FastQCFastQC Report
Tue 21 Dec 2021
000000000-JVJ4T_l01_n01_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-JVJ4T_l01_n01_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2698524
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG1029453.8148632363469805TruSeq Adapter, Index 22 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA102490.3798002167110613TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA72530.26877656081620915TruSeq Adapter, Index 22 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC63960.2370184589797978TruSeq Adapter, Index 22 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGACCTCGTATG44190.16375618671540443TruSeq Adapter, Index 22 (97% over 39bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACG37130.13759373642776568TruSeq Adapter, Index 22 (100% over 39bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC33820.125327771774496TruSeq Adapter, Index 22 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGAACTCGTATG28020.10383454066000525TruSeq Adapter, Index 22 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)