FastQCFastQC Report
Tue 21 Dec 2021
000000000-JVJ4T_l01_n02_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JVJ4T_l01_n02_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2698524
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1035433.8370234987719214Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC69950.25921577869976326Illumina Single End PCR Primer 1 (100% over 38bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG68200.2527307520703911Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC36320.134592095530742Illumina Single End PCR Primer 1 (100% over 40bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG26990.1000176392724319Illumina Single End PCR Primer 1 (97% over 48bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)