FastQCFastQC Report
Tue 21 Dec 2021
000000000-JVJ4T_l01_n02_28B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JVJ4T_l01_n02_28B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4341930
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1778314.095667134200689Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC141050.324855536593174Illumina Single End PCR Primer 1 (100% over 38bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG107630.2478851570614911Illumina Single End PCR Primer 1 (100% over 50bp)
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT80510.185424454102208No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC62950.1449816095607253Illumina Single End PCR Primer 1 (100% over 40bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG43960.1012452987496344Illumina Single End PCR Primer 1 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)