTTGGGTGTCTGACAAGTTGTATTGCAATCGTGGACTGGT2300.12408621294273152No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA2300.12408621294273152No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA2290.12354670766906747No Hit
GTGATAATTAGGGGCATTCATTTCGACTGATTTGACTATCTTTCCCTTTT2280.12300720239540341No Hit
CAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCATAAGGGAACCAT2270.12246769712173937No Hit
AGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTG2260.12192819184807531No Hit
GATAATACCAGATCCAGCATTTCTTTCCATTGCGAATGCATATCTCGGTA2260.12192819184807531No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA2240.12084918130074722No Hit
ATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCCAGCATTTCTTT2240.12084918130074722No Hit
GGATTAGCCACTCAATTCAACTTGGGAATCAAAATCAGATTGAAACATGC2190.11815165493242698No Hit
GTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACCAAGTGATGGA2190.11815165493242698No Hit
GTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATC2180.1176121496587629No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC2180.1176121496587629No Hit
CTAGTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTT2170.11707264438509887No Hit
CATCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAA2170.11707264438509887No Hit
AAATAGGCCTCTAGATTGAATAGACGGGACATTCCTCAATCCTGTGGCCA2170.11707264438509887No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT2170.11707264438509887No Hit
GTCTTATCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTGCATATGTA2160.11653313911143481No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA2130.11491462329044266No Hit
ATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAAGA2130.11491462329044266No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATGCCGTTGTAC2110.11383561274311456No Hit
GTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCT2110.11383561274311456No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAATACGGCAATG2080.11221709692212241No Hit
ATCCTACATTAATGATAAAGGGAAAGAAGTCCTCGTGCTATGGGGCATTC2080.11221709692212241No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA2070.11167759164845838No Hit
CTCTACTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTT2060.1111380863747943No Hit
CTGCATATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCA2050.11059858110113027No Hit
ATCCTGTGGCCAGTCTCAATTTTGTGCTTTTTACATATTTTGGACATTTT2040.11005907582746621No Hit
ACAATATATTGAGAACACAAGAGTCTGAATGTGCATGTGTAAATGGTTCT2040.11005907582746621No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATATCCTG2040.11005907582746621No Hit
ATTATGAGGAGCTAAGAGAGCAATTGAGCTCAGTGTCATCATTTGAAAGG2030.10951957055380217No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT2030.10951957055380217No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT2030.10951957055380217No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC2020.10898006528013812No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG2020.10898006528013812No Hit
TCATTAGGGCGTGGATTGTCTCCGAAAATCCCACTGCATATGTATCCTAT2020.10898006528013812No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT2010.10844056000647406No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG2010.10844056000647406No Hit
CAGTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTA2000.10790105473281002No Hit
TCAAGATACAGCAAGAAGTTCAAGCCGGAAATAGCAATAAGACCCAAAGT2000.10790105473281002No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC2000.10790105473281002No Hit
GCATATCCTGACCCCTGCTCATTTTGATGGTGATAACCGTACCATCCATC1980.10682204418548191No Hit
TCTCCATACAACTCAAGATTTGAGTCAGTCGCTTGGTCAGCAAGTGCTTG1980.10682204418548191No Hit
GTAACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATG1970.10628253891181785No Hit
CTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATTCCAATTGTTA1970.10628253891181785No Hit
TAATAACAGACACTATCAAGAGTTGGAGAAACAATATATTGAGAACACAA1960.10574303363815381No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC1940.1046640230908257No Hit
CAGTAAAGACAACAGTATAAGAATCGGTTCCAAGGGGGATGTGTTTGTCA1940.1046640230908257No Hit
ATGTAGGATTTGCTGAGCTTTGGGTATGAATTTCCTTTTTTAACTAGCCA1940.1046640230908257No Hit
GTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAACA1930.10412451781716167No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC1910.10304550726983357No Hit
GAATAGACGGGACATTCCTCAATCCTGTGGCCAGTCTCAATTTTGTGCTT1910.10304550726983357No Hit
CACATGTGATTTCACTAGAATCAGGATAACAGGAGCATTCCTCATAGTGA1910.10304550726983357No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAT1910.10304550726983357No Hit
AACTAAGAGGGGTAGCCCCATTGCATTTGGGTAAATGTAACATTGCTGGC1900.10250600199616952No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC1890.10196649672250546No Hit
TTCCTGGACATTTGGACTTACAATGCCGAACTGTTGGTTCTATTGGAAAA1860.10034798090151331No Hit
GTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTT1860.10034798090151331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)