FastQCFastQC Report
Mon 15 Nov 2021
000000000-K4JTH_l01_n01_section7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-K4JTH_l01_n01_section7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373555
Sequences flagged as poor quality0
Sequence length251
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGT22880.6124934748564469TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC16020.4288525116783339TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)